The duration of the cell cycle in the blastema of regenerating limbs of axolotls has been measured by means of [3H]thymidine pulse labelling and autoradiography. A chase was required to define the pulse period. An average cell cycle at 20 degrees C takes 53 h, S-phase takes 38 h; including parts of mitosis, G1 is 10 h and G2 is 5 h long. The protracted cycle and S-phase are consonant with the large genome in axolotis and other urodeles. The rapidly growing blastema probably contains a steady population of about 5000 proliferating cells, as there is a regular withdrawal of differentiating cells from the population. The kinds of determination which exist in this population of cells, or are exerted on it, are briefly considered.
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JOURNAL ARTICLE| 01 May 1976
The cell cycle during amphibian limb regeneration
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1976 by Company of Biologists
J Cell Sci (1976) 20 (3): 539–547.
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H. Wallace, M. Maden; The cell cycle during amphibian limb regeneration. J Cell Sci 1 May 1976; 20 (3): 539–547. doi: https://doi.org/10.1242/jcs.20.3.539
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