A culture perfusion system is described for the growth of anchorage-dependent cells on microcarriers. The critical component of this system is a column separator, which removes medium while allowing the microcarriers to remain in the culture. Amino acids and ammonia were analysed during cell growth of the perfusion culture. None of the amino acids was completely utilized. The accumulation of ammonia to 2.3 mM was observed and may be responsible for, or coincident with, events limiting further cell growth. It is suggested that oxygen deprivation and growth inhibitor accumulation, rather than nutrient depletion, are the major factors in limiting even higher cell yields.
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JOURNAL ARTICLE| 01 May 1983
High yields from microcarrier cultures by medium perfusion
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1983 by Company of Biologists
J Cell Sci (1983) 61 (1): 351–363.
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M. Butler, T. Imamura, J. Thomas, W.G. Thilly; High yields from microcarrier cultures by medium perfusion. J Cell Sci 1 May 1983; 61 (1): 351–363. doi: https://doi.org/10.1242/jcs.61.1.351
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