First person – Samruddhi Shembekar

Samruddhi Shembekar

How would you explain the main findings of your paper in lay terms?

Mitochondria are transported by motor proteins inside a cell, and their shape and internal architecture remain intact along the movement. It is particularly interesting to study how two layers of mitochondria are held together by proteins that act as a bridge between those layers.

Were there any specific challenges associated with this project? If so, how did you overcome them?

This work involved the development of multiple knockout cell lines, which took a long time before we could start the actual experiments. Another challenge was the limited time available to finish the project, since I was the last graduate student in our lab. However, the expertise and efficiency of our technicians were pivotal to this work – cheers to Petra, Ulrike, Birgit and Karin!

When doing the research, did you have a particular result or ‘eureka’ moment that has stuck with you?

I was studying the role of metaxin 3 (Mtx3) in maintaining mitochondrial crista architecture. Biochemical experiments with this protein were particularly tricky given that it is a membrane protein, and there was very little previous research on Mtx3 to build upon. When we saw the mitochondrial structure in Mtx3-knockout cells using an electron microscope, it was a true ‘eureka’ moment for us because that was the first visual representation of what Mtx3 was doing to mitochondrial cristae. With that boost, we then developed biochemical experiments to investigate its association with MICOS proteins in cristae architecture maintenance.

Why did you choose Journal of Cell Science for your paper?

Some articles on myosin 19 have been published in Journal of Cell Science (JCS) and our work builds on those studies. Therefore, submitting to JCS made complete sense. Moreover, we have had great experiences with JCS in the past during the review process, and it has been the same way for this article as well.

Have you had any significant mentors who have helped you beyond supervision in the lab? How was their guidance special?

My supervisor Dr Martin Bähler has been exceptionally supportive throughout my time in the lab. Not only he is a great scientific mentor, but he is also a very kind person. His immense support during personal crises helped me stay active in the lab. Moreover, the other graduate students at the institute supported me in many ways – from sharing reagents and troubleshooting protocols to even helping with small experiments on weekends!

What motivated you to pursue a career in science, and what have been the most interesting moments on the path that led you to where you are now?

I participated in a biology quiz during high school, which is where I first realized my interest in science. After that, I had excellent mentors during my bachelor's and master's studies who motivated me to pursue a career in scientific research. Interestingly, the rejections and criticism I faced during the application process for PhD positions provided valuable insights about myself, and played a crucial role in leading me to where I am today.

Who are your role models in science? Why?

I would say that all my colleagues are my role models in science. Their diligence and perseverance motivate me to improve every day. Moreover, their cheerful spirit creates a nice vibe in the lab space. Throughout my PhD journey, each person around me has taught me valuable lessons and I would like to carry forward all these positive experiences.

What's next for you?

I will be joining Dr Ishier Raote's lab at Institute Jacques Monod in Paris as a postdoctoral scientist. I am excited to work on the interesting topic of cargo trafficking, as well as exploring Paris!

Tell us something interesting about yourself that wouldn't be on your CV

I have two things on my mind right now – learning how to play the piano and taking a psychology course.