The effect of fixation with osmium tetroxide solution, made alkaline by the addition of potassium acetate, was studied by electron microscopy. Exocrine cells of the pancreas and the cells of the first (‘proximal’) convoluted tubules of the kidney of the mouse were used as test-objects. Partially prepolymerized methacrylate was used for embedding. The preservation of the various cell inclusions was similar to what is generally produced after fixation with Palade's buffered osmium tetroxide.
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JOURNAL ARTICLE| 01 September 1962
Experiments on Fixation for Electron Microscopy 2. Alkalized Osmium Tetroxide
S. K. MALHOTRA
Cytological Laboratory, Department of Zoology, University Museum, Oxford
Online Issn: 1477-9137
Print Issn: 0021-9533
Copyright © 1962 by the Company of Biologists Ltd.
J Cell Sci (1962) s3-103 (63): 287–296.
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S. K. MALHOTRA; Experiments on Fixation for Electron Microscopy 2. Alkalized Osmium Tetroxide. J Cell Sci 1 September 1962; s3-103 (63): 287–296. doi: https://doi.org/10.1242/jcs.s3-103.63.287
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