The effects of hyaluronate on rabbit neutrophil adhesion were studied using a variety of techniques. Exogenous hyaluronate inhibited neutrophil aggregation under conditions of both turbulent flow and constant shear rate. Hyaluronate also inhibited neutrophil adhesion to glass. Inhibition was dose-dependent above 100 micrograms ml-1 and a minimum molecular weight for hyaluronate of 1 × 10(4) was required. These effects were not simply the result of increased bulk viscosity of the hyaluronate-containing medium, nor did they appear to be mediated by putative cell-surface receptor mechanisms. Instead, physical factors such as hindrance and/or changes in the interfacial free-energy exchange at the cell surface due to the unusual hydrodynamic properties of the hyaluronate molecule were considered to be more important. Since neutrophil migration in vivo occurs through hyaluronate-rich connective tissue matrices, the relevance of these findings for processes such as inflammation and wound healing is clear.
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JOURNAL ARTICLE| 01 August 1981
Effect of hyaluronic acid on neutrophil adhesion
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1981 by Company of Biologists
J Cell Sci (1981) 50 (1): 329–344.
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J.V. Forrester, J.M. Lackie; Effect of hyaluronic acid on neutrophil adhesion. J Cell Sci 1 August 1981; 50 (1): 329–344. doi: https://doi.org/10.1242/jcs.50.1.329
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