The surface of a separate Dictyostelium discoideum cell exposed to fluorescent antiserum is initially uniformly stained. When the cell moves, labelled material is left on the glass over the area occupied by the cell while in the antiserum. The surface of new pseudopods is fluorescent. The bulk of the label soon accumulates on the rear of the cell and is towed along behind it. The rest of the surface may remain faintly fluorescent for long periods, but this residual label is immediately left behind if the cell advances while strongly compressed between agar and glass. This evidence does not clearly decide between the hypotheses of a permanent, advancing, lateral cell surface and a temporary, stationary one. The fact that the lateral surface of an advancing cell does not drag forward viscous medium in contact with it does not favour the former hypothesis.
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JOURNAL ARTICLE| 01 June 1968
Labelling of the Cell Surface of the Collective Amoeba Dictyostelium Discoideum with Fluorescent Antiserum
B. M. SHAFFER
Department of Zoology, University of Cambridge
Received: 31 Aug 1967
Online Issn: 1477-9137
Print Issn: 0021-9533
Copyright © 1968 The Company of Biologists Ltd.
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B. M. SHAFFER; Labelling of the Cell Surface of the Collective Amoeba Dictyostelium Discoideum with Fluorescent Antiserum. J Cell Sci 1 June 1968; 3 (2): 151–159. doi: https://doi.org/10.1242/jcs.3.2.151
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