Nuclear sap, on fixation in glutaraldehyde, forms a fibrous network that resembles chromatin in its dimensions and staining properties. This artifactual network is easily confused with true chromatin fibres in sections of nuclei. With formaldehyde a homogeneous array of beads is produced. These are approximately 10 nm in diameter - the size of nucleosomes - and are interconnected by exceedingly fine fibrils. Each fixative and buffer imposes its own distinctive, reproducible pattern on nuclear sap. The structure of nuclear sap in life cannot be deduced from this range of patterns.
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JOURNAL ARTICLE| 01 August 1977
Chromatin-like artifacts from nuclear sap
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1977 by Company of Biologists
J Cell Sci (1977) 26 (1): 301–310.
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R.J. Skaer, S. Whytock; Chromatin-like artifacts from nuclear sap. J Cell Sci 1 August 1977; 26 (1): 301–310. doi: https://doi.org/10.1242/jcs.26.1.301
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