Staufen1 (STAU1) is a double-stranded RNA-binding protein involved in the post-transcriptional regulation of gene expression that affects several different cellular processes, such as mitosis. The expression levels of STAU1 have been shown to vary during the cell cycle, but its importance for the progression of mitosis is still poorly understood. In this study, Luc DesGroseillers and colleagues (Hassine et al., 2020) demonstrate that STAU1 associates with and mediates the localisation of a subset of RNAs to the mitotic spindle. The authors observed that the STAU155 isoform, but not STAU163, colocalises with α-tubulin on the mitotic spindle of colorectal cancer HCT116 cells and non-transformed hTERT-RPE1 cells; this is dependent on the first 88 N-terminal amino acids in STAU1, which are not involved in RNA binding. An analysis of purified mitotic spindles, compared to cell extracts, shows an enrichment of different types of RNA, including mRNAs and precursor ribosomal RNA (pre-rRNA). Moreover, purified spindles from STAU1-knockdown cells exhibit decreased levels of some of these mRNAs and pre-rRNA. Finally, the ribosomal protein S6 and OP-puromycin, which marks active translation sites, colocalises with STAU1 along the mitotic spindle, suggesting that STAU1 might be involved in these spindle-associated translation events. Taken together, these results uncover a new role for STAU1 in the regulation of RNA localisation and post-transcriptional pathways during mitosis.