1. Peranema trichophorum is holozoic in nature and selective in its food, but not predaceous. It feeds usually on dead and encysted Euglena proxima, Euglena gracilis, and rarely upon Chilomonas and Entosiphon.
2. The ‘Staborgan’ or rod-organ is not connected with the reservoir, but it opens into the cytostome which lies ventrally to this vesicle. Therefore the term gullet should not be applied to the neck of the reservoir.
3. The chromosome count of Peranema trichophorum is estimated to be thirty-two in number.
4. The ‘Staborgan’ is thrown out of its position during mitosis and it disintegrates in the cytoplasm. New rod-organs grow out from granules which form at the base of the new daughter cytostomes. These granules may be of mitochondrial origin.
5. A centroblepharoplast is described. No paradesmose is present.
6. A theory is suggested which supposes that an interaction between the centroblepharoplast and the endosome occurs. The centroblepharoplast acts as a kinetic attraction sphere which carries the nucleus anteriorly in order that the blepharoplasts can function as extra-nuclear division centres; thereby a co-ordinated interaction is brought about between both intranuclear kinetic elements and all of its cellular components. Such a reaction or interrelation of parts is necessary to initiate cellular division.
7. The mitochondria of Peranema were found to be spherical; these may grow into large disc-shaped types with clear centres. The latter have a tendency to group themselves round the nucleus and the reservoir.
8. The Golgi apparatus was found to be a network of long fibres. These Golgi bodies seem to be concentrated in the posterior end and round the reservoir. Neither the contractile vacuole nor the reservoir was impregnated by osmic acid methods.