The effect of fixation with acidified solutions of osmium tetroxide (pH 1.5 to 3.5 has been studied on the first (proximal) convoluted tubule cells of the kidney and the pancreatic exocrine cells of the mouse, by electron microscopy. Partially prepolymerized methacrylate was used for embedding. The various membranous structures and the ribosomes retain their individuality even after prolonged fixation in solutions containing 5% acetic acid (pH 1.5). However, the mitochondrial matrix and the ground cytoplasm are not preserved; the zymogen granules are also partially washed out.
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JOURNAL ARTICLE| 01 March 1963
Experiments on Fixation for Electron Microscopy : 3. A Note on Fixation in Acidified Osmium Tetroxide
S. K. MALHOTRA
Cytological Laboratory, Department of Zoology, University Museum, Oxford
Online Issn: 1477-9137
Print Issn: 0021-9533
Copyright © 1963 by the Company of Biologists Ltd.
J Cell Sci (1963) s3-104 (65): 123–127.
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S. K. MALHOTRA; Experiments on Fixation for Electron Microscopy : 3. A Note on Fixation in Acidified Osmium Tetroxide. J Cell Sci 1 March 1963; s3-104 (65): 123–127. doi: https://doi.org/10.1242/jcs.s3-104.65.123
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