Hook1 is a coiled-coil protein that has a role in tethering microtubules and cargo during clathrin-independent endocytosis. Hook1 is involved in the sorting of cargo proteins, such as CD98 and CD147, into recycling endosomes, which ensures that these proteins avoid transport to lysosomes for degradation. It is unclear, however, how endocytosed Hook1-mediated cargo proteins are recycled back to the plasma membrane. Now, Hiromichi Shirataki and colleagues (Higashi et al. 2022) identify the protein γ-taxilin as an important negative regulator of Hook1. Knowing that α-taxilin has a role in intracellular vesicle trafficking, the authors postulate that another member of the family, γ-taxilin, might have a similar role. Using yeast two-hybrid screening, they identify Hook1 as a novel binding partner of γ-taxilin and show that γ-taxilin binds to Hook1 competitively with CD98. Depletion of γ-taxilin promotes the formation of CD98- or CD147-containing tubular recycling endosomes and a faster rate of recycling of these cargo proteins back to the plasma membrane; depletion also promotes CD147-mediated cell spreading. Overexpression of γ-taxilin, however, inhibits CD98-positive tubular endosome formation. Finally, the authors demonstrate that γ-taxilin competes with CD98 and CD147 for binding to the C-terminal region of Hook1. Based on these findings, the authors suggest that γ-taxilin negatively regulates the sorting into tubular endosomes of Hook1-mediated cargo proteins by inhibiting the interaction between them and Hook1. These data make an important contribution to the mechanistic understanding of endosomal sorting of cargo proteins.
γ-Taxilin controls sorting of clathrin-independent cargo
- Split-screen
- Views Icon Views
-
Article Versions Icon
Versions
- Version of Record 12 January 2022
- Share Icon Share
-
Tools Icon
Tools
- Search Site
γ-Taxilin controls sorting of clathrin-independent cargo. J Cell Sci 1 January 2022; 135 (1): e135_e0104. doi:
Download citation file:
Advertisement
Cited by
Special Issue: Cell Biology of Lipids
-LipidSI.png?versionId=3647)
Lipids are the main component of cellular membranes, but have key roles in many essential cellular processes, as well as structural and signalling functions. Our 2022 special issue showcases the diversity of current research in lipid cell biology.
The special issue includes an interview with Guest Editor James Olzmann. James has also contributed to this lipid droplet wall poster from our sponsor, Cayman Chemical.
Propose a new Workshop
-GSWorkshop.png?versionId=3647)
Our Workshops bring together leading experts and early-career researchers from a range of scientific backgrounds. Applications are now open to propose Workshops for 2024, one of which will be held in a Global South country.
We’re hiring: Community Manager, preLights
-preLights.jpg?versionId=3647)
We are seeking to appoint a new Community Manager to run our preprint highlighting service, preLights. Launched in 2018, this initiative has gained significant attention from researchers and the publishing industry. We are now looking for the right person to join us for the next phase of community building and the site’s growth and development. Please see the full job description for further details.
The application deadline is 20 May 2022.
Essay series: Equity, diversity and inclusion in cell biology
-EssaySeries.png?versionId=3647)
The JCS Essay Series is an initiative to help showcase and provide a platform for voices in the field of cell biology. The first topic we covered was 'Equity, diversity and inclusion in cell biology', and the winning and runner up essays are now available to read.
FocalPlane Network launched
-FocalPlaneNetworkLaunch.png?versionId=3647)
We are excited to announce the launch of the FocalPlane Network, an international directory of microscopists. The idea behind the FocalPlane Network is to facilitate promotion and networking as well as assist those seeking conference speakers, committee members, reviewers or collaborators. We hope that it will help promote diversity in the community. Find out more and join the Network here.