Fifteen amicronucleate cell lines and 22 cell lines with defective micronuclei were obtained following selective laser microbeam irradiation of the micronucleus. The amicronucleate cell lines showed reduced growth rate and formed abnormal oral apparatuses in asexual reproduction, and failed to produce any oral apparatus in autogamy, in agreement with previous observations on amicronucleate cells obtained by micropipetting. The 22 cell lines with defective micronucleus exhibited various abnormalities of the oral apparatus newly formed during autogamy. These abnormalities included the arrest of membranelle assembly, reduction in the length of the buccal cavity and oral membranelles, disruption of the organization of the membranelles, quadrulation of the dorsal peniculus, and failure of addition of membranellar basal body rows. Hence the micronucleus plays multiple roles in sexual stomatogenesis, deciding early steps of oral membranelle assembly and affecting their subsequent patterning. Our results agree with the notion that the micronucleus acts during a critical period between the second meiotic division and up to the formation of the zygotic nucleus to control the early stage of oral membranelle assembly. Laser microbeam irradiation might have created recessive mutations and/or chromosomal aberrations, which were expressed during this critical period with the formation of abnormal postmeiotic nuclei.
Call for papers: Cell and Tissue Polarity
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We are welcoming submissions for our next special issue, which will focus on ‘Cell and tissue polarity’ and will be guest edited by David Bryant. Submission deadline: 15 July.
The Forest of Biologists
We are excited to announce the launch of The Forest of Biologists, a new biodiversity initiative created with support from the Woodland Trust, aiming to counteract nature loss and safeguard some of the most critically endangered ecosystems for future generations. For every Research Article and Review/Commentary article that is published in Journal of Cell Science, a native tree is planted in a forest in the UK.
Propose a new Workshop for 2025
Do you have an idea for a Workshop? We are now accepting proposals for our 2025 Biologists Workshops programme. As the scientific organiser, your involvement will be focused on the science. We'll take care of all the logistics. In 2025 we'll continue our efforts to diversify our Workshop programme and will be reserving one of our Workshops for an application from a Global South (GS) country to host an event overseas.
Editorial: Publishing where it matters
Cell Scientists to Watch
As a community-focused journal, Journal of Cell Science is keen to support the next generation of cell biologists. Check out Cell Scientists to Watch, our interview series featuring talented researchers who have recently set up their own labs.
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