gamma-Tubulin exists in fission yeast as the product of an essential gene, encoding a 446 amino acid protein that is 77.3% identical to Aspergillus nidulans gamma-tubulin. The gene disruption caused cell lethality, displaying condensed, undivided chromosomes with aberrant spindle structures. Anti-gamma-tubulin staining showed that gamma-tubulin is located, throughout the wild-type cell cycle, at the spindle pole bodies (SPBs), indicating that gamma-tubulin associates with interphase SPB in the absence of microtubules. In addition, anti-gamma-tubulin immunofluorescence staining revealed cytoplasmic, cell-equatorial putative MTOCs (microtubule organizing centers), which appear only during mitotic telophase and cytokinesis, and are located at the centers for the new cytoplasmic microtubule arrays of the two daughter cells. In the multiple-SPB mutant cut1-cdc11, anti-gamma-tubulin antibodies revealed many dots on the periphery of the nucleus. These results confirm that gamma-tubulin is an important member of the tubulin superfamily, suggest that it may be a universal component of MTOCs, and are consistent with a role for gamma-tubulin in controlling microtubule formation in vivo.
2021 JCS Prize winner announced
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We are pleased to announce that the winner of the 2021 JCS Prize is Lee Dolat for his paper entitled ‘An endometrial organoid model of interactions between Chlamydia and epithelial and immune cells’.
Find out more about our preprint policies
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Last year, 47% of the manuscripts published in Journal of Cell Science were posted as preprints on bioRxiv. You can find our preprint policies here.
Cell scientist to watch: Christian Münch
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Journal of Cell Science interviewed Christian Münch, who established his independent research group in 2016 at Goethe University Frankfurt. His lab studies cellular stress responses to mitochondrial protein misfolding, infection and disease, as well as developing proteomics methods.
A unifying model for the role of the ATG8 system in autophagy
In this Hypothesis article, Thanh Ngoc Nguyen and Michael Lazarou reconcile prior observations of the core ATG8 system into a unifying model of their function that aims to consider apparently conflicting discoveries.
FocalPlane Network launched
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We are excited to announce the launch of the FocalPlane Network, an international directory of microscopists. The idea behind the FocalPlane Network is to facilitate promotion and networking as well as assist those seeking conference speakers, committee members, reviewers or collaborators. We hope that it will help promote diversity in the community. Find out more and join the Network here.
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