Extracellular cathepsin D has been demonstrated immunohistochemically in cultured chicken and rabbit cartilage. Enzyme released from viable cells was captured by specific anti-cathepsin D antibody to form extracellular immunoprecipitates which were demonstrated with fluorescein-labelled antibodies. In unfixed frozen sections these immunoprecipitates could be dissolved in antigen excess, and closely resembled those observed in immunoprecipitin lines. Extracellular cathepsin D was detected in the perichondria of normal chick cartilaginous limb-bones and, to a lesser degree, beneath them in the epiphyseal cartilage. In cultured rabbit ear cartilage, cathepsin D was detected around chondroblasts and some peripherally situated chondrocytes. In chicken and rabbit cartilage exposed to supra-physiological levels of retinol, much extracellular enzyme was detected which had been released from chondrocytes of hypertrophic and epiphyseal chick cartilage and rabbit ear cartilage.
Cathepsin D in Cartilage: The Immunohistochemical Demonstration of Extracellular Enzyme in Normal and Pathological Conditions
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A. R. POOLE, R. M. HEMBRY, J. T. DINGLE; Cathepsin D in Cartilage: The Immunohistochemical Demonstration of Extracellular Enzyme in Normal and Pathological Conditions. J Cell Sci 1 January 1974; 14 (1): 139–161. doi: https://doi.org/10.1242/jcs.14.1.139
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