A method for making squash preparations of plant root tip cells is described. This entails the use of an inert preserving substance, polyvinyl alcohol. The advantages of the method are the preservation of the cytoplasmic bodies, including the mitochondria ; the retention of fat droplets inside the cells; the fact that the nucleus remains optically homogeneous; and the fixing of free aldehyde groups and of diffusible substances in the cytoplasm. It is a relatively rapid and simple technique, and the cells produced by it resemble closely those produced by the method for making living squash preparations of such cells.

This content is only available via PDF.