Skin melanocytes are cells that synthesise pigment and transport it, via organelles termed melanosomes, into their dendrites for subsequent distribution to neighbouring keratinocytes, which is the basis of pigmentation in mammalian skin and hair. However, it is unclear which proteins are involved in this centrifugal transport of melanosomes; some studies suggest that it is mediated by myosin-Va on actin filaments, and others that it is mediated by kinesin-1 on microtubules. Here (p. 2056), Alistair Hume and colleagues set out to resolve this controversy. They find that neither Kif5b, the most abundant kinesin-1 heavy chain in melanocytes, nor its targeting factor Rab1a, show an obvious association with melanosomes. Moreover, neither siRNA-mediated knockdown of Kif5b nor expression of dominant-negative Rab1a affect the cellular distribution of melanosomes, whereas knockdown of Rab27a, which activates myosin-Va, prevents their centrifugal transport. However, constructs of Kif5b that are artificially targeted to melanosomes, by replacing the endogenous Kif5b cargo-binding tail with that from other melanosome-binding proteins, can drive the centrifugal movement of melanosomes in myosin-Va-deficient cells. This suggests that Kif5b is not involved in melanosome transport because it is recruited to these organelles inefficiently. Overall, these results show that long-distance centrifugal organelle transport is mediated by the actin network in this system.