The enteric nervous system (ENS) consists of an extensive network of neurons and glial cells embedded within the wall of the gastrointestinal (GI) tract. Alterations in neuronal distribution and function are strongly associated with GI dysfunction. Current methods for assessing neuronal distribution suffer from undersampling, partly due to challenges associated with imaging and analyzing large tissue areas, and operator bias due to manual analysis. We present the Gut Analysis Toolbox (GAT), an image analysis tool designed for characterization of enteric neurons and their neurochemical coding using 2D images of GI wholemount preparations. It is developed in Fiji, has a user-friendly interface and offers rapid and accurate segmentation via custom deep learning (DL) based cell segmentation models developed using StarDist, and a ganglion segmentation model in deepImageJ. We use proximal neighbor-based spatial analysis to reveal differences in cellular distribution across gut regions using a public dataset. In summary, GAT provides an easy-to-use toolbox to streamline routine image analysis tasks in ENS research. GAT enhances throughput allowing unbiased analysis of larger tissue areas, multiple neuronal markers and numerous samples rapidly.
Gut Analysis Toolbox: Automating quantitative analysis of enteric neurons
Call for papers: Cell Biology of Mitochondria
We are welcoming submissions for our upcoming special issue: Cell Biology of Mitochondria. This issue will be coordinated by two Guest Editors: Ana J. Garcia-Saez (University of Cologne, Germany) and Heidi McBride (McGill University, Canada). Submission deadline: 1 October 2024.
Focal adhesion kinase signalling – tumour vulnerabilities and clinical opportunities
In this Review, David Schlaepfer and colleagues summarise 30 years of focal adhesion kinase (FAK) research with a view of the ongoing clinical testing of small-molecule FAK inhibitors. The authors touch on how FAK plays an important signal integration role and ultimately functions to guide cellular behaviour. Additionally, the authors discuss how FAK inhibition might present a powerful tool to influence the physiological response to other therapeutic approaches.
JCS-FocalPlane Training Grants
Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, please apply. Deadline dates for 2024 applications: 7 September (decision by week commencing 8 October 2024); 22 November (decision by week commencing 16 December).
Biologists @ 100 - join us in Liverpool in March 2025
We are excited to invite you to a unique scientific conference, celebrating the 100-year anniversary of The Company of Biologists, and bringing together our different communities. The conference will incorporate the Spring Meetings of the BSCB and the BSDB, the JEB Symposium Sensory Perception in a Changing World and a DMM programme on antimicrobial resistance. Find out more and register your interest to join us in March 2025 in Liverpool, UK.
Interview with Journal of Cell Science Editor Rob Parton
Read our interview with Rob Parton, a Cell Scientist to Watch, about his career journey leading him from the UK to the University of Queensland in Brisbane, Australia, the evolution of the membrane trafficking field and his advice for running a highly collaborative lab. As a Journal of Cell Science Editor, Rob brings to the journal his expertise in multiscale analysis of membrane function, membrane microdomains, lipid droplets and advanced microscopy techniques in cell biology.
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