The chorio-allantoic membranes of White Leghorn chick embryos at 10 to 12 days after laying were fixed in Palade's buffered osmium tetroxide or in Luft's potassium permanganate.

After fixation in these two ways the general appearance is similar, but there are differences in certain tissue elements. Cell membranes are well preserved after fixation in KMnCv Certain lipid inclusions in the cytoplasm of the cells of the allantoic layer are well seen after fixation by OsO4, but not after KMnO4; in their places empty vacuoles are seen.

Details of the structure of the red blood-corpuscles are more clearly seen after fixation in KMnO4.

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