Type VII collagen is the main component of anchoring fibrils, structures that are integral to basement membrane homeostasis in skin. Mutations in the gene encoding type VII collagen COL7A1 cause recessive dystrophic epidermolysis bullosa (RDEB) an inherited skin blistering condition complicated by frequent aggressive cutaneous squamous cell carcinoma (cSCC). OATP1B3, which is encoded by the gene SLCO1B3, is a member of the OATP (organic anion transporting polypeptide) superfamily responsible for transporting a wide range of endogenous and xenobiotic compounds. OATP1B3 expression is limited to the liver in healthy tissues, but is frequently detected in multiple cancer types and is reported to be associated with differing clinical outcome. The mechanism and functional significance of tumour-specific expression of OATP1B3 has yet to be determined. Here, we identify SLCO1B3 expression in tumour keratinocytes isolated from RDEB and UV-induced cSCC and demonstrate that SLCO1B3 expression and promoter activity are modulated by type VII collagen. We show that reduction of SLCO1B3 expression upon expression of full-length type VII collagen in RDEB cSCC coincides with acquisition of front-to-rear polarity and increased organisation of 3D spheroid cultures. In addition, we show that type VII collagen positively regulates the abundance of markers implicated in cellular polarity, namely ELMO2, PAR3, E-cadherin, B-catenin, ITGA6 and Ln332.
Incidence of non-melanoma skin cancer (NMSC) is highest among all malignancies in Caucasian populations (Toms, 2004; Cancer Research UK, 2012, http://publications.cancerresearchuk.org/cancerstats/statsskin/keyfactsskin.html). Approximately 80% of NMSCs comprise basal cell carcinoma (BCC) and 20% cutaneous squamous cell carcinoma (cSCC) (Alam and Ratner, 2001; Yanofsky et al., 2011). BCC is usually a benign disease and is rarely responsible for skin-cancer-related mortality, whereas cSCC can develop into metastatic disease and contributes to around 1 in 4 skin cancer deaths in the UK (ISD Scotland, http://www.isdscotland.org/isd/183.html). The main risk factor for cSCC in the general population is UV radiation (Fears and Scotto, 1983; Preston and Stern, 1992), but other factors such as HPV infection (McGregor and Proby, 1996; Harwood and Proby, 2002) and immunosuppression in organ transplant patients (Rowe et al., 1992; Veness et al., 1999) have been documented. Inherited skin disease can also predispose to developing cSCC (Ng et al., 2011); in particular, individuals diagnosed with recessive dystrophic epidermolysis bullosa (RDEB) (Reed et al., 1975; Fine et al., 2009). Patients with this disease are affected by deleterious mutations in COL7A1, leading to either defective or absent type VII collagen (Christiano et al., 1993). Type VII collagen is the main component of anchoring fibrils, structures which associate with the basement membrane and are essential for the proper attachment of the dermis to the epidermis (Sakai et al., 1986; Burgeson, 1993). Type VII collagen interacts with proteins localised close to the basement membrane; laminin 332 (Ln332) and type IV collagen (Chen et al., 1997; Rousselle et al., 1997), as well banded collagen I that are localised in the dermis (Villone et al., 2008), providing strong adhesive connection between these two compartments. Over 90% of RDEB patients develop metastatic cSCC by age 55, a figure significantly higher than that in the general population where metastatic disease is relatively rare (Weinstock et al., 1991; Miller and Weinstock, 1994; Fine et al., 2009). The underlying molecular mechanism driving predisposition to develop aggressive cSCC in RDEB is not fully understood (South and O'Toole, 2010) although recent data from our laboratory show that tumour microenvironment, in particular the altered composition of extracellular matrix in RDEB, can promote tumours (Ng et al., 2012). Similarly, despite an alarming rise in the incidence of cSCC in the general population and a relatively low 5-year survival in patients presenting with regional metastasis (Rowe et al., 1992; Czarnecki et al., 1994), little is understood about these tumours with poor prognosis.
We previously identified a few differentially expressed genes (DEGs) using microarray to compare RDEB with UV-induced cSCC in a relatively small number of keratinocyte cultures (n = 9) (Watt et al., 2011). We further investigate those 18 DEGs and identify SLCO1B3, a gene that encodes the liver-specific organic anion transporter polypeptide OATP1B3, as being upregulated in both UV-induced and RDEB cSCC when compared with normal skin. Transporter genes are biologically significant for cell homeostasis and can regulate cellular mechanisms such as metabolism, cell death and motility (Olk et al., 2009; Klaassen and Aleksunes, 2010). OATP1B3 is normally expressed in the liver and is involved in the transport of bile salts, glutathione and other organic anions (Smith et al., 2005a; Hagenbuch and Gui, 2008). In agreement with our data, OATP1B3/SLCO1B3 is frequently upregulated in solid tumours including breast and colon (Lockhart et al., 2008; Maeda et al., 2010; Pressler et al., 2011) and it has been suggested that OATP1B3 has therapeutic potential owing to its ability to transport a broad range of drugs, including methotrexate, paclitaxel, docetaxel, bromsulphalein (BSP) and pitavastatin (Smith et al., 2005b; Hagenbuch and Gui, 2008). OATP1B3 expression has been reported to associate with clinical outcome in a number of studies (Hamada et al., 2008; Lockhart et al., 2008) yet little data exist on the mechanism of expression or functional consequence. Lee and colleagues report that OATP1B3 promotes apoptotic resistance in tumour cells (Lee et al., 2008) and very recently, hypoxia has been shown to positively regulate OATP1B3 expression (Ramachandran et al., 2013).
