Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular. The concentration of salt present during isolation of human nucleoids affects their protein content. When made in I-95 M NaCl they lack histones and most of the proteins characteristic of chromatin; in 1-0 M NaCl they contain variable amounts of histones. The effects of various treatments on nucleoid integrity were investigated.
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JOURNAL ARTICLE| 01 November 1976
Characterization of nuclear structures containing superhelical DNA
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1976 by Company of Biologists
J Cell Sci (1976) 22 (2): 303–324.
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P.R. Cook, I.A. Brazell, E. Jost; Characterization of nuclear structures containing superhelical DNA. J Cell Sci 1 November 1976; 22 (2): 303–324. doi: https://doi.org/10.1242/jcs.22.2.303
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