An apparatus is described by means of which the power versus frequency spectrum of the photomultiplier current can be obtained for laser light scattered by streaming cytoplasm in the algal cell Chara corallina. A Doppler peak is noted in the spectrum which is abolished when cytoplasmic streaming is arrested by electrical stimulation. For 5 cells of Chara, this simple laser-Doppler velocimeter gave streaming velocities (46-7 mum s-1, S.D. +/− 4–8 at 20 degrees C) similar to those obtained for the same cells using the light microscope (44-3 mum s-1, S.D. +/− 5-3 at 20 degrees C). A narrow distribution of streaming velocities is indicated. The technique described provides a rapid, quantitative assay of the in vivo rheological properties of cytoplasm.
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JOURNAL ARTICLE| 01 December 1976
Cytoplasmic streaming in Chara corallina studied by laser light scattering
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1976 by Company of Biologists
J Cell Sci (1976) 22 (3): 633–643.
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D.B. Sattelle, P.B. Buchan; Cytoplasmic streaming in Chara corallina studied by laser light scattering. J Cell Sci 1 December 1976; 22 (3): 633–643. doi: https://doi.org/10.1242/jcs.22.3.633
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