Two main modifications of Holmes's method are described. In both the amount of silver nitrate in the impregnating bath is increased, and larger quantities of higher pyridine derivatives are substituted for pyridine. The preferred method for general purposes employs 2.4% 2:6 lutidine at pH 8.4, and gives a bluish picture with improved selectivity of fibre staining. The use of 1.6% 2:4:6 collidine gives a reddish picture, with highly selective fibre staining, and sharp resolution of neurosecretory cells. Minor modifications of pre-treatment and reduction procedures are also described.
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JOURNAL ARTICLE| 01 September 1961
Some Modifications of Holmes's Silver Method for Insect Central Nervous Systems
A. D. BLEST
Department of Zoology and Comparative Anatomy, University College, London, W.C. 1
Online Issn: 1477-9137
Print Issn: 0021-9533
Copyright © 1961 by the Company of Biologists Ltd.
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A. D. BLEST; Some Modifications of Holmes's Silver Method for Insect Central Nervous Systems. J Cell Sci 1 September 1961; s3-102 (59): 413–417. doi: https://doi.org/10.1242/jcs.s3-102.59.413
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