Trypsin digestion of demembranated fowl spermatozoa caused a longitudinal splitting of the distal part of the axoneme. The resulting strands, consisting of groups of doublet microtubules, formed left-handed helices. On the evidence of electron micrographs, the digestion had caused the loss of dynein arms from the outer row; it is assumed that the doublets remained linked together by dynein arms of the inner row. When such helices were mechanically detached from the proximal flagellum and reactivated with adenosine triphosphate, they lengthened in an orderly way by inter-doublet sliding. All the doublets of the axoneme could be reactivated and in all instances the direction of sliding was the same as that reported for the cilia of Tetrahymena. Within the groups of doublets the measured inter-doublet displacements were generally similar, suggesting that the rates of sliding had been equivalent. These findings are contrasted with the differential pattern of activation that is assumed to occur in vivo.
Direction of sliding and relative sliding velocities within trypsinized sperm axonemes of Gallus domesticus
D.M. Woolley, A. Brammall; Direction of sliding and relative sliding velocities within trypsinized sperm axonemes of Gallus domesticus. J Cell Sci 1 October 1987; 88 (3): 361–371. doi: https://doi.org/10.1242/jcs.88.3.361
Download citation file:
Reasons to submit to Journal of Cell Science
There are many benefits to publishing through Journal of Cell Science, follow this link to read more about our reasons to submit or visit our submission page now.
Call for papers: Cell and Tissue Polarity
We are welcoming submissions for our next special issue, which will focus on ‘Cell and tissue polarity’ and will be guest edited by David Bryant. Submission deadline: 15 July.
The Forest of Biologists
The Forest of Biologists is a biodiversity initiative created by The Company of Biologists, with support from the Woodland Trust. For every Research and Review article published in Journal of Cell Science a native tree is planted in a UK forest. In addition to this we are protecting and restoring ancient woodland and are dedicating these trees to our peer reviewers. Visit our virtual forest to learn more.
Propose a new Workshop for 2025
Do you have an idea for a Workshop? We are now accepting proposals for our 2025 Biologists Workshops programme. As the scientific organiser, your involvement will be focused on the science. We'll take care of all the logistics. In 2025 we'll continue our efforts to diversify our Workshop programme and will be reserving one of our Workshops for an application from a Global South (GS) country to host an event overseas.
FocalPlane Image Competition 2023
FocalPlane’s 2023 image competition is underway, in celebration of the community site’s third birthday. Click here for submission details and information on how to showcase your favourite image.