Synchronous cultures of Physarum polycephalum were pulsed with [3H]lysine hydrochloride in S and G2 phases of the cell cycle. Plasmodial extracts were separated into nuclear, ribosomal and acid-soluble post-ribosomal cytoplasmic fractions. Core histones could be detected by staining in the nuclear fractions of both S and G2 phases, but were not detected by staining in the cytoplasmic fractions. Newly synthesized histone was present in S-phase nuclei but not in S-phase cytoplasm. The specific activity of newly synthesized histone in G2-phase nuclei decreased by at least 95% compared to S phase and no newly synthesized histone was observed in G2-phase cytoplasmic fractions. Thus histone synthesis is restricted to S phase. There are no free pools of histone in the cytoplasm of Physarum in either S or G2 phases of the cell cycle.
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JOURNAL ARTICLE| 01 October 1982
Control of histone gene expression in Physarum polycephalum. I. Protein synthesis during the cell cycle
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1982 by Company of Biologists
J Cell Sci (1982) 57 (1): 139–150.
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P.N. Schofield, I.O. Walker; Control of histone gene expression in Physarum polycephalum. I. Protein synthesis during the cell cycle. J Cell Sci 1 October 1982; 57 (1): 139–150. doi: https://doi.org/10.1242/jcs.57.1.139
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