This Correction replaces and updates a previous Correction to J. Cell Sci. (2020) 133, jcs249862 ().
In Fig. S2, four bimolecular fluorescence microscopy (BiFC) confocal microscope images were inadvertently duplicated. These panels, which all contain only control background fluorescence, were not used to infer any positive interactions and the errors do not affect the results or conclusions of the paper.
The correct figure is shown below and the online supplementary material has been updated.
The authors apologise for these errors and any inconvenience caused.
Control bimolecular fluorescence complementation (BiFC) assays involving PBAC1-5. (A) Pairwise expression of the PBAC3 and PBAC4 chaperones with themselves and PBAC1, PBAC2 and PBAC5 indicates that PBAC3 and PBAC4 interact in planta. (B) Pairwise expression of the PBAC1-5 chaperones fused to the N-terminal (NY) and C-terminal (CY) halves of YFP by themselves indicate that only the NY-PBAC4 construct produces a fluorescence signal due to auto-activation. Nicotiana benthamiana leaf epidermal cells were co-infiltrated with the indicated plasmid combinations, and fluorescence signals were detected by confocal fluorescence microscopy 36 h after infiltration. Shown are the fluorescence images alone or merged with their companion bright field images. Scale bar=20 μm.
Control bimolecular fluorescence complementation (BiFC) assays involving PBAC1-5. (A) Pairwise expression of the PBAC3 and PBAC4 chaperones with themselves and PBAC1, PBAC2 and PBAC5 indicates that PBAC3 and PBAC4 interact in planta. (B) Pairwise expression of the PBAC1-5 chaperones fused to the N-terminal (NY) and C-terminal (CY) halves of YFP by themselves indicate that only the NY-PBAC4 construct produces a fluorescence signal due to auto-activation. Nicotiana benthamiana leaf epidermal cells were co-infiltrated with the indicated plasmid combinations, and fluorescence signals were detected by confocal fluorescence microscopy 36 h after infiltration. Shown are the fluorescence images alone or merged with their companion bright field images. Scale bar=20 μm.
Control bimolecular fluorescence complementation (BiFC) assays involving PBAC1-5. (A) Pairwise expression of the PBAC3 and PBAC4 chaperones with themselves and PBAC1, PBAC2 and PBAC5 indicates that PBAC3 and PBAC4 interact in planta. (B) Pairwise expression of the PBAC1-5 chaperones fused to the N-terminal (NY) and C-terminal (CY) halves of YFP by themselves indicate that only the NY-PBAC4 construct produces a fluorescence signal due to auto-activation. Nicotiana benthamiana leaf epidermal cells were co-infiltrated with the indicated plasmid combinations, and fluorescence signals were detected by confocal fluorescence microscopy 36 h after infiltration. Shown are the fluorescence images alone or merged with their companion bright field images. Scale bar=20 μm. Duplicated panels are indicated in red boxes.
Control bimolecular fluorescence complementation (BiFC) assays involving PBAC1-5. (A) Pairwise expression of the PBAC3 and PBAC4 chaperones with themselves and PBAC1, PBAC2 and PBAC5 indicates that PBAC3 and PBAC4 interact in planta. (B) Pairwise expression of the PBAC1-5 chaperones fused to the N-terminal (NY) and C-terminal (CY) halves of YFP by themselves indicate that only the NY-PBAC4 construct produces a fluorescence signal due to auto-activation. Nicotiana benthamiana leaf epidermal cells were co-infiltrated with the indicated plasmid combinations, and fluorescence signals were detected by confocal fluorescence microscopy 36 h after infiltration. Shown are the fluorescence images alone or merged with their companion bright field images. Scale bar=20 μm. Duplicated panels are indicated in red boxes.
