Investigations on the morphological features and some biochemical properties of cells isolated from mouse C3H/He (IRE/Ar) submaxillary glands cultivated in vitro are reported.
Two types of cells have been recognized: the dark cells differ from the light in the shape of their mitochondria, and number of vacuoles, while endoplasmic reticulum is absent.
The enzymic activities, which have been tested, are phosphofructokinase, pyruvate kinase, amylase and lactate dehydrogenase. The values for enzyme activities in vitro are in good agreement with those obtained for submaxillary gland in vivo.
Therefore, it can be concluded that during their growth in vitro cells from submaxillary glands retain their enzymic properties.