The quantity of c-myc mRNA was measured during the retinoic-acid-induced differentiation of the pluripotent human teratoma cell line, Tera-2 cl. 13. As the cells were exposed to retinoic acid for longer periods of time the duration of the cell cycle progressively increased (measured by the rate of S phase entry) until the cells were effectively quiescent and expressed characteristic differentiation markers. Under these circumstances steady-state levels of c-myc expression increased by up to 1.6-fold with respect to rapidly growing undifferentiated cells. Southern blot analysis of the c-myc genes in Tera-2 indicated no major rearrangement or amplification in the cell line.
Expression of c-myc during differentiation of the human teratocarcinoma cell line Tera-2
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P.N. Schofield, W. Engstrom, A.J. Lee, C. Biddle, C.F. Graham; Expression of c-myc during differentiation of the human teratocarcinoma cell line Tera-2. J Cell Sci 1 August 1987; 88 (1): 57–64. doi: https://doi.org/10.1242/jcs.88.1.57
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