1. A modification of Steedman's ester wax embedding method is described. This involved purification of two of the components--diethylene glycol monostearate and distearate. The puckering and floating off of the sections is thereby minimized.
2. The method is particularly applicable to tissues containing much collagen and to tissues that have been badly fixed or which have undergone post-mortem degeneration.
3. Cytological preservation is improved, especially that of such substances as glycoproteins and mucoproteins.