ABSTRACT
First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping researchers promote themselves alongside their papers. Michelle Ly is first author on ‘ Integrin-based adhesions promote cell–cell junction and cytoskeletal remodelling to drive embryonic wound healing’, published in JCS. Michelle conducted the research described in this article while a MASc student in Rodrigo Fernandez-Gonzalez's lab at University of Toronto, Canada where she investigated the molecular mechanisms underlying cell migration and cell–extracellular matrix adhesion, and their roles in embryology and developmental processes. She is now a Research Assistant in the lab of Stewart Russel at the CReATe Fertility Centre, Toronto, Canada, working to pinpoint biomarkers associated with successful early embryo development.
Michelle Ly
How would you explain the main findings of your paper in lay terms?
Embryos have a remarkable ability to heal wounds rapidly without inflammation or scarring. This process is conserved across species and involves the cells moving together around the wound to seal the lesion. In many systems, cell migration involves adhesion to and remodelling of the extracellular matrix (ECM), a scaffold that holds cells together through integrin-based adhesions, which anchor cells to this matrix. Although previous work on embryonic wound closure focused on cell–cell adhesion and the cytoskeleton, the role of integrin-based adhesion has not been investigated. We studied the role of cell–ECM adhesion during wound healing in embryos of the fruit fly (Drosophila melanogaster). Interestingly, when the integrin gene was inactivated or when small molecules blocked integrin binding, the wound closure was delayed. Our results suggest that cell–ECM adhesion contributes to rapid wound repair and reveal a previously unrecognized interplay between cell–cell and cell–ECM adhesion in the collective cell movements that drive embryonic wound closure.
Have you had any significant mentors who have helped you beyond supervision in the lab? How was their guidance special?
Two mentors who played a significant role in my graduate school experience were Rodrigo Fernandez-Gonzalez, my PI, and Katy Rothenberg, a post doc that I worked with closely. Rodrigo always pushed me to develop my confidence and get out of my shell. For example, I used to get nervous with public speaking, so Rodrigo encouraged me to practice speaking at conferences, seminars and lab meetings. Throughout my time in his lab, he gave me lots of feedback, which helped me to be more confident in myself. Meanwhile, Katy's consistent support, insights and genuine interest in my growth as a scientist went far beyond our research work together. She was an invaluable source of inspiration and encouragement, and was always ready to help whether it was in my research or career development. She would even give moral support when my fear started to kick in during rock climbing!
Embryonic wound healing involves cell–ECM adhesion remodelling. The Drosophila epidermis (stage 14, 10–12 h post-fertilization) in embryos expressing GFP-tagged Laminin A, and mCherry-tagged Serpent, stained with antibodies against GFP (gray) and mCherry (magenta), and with phalloidin to visualize F-actin (green).
Embryonic wound healing involves cell–ECM adhesion remodelling. The Drosophila epidermis (stage 14, 10–12 h post-fertilization) in embryos expressing GFP-tagged Laminin A, and mCherry-tagged Serpent, stained with antibodies against GFP (gray) and mCherry (magenta), and with phalloidin to visualize F-actin (green).
What motivated you to pursue a career in science, and what have been the most interesting moments on the path that led you to where you are now?
It all began during my undergraduate years, when I first caught a glimpse of the fascinating world of research. As I delved deeper into my studies, I discovered a profound passion for biology, particularly with an extraordinary, resilient and microscopic animal called the tardigrade. Exploring their incredible adaptations and survival in the harshest of environments and irradiation made me realize how vast the world of research is. From this, I had a new-found drive to understand the underlying mechanisms of nature. As I transitioned into pursuing a master's degree, I knew I wanted to explore more of the cellular and molecular mechanism of development. By using Drosophila embryonic wound closure as my model, and fluorescent microscopy, I was able to deepen my understanding of how cells coordinate together to repair tissues. These experiences not only deepened my understanding of the scientific process, but also solidified my commitment to unraveling the complexities of biological systems.
What's next for you?
I recently started a new job as a research assistant with the Embryology and Implantation team at CReATe Fertility Centre. I am excited be studying developmental biology in a new system: the human! I was driven by the desire to advance my career, explore diverse opportunities and broaden my skillset. This move also offers practical experience and the chance to explore new aspects of research, as well as opening the door to a dynamic and rewarding career in the field of development.
Tell us something interesting about yourself that wouldn't be on your CV
I've always had a love for board games, specifically puzzle or strategy-based games. My current favourites are Catan, Genius Squares and the Exit game series. Perhaps my passion for research stems from my love for puzzles, as every experiment and discovery bring me one step closer to unravelling the bigger picture!
Michelle Ly’s contact details: [email protected]
