A spontaneously transformed pulmonary embryonic rat epithelial cell line (PER) is described in terms of growth, tumorigenicity, growth factor responsiveness and biosynthetic capacity. At low-passage subcultures, PER cells grew as a monolayer and did not form colonies in soft agar. After long-term subcultivation, they lost contact inhibition, became anchorage-independent and formed tumours in nude mice. Low concentrations of foetal calf serum permit the maximum growth rate. The multiplication and metabolic activity, assessed by 2-deoxy-D-glucose uptake, was significantly stimulated by growth factors. PER cells synthesized collagen types I, III, IV and V, laminin and fibronectin, and organized a pericellular matrix made up of only basement membrane components (type IV collagen and laminin) and fibronectin. These data enabled us to define PER cells as a transformed epithelial cell line evolving towards malignancy with long-term subcultivation. These cells appeared to be a valuable tool in studies of tumour cell-matrix interactions and regulation of growth factor receptors in tumorigenesis.
Characterization of a spontaneously transformed pulmonary embryonic rat (PER) epithelial cell line
M. Paye, C. Etievant, F. Michiels, D. Pierard, B. Nusgens, C.M. Lapiere; Characterization of a spontaneously transformed pulmonary embryonic rat (PER) epithelial cell line. J Cell Sci 1 December 1986; 86 (1): 83–93. doi: https://doi.org/10.1242/jcs.86.1.83
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