First person – Yuejia Li

Yuejia Li

How would you explain the main findings of your paper in lay terms?

Imagine microtubules as tiny, strong cables inside a cell. They are part of the cell's skeleton, which helps the cell maintain its shape and structure. These cables are very important because they help the cell move and transport materials from one area to another, much like how a highway system helps transport goods within a city. The stability and dynamics of microtubules are crucial for the cell's health and function. Microtubule dynamics refer to the constant assembly and disassembly of these cables. Microtubule stability is important because it ensures that these cables can perform their tasks without breaking down, much like how a strong bridge is necessary for the safe flow of traffic. In our research, we elucidated the molecular mechanism of how a protein called CAMSAP3 mediates microtubule stabilization. Additionally, our research explores the unique functional differences among CAMSAP family members and uncovered how these differences influence their subcellular localization and regulation of microtubule minus end stability. By investigating these molecular mechanisms, we provide a clearer understanding of how CAMSAP proteins contribute to microtubule dynamics.

Were there any specific challenges associated with this project? If so, how did you overcome them?

We discovered that the α-helix can mediate the dimerization of CAMSAP3, but the connection between this and its function in stabilizing microtubules was initially unclear to us. Under the guidance of my mentor, we devised a strategy to induce artificial dimerization to test our hypothesis. The outcomes were in line with our predictions, demonstrating that dimerization of the CKK domain of CAMSAP3 indeed contributes to the stabilization of microtubules.

When doing the research, did you have a particular result or ‘eureka’ moment that has stuck with you?

In our experiments, upon expressing two CKK domain monomers within cells and triggering their dimerization with rapamycin to examine their distribution, we were struck with amazement as the originally dispersed CKK rapidly assumed a pattern aligning with microtubules, all within ∼5 s post rapamycin addition. This swift transformation marked a defining ‘eureka’ moment in our research.

Why did you choose Journal of Cell Science for your paper?

Journal of Cell Science’s focus on cell biology perfectly aligns with our research, which explores novel mechanisms in microtubule stabilization. Additionally, the Journal's reputation for timely publication aligns with our desire to share our results as quickly as possible. We have had positive experiences with Journal of Cell Science in the past, which further solidified our decision to submit our paper there.

Have you had any significant mentors who have helped you beyond supervision in the lab? How was their guidance special?

Throughout my academic journey, I've been fortunate to encounter several mentors, but one who stands out is my principal investigator, Dr Wenxiang Meng. He is passionate about science and provides very meticulous guidance to students. I am particularly thankful for the trust and encouragement he has extended to me, which has been instrumental in building my confidence to address complex scientific challenges. His influence has shaped me into the researcher and individual I am today. I feel a profound sense of pride in embodying the lessons I've learned from his guidance and am committed to applying them in my future endeavors.

What motivated you to pursue a career in science, and what have been the most interesting moments on the path that led you to where you are now?

During my middle school years, I was captivated by the ‘magical’ images and phenomena presented in our natural science textbooks, which sparked a deep curiosity in me to unravel the mysteries they held. Additionally, there was a shared sentiment among me and my peers that becoming a scientist was the most exhilarating career one could aspire to.

Who are your role models in science? Why?

My role models in science are those who have achieved remarkable success in their respective fields and concurrently have skillfully navigated the unique challenges faced by women in science, all while sustaining a rich and fulfilling personal life. Their examples serve as a beacon, motivating me to pursue my scientific ambitions with vigor, while also striving to maintain a harmonious balance and richness in my life beyond the laboratory.

What's next for you?

I am in the process of expeditiously finalizing my application for a doctoral degree, with the intention of subsequently pursuing a postdoctoral role to delve deeper into the enigmatic realms of natural science.

Tell us something interesting about yourself that wouldn't be on your CV

I have a passion for culinary adventures, which extends beyond simply seeking out exquisite dining spots. I take great pleasure in preparing meals at home and sharing these culinary creations with loved ones, finding the process to be both therapeutic and heartwarming.