Neuronal guidance and extension is mediated by the growth cone at the tip of the growing axon. Directionality and motility of the growth cone are orchestrated by both microtubule (MT) and F-actin cytoskeletal dynamics. Interplay and coordination between the cytoskeletal elements is required for the movement of the axon and its response to guidance cues, but the molecular mechanisms for this crosstalk are still largely unknown. Now, Laura Anne Lowery and co-workers (Slater et al., 2019) investigate the role of the MT polymerase Xenopus microtubule associated protein 215 (XMAP215) in the growth cone for axonal growth. The authors find that XMAP215 contributes to the interaction between MTs and actin in the growth cone, and the alignment of both cytoskeletal networks is specifically mediated by TOG domains 1–5 in the N-terminus. In addition, XMAP215 colocalizes with F-actin in the periphery of the growth cone. Furthermore, the authors use in vitro assays to demonstrate binding between F-actin and XMAP215. Depletion of XMAP215 leads to defects in growth cone pausing events, growth cone morphology changes and an inability to respond to the guidance cue factor ephrin A5. This work proposes a role for XMAP215 in the coordination of actin and MT cytoskeletons and for proper growth cone morphology in growing axons.