The phosphatases in the cytoplasm and nuclei of Drosophila salivary glands are better preserved by fixation in absolute acetone than in 85 per cent, alcohol. In whole glands there is relatively little extraction of the enzyme during assay. Phosphatase activity is more resistant to incubation at neutrality than at pH 8.6, but in this material there is sufficient residual enzymatic activity to permit redetermination of alkaline, neutral, or acid phosphatase activity by staining methods after an initial quantitative determination. The state of the membranes of the gland affects the penetration of the substrate sufficiently to limit the activities obtained.
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JOURNAL ARTICLE| 01 December 1948
Phosphatase Activity of Drosophila Salivary Glands
W. L. DOYLE
Department of Anatomy, University of Chicago
Online Issn: 1477-9137
Print Issn: 0021-9533
Copyright © 1948 by the Company of Biologists Ltd.
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W. L. DOYLE; Phosphatase Activity of Drosophila Salivary Glands. J Cell Sci 1 December 1948; s3-89 (8): 415–419. doi: https://doi.org/10.1242/jcs.s3-89.8.415
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