Mitochondrial nucleoids (mt-nucleoids) of the yeast, Saccharomyces cerevisiae, were isolated from spheroplasts of stationary phase cells and their structure and organization were investigated by fluorescence microscopy, electron microscopy, and biochemical techniques. Isolated mt-nucleoids were spherical or ovoid and 0.3-0.6 micron in diameter, and were about the same size and shape as those observed in the cell by the DAPI staining technique. Measurement of DNA content of mt-nucleoids, using a video-intensified microscope system, after DAPI staining revealed that a mt-nucleoid in spheroplasts from stationary phase cells contains, on average, 3.9 mtDNA molecules and an isolated mt-nucleoid contains, on average, 3.1. Negatively stained electron micrographs showed that mt-nucleoids consist of particles 20–50 nm in diameter. SDS-polyacrylamide gel electrophoresis of mt-nucleoids detected 20 species of polypeptides in the molecular weight range from 10 X 10(3) to 70 X 10(3). Acid-urea/SDS two-dimensional electrophoresis of acid extract from mt-nucleoids indicated that a polypeptide of 20 X 10(3) is the only major polypeptide with basic property like histones.
Cell Scientists to Watch
Talented researchers who have recently set up their own labs talk about their lives in and out of the lab, the journeys that led them to where they are now, the current state of science funding, and give advice on how to navigate the transition to independence.
'FocalPlane features...' webinar series
We’re excited to announce the new 'FocalPlane features…' webinar series. Covering all aspects of microscopy, join us on the first Tuesday of every month to hear about the latest research and take part in a virtual networking session with the speaker afterwards.
The Corona Files
“In the past year we have called out inequality and injustice, we have forged a public awareness of the value of science, and we have found new ways to interact around the world."
Our resident insectivore, Mole, continues his latest series – The Corona Files. Catch up on Mole’s weekly musings on how COVID-19 is changing the landscape for researchers.
Read & Publish voices: Chris MacDonald
JCS joins the Preprint Reviewer Recruitment Network
Journal of Cell Science, along with its sister journals Development and Biology Open, joins ASAPbio's Preprint Reviewer Recruitment Network, a new initiative aiming to help researchers, journals and publishers, by enabling willing researchers to share preprint feedback as work samples for review by participating journals.
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