The gastrulating chick blastoderm contains lectin activity specific for beta-D-galactoside groups. The galactose-binding lectin isolated by affinity chromatography on rho-aminophenyl-beta-D-lactoside separates into two bands when studied by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. One of these LII has a relative molecular mass of 70 (+/− 2) X 10(3) while the other LI is a polypeptide that migrates with the dye front in 10% gels. We have prepared an antiserum against this lectin preparation and have affinity-purified antibodies against LI. When embryos at stages 3–7 were examined by immunofluorescence using the affinity-purified antibodies, lectin was expressed in cells at the lowest portions of the primitive streak as well as in cells migrating laterally from this region to form the endoderm. Lectin was also expressed by the cells of the extra-embryonic endoderm and the primordial germ cells of the proximal area opaca. In transfers of gradient gels stained with affinity-purified antibodies against LI, this lectin had an approximate molecular weight of 6.5 X 10(3). Our results indicate that this lectin is expressed in areas that are undergoing cell spreading.
Cell Scientists to Watch
Talented researchers who have recently set up their own labs talk about their lives in and out of the lab, the journeys that led them to where they are now, the current state of science funding, and give advice on how to navigate the transition to independence.
'FocalPlane features...' webinar series
We’re excited to announce the new 'FocalPlane features…' webinar series. Covering all aspects of microscopy, join us on the first Tuesday of every month to hear about the latest research and take part in a virtual networking session with the speaker afterwards.
The Corona Files
“In the past year we have called out inequality and injustice, we have forged a public awareness of the value of science, and we have found new ways to interact around the world."
Our resident insectivore, Mole, continues his latest series – The Corona Files. Catch up on Mole’s weekly musings on how COVID-19 is changing the landscape for researchers.
Read & Publish voices: Chris MacDonald
JCS joins the Preprint Reviewer Recruitment Network
Journal of Cell Science, along with its sister journals Development and Biology Open, joins ASAPbio's Preprint Reviewer Recruitment Network, a new initiative aiming to help researchers, journals and publishers, by enabling willing researchers to share preprint feedback as work samples for review by participating journals.
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