High-resolution techniques for the localization of lectins are described. Concanavalin A (Con A) and phytohaemagglutinin (PHA) are localized using a fluorescent method with (FITC)-labelled immunoglobulins which bind to the lectins in sections of jack and red kidney bean cotyledons. Specificity is defined by the use of specific sugar inhibitors. Both Con A and PHA are found in cytoplasmic sites. Lectins with beta-glycoside specificity are detected with red-coloured artificial carbohydrate antigens. The beta-galactosyl and beta-glucosyl antigens bind specifically to clusters of spherical bodies in the intercellular spaces, to cell wall sites, and to the periphery of the cytoplasm associated with the cell membrane.
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JOURNAL ARTICLE| 01 October 1975
Localization of lectins in legume cotyledons
Online Issn: 1477-9137
Print Issn: 0021-9533
© 1975 by Company of Biologists
J Cell Sci (1975) 19 (1): 157–167.
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A.E. Clarke, R.B. Knox, M.A. Jermyn; Localization of lectins in legume cotyledons. J Cell Sci 1 October 1975; 19 (1): 157–167. doi: https://doi.org/10.1242/jcs.19.1.157
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