A hierarchy of relative cohesiveness in monolayer of four different embryonic chick tissues was determined in a previous study. The hierarchy is: corneal epithelium congruent to liver parenchyma greater than pigmented epithelium greater than limb bud mesenchyme. The purpose of this paper is to describe the correlation between these adhesive relationships and, firstly, the amount of the adhesive glycoprotein, fibronectin, associated with the cells and, secondly, the morphology of their intercellular contacts. Fluorescent antibody staining of the cells with anti-fibronectin antibody showed that limb bud mesenchyme cells, the most weakly cohesive, had much more fibronectin than the other cell types. Thus there was a negative correlation between the amount of fibronectin and cellular cohesiveness. Analysis of intercellular contacts by electron microscopy showed that the most strongly cohesive cell types, corneal epithelium and liver parenchyma, were also those that possessed desmosomes.
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JOURNAL ARTICLE| 01 April 1982
Fibronectin, intercellular junctions and the sorting-out of chick embryonic tissue cells in monolayer
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1982 by Company of Biologists
J Cell Sci (1982) 54 (1): 357–372.
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A. Nicol, D.R. Garrod; Fibronectin, intercellular junctions and the sorting-out of chick embryonic tissue cells in monolayer. J Cell Sci 1 April 1982; 54 (1): 357–372. doi: https://doi.org/10.1242/jcs.54.1.357
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