Exposure of axenic cultures of the amoeboflagellate Naegleria gruberi EGs to the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) resulted in the induction of virus-like particles (VLP) and various structures associated with their development and presumed transmission. Previously, VLP induction could be accomplished only by growing amoebae in the presence of living bacteria as a food source. Addition of excess thymidine along with BrdU did not block induction of particles. This account demonstrates that the EGs-VLP system responded to BrdU as do a number of mammalian cell lines harbouring latent viruses, and provides the basis for future work on the infectivity of the VLPs for other amoebae as well as tissue culture cells.
Skip Nav Destination
JOURNAL ARTICLE| 01 August 1977
5-Bromodeoxyuridine-induced formation of virus-like particles in Naegleria gruberi EGs
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1977 by Company of Biologists
J Cell Sci (1977) 26 (1): 359–371.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Search Site
F.L. Schuster, J.S. Clemente; 5-Bromodeoxyuridine-induced formation of virus-like particles in Naegleria gruberi EGs. J Cell Sci 1 August 1977; 26 (1): 359–371. doi: https://doi.org/10.1242/jcs.26.1.359
Download citation file: