We have developed a quantitative assay for tissue-specific adhesive components which is based on the agglutination of glutaraldehyde-fixed cells. At least 2 components are required for fixed-cell agglutination: a cell-surface ligand which is obtained from tissue culture-conditioned medium, and a soluble ‘agglutinin’ which accumulates in conditioned medium from monolayer cultures. Our results suggest that the surface-binding ligand and the agglutinin interact directly, resulting in tissue-specific agglutination of cells. The agglutination reaction exhibits divalent cation, temperature, and pH dependence. Several models of cell adhesion are described; the simplest of these which can account for the data is a multicomponent model in which the 2 adhesive components have structural roles.
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JOURNAL ARTICLE| 01 April 1979
Functional characterization of an adhesive component from the embryonic chick neural retina
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1979 by Company of Biologists
J Cell Sci (1979) 36 (1): 323–342.
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R. Rutz, J. Lilien; Functional characterization of an adhesive component from the embryonic chick neural retina. J Cell Sci 1 April 1979; 36 (1): 323–342. doi: https://doi.org/10.1242/jcs.36.1.323
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