The effect of fixation with a simple solution of osmium tetroxide in distilled water was studied by electron microscopy. Exocrine cells of the pancreas and cells of the proximal tubule of the kidney of the mouse were used as test-objects. Partially prepolymerized methacrylate was used for embedding. There did not appear to be any marked disorganization of the cell inclusions. The appearance of the inclusions in the electron micrographs was similar to what is generally seen after fixation with the buffered osmium tetroxide solution of Palade.
Skip Nav Destination
JOURNAL ARTICLE| 01 March 1962
Experiments on Fixation for Electron Microscopy : 1. Unbuffered Osmium Tetroxide
S. K. MALHOTRA
The Cytological Laboratory, Department of Zoology, University Museum, Oxford
Online Issn: 1477-9137
Print Issn: 0021-9533
Copyright © 1962 by the Company of Biologists Ltd.
J Cell Sci (1962) s3-103 (61): 5–15.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Search Site
S. K. MALHOTRA; Experiments on Fixation for Electron Microscopy : 1. Unbuffered Osmium Tetroxide. J Cell Sci 1 March 1962; s3-103 (61): 5–15. doi: https://doi.org/10.1242/jcs.s3-103.61.5
Download citation file: