A discontinuous sucrose density gradient was used to separate membrane fractions from a homogenate of maize root tips. Endoplasmic reticulum-, Golgi apparatus-, plasma membrane- and mitochondria-rich fractions were identified by their enzymic characteristics and by their appearance under the electron microscope. Maize roots were incubated in vivo with D-[U-14C]glucose, [Me-14C]choline chloride and diazotized [U-3H]sulphanilic acid. The pattern of incorporation of radioactivity into the various membrane fractions was investigated. Analyses of the polypeptide chains of the membrane fractions by SDS-polyacrylamide gel electrophoresis showed that the mitochondria-rich fraction had a different pattern of polypeptides from that of the other membrane fractions. The results are discussed in relation to the hypothesis of endomembrane flow and differentiation.
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JOURNAL ARTICLE| 01 October 1980
Isolation and characterization of membranes from the cells of maize root tips
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1980 by Company of Biologists
J Cell Sci (1980) 45 (1): 147–167.
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E.A. Baydoun, D.H. Northcote; Isolation and characterization of membranes from the cells of maize root tips. J Cell Sci 1 October 1980; 45 (1): 147–167. doi: https://doi.org/10.1242/jcs.45.1.147
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