Here, we investigate the relationship between type VII collagen and tumour expression of SLCO1B3 and demonstrate that type VII collagen, Ln332 and ELMO2 regulate tumour expression of SLCO1B3. We further show that expression of type VII collagen in RDEB cSCC keratinocytes promotes front-to-rear polarity on uncoated plastic and increases organisation of 3D spheroid cultures. These structural changes in response to expression of type VII collagen coincide with an increase in the abundance of markers implicated in cellular polarity, namely ELMO2, PAR3, E-cadherin, B-catenin, ITGA6 and Ln332.
SLCO1B3 is expressed in cSCC in vitro and in vivo
We previously identified only 18 genes to be significantly differentially expressed between cultured RDEB cSCC keratinocytes and UV-induced cSCC keratinocytes using gene expression arrays, demonstrating these two tumour groups are not significantly different in this assay (Watt et al., 2011). Initial follow-up of these 18 genes comparing three RDEB cSCC and three UV cSCC cultures using sybr®green qPCR revealed that 12 genes showed the same trend as array data but only five were significantly dysregulated: SLCO1B3, DUSP23, TJAP1, SNRPN and SNURF (data not shown). From these five genes, SLCO1B3 showed a consistent upregulation in RDEB cSCC keratinocytes in culture compared with normal primary keratinocytes and the majority of UV-induced cSCC keratinocytes, with the exception of SCCT8 (Fig. 1A). However this experiment also identified a significant increase in expression in the majority of UV-induced cSCC keratinocytes when compared with normal keratinocytes. Six of eight cultured cSCC cell lines showed a greater level of expression compared with the hepatocellular carcinoma cell line HepG2 (Fig. 1A).
Q-PCR using reverse transcribed mRNA obtained from patient tissues showed high SLCO1B3 expression in RDEB cSCC, and to a lesser extent UV-induced cSCC and RDEB non-SCC skin compared with normal skin, with the highest levels evident in RDEB cSCC tissue (Fig. 1B).
Endogenous OATP1B3 expression is detected in cSCC keratinocytes but only localises to the membrane in vivo
OATP1B3 expression was readily detected in cSCC keratinocytes but not in normal primary keratinocytes in culture (Fig. 2A). Plasma-membrane-bound OATP1B3 was, however, not observed in cultured cSCC keratinocytes using indirect immunofluorescence unless overexpressed by retroviral transduction (Fig. 2B). Membrane-bound protein was detected in organotypic 3D cultures (data not shown) and xenograft tumours (Fig. 2B, right).
Tissue sections obtained from RDEB cSCC (n = 7), RDEB skin (n = 3) and UV-induced cSCC (n = 6) patients along with normal human liver (positive control) and normal human skin (negative control) were assessed for OATP1B3 expression. Samples from 6/7 RDEB cSCC and 5/6 cSCC showed heterogeneous plasma-membrane-bound protein expression along with nuclear localisation in 3/7 RDEB cSCC and 2/6 cSCC tissues (Fig. 3A, top and Fig. 3B). Membrane-bound protein was found in 1/3 RDEB skin samples (Fig. 3A, bottom). Colocalisation with plakoglobin confirmed membrane localisation, although higher levels of OATP1B3 were readily detected in regions of cSCC tissue where membrane-bound plakoglobin was absent (Fig. 3B).
SLCO1B3 expression is regulated by type VII collagen
We noted that when wild-type COL7A1 was expressed in RDEB cSCC keratinocytes (SCCRDEB-C7 cells) the level of SLCO1B3 expression was reduced compared with empty vector control cells (SCCRDEB-EV cells) (P = 0.004, Fig. 4A). This observation was confirmed using separate clonal populations of transduced cells, ruling out any insertion effects (Fig. 4A–C). To determine whether type VII collagen had a direct effect on the promoter activity of SLCO1B3, we cloned the SLCO1B3 promoter, previously described by Jung and co-workers (Jung et al., 2002) and transfected a SLCO1B3 promoter-driven luciferase construct into either SCCRDEB3-C7 or SCCRDEB3-EV cells. SLCO1B3 promoter activity was significantly (P = 0.02) reduced in SCCRDEB3 keratinocytes transduced with collagen VII compared with control cells (Fig. 4D).
Furthermore, SLCO1B3 expression was significantly reduced in two separate patient-derived parental populations of RDEB SCC cells, SCCRDEB3 and SCCRDEB2, when cultured on matrix derived from SCCRDEB3-C7 cells compared with matrix derived from SCCRDEB3-EV vector control cells (Fig. 4E).
We then used siRNA-mediated depletion of COL7A1 to show that reduction of recombinant type VII collagen increases SLCO1B3 expression in a clonal population of SCCRDEB3-C7 cells (Fig. 5A,B). We note that in this experiment the NT control level of SLCO1B3 was greater in SCCRDEB3-EV cells than SCCRDEB3-C7 cells as expected (1.5-fold), yet the impact of siRNA-mediated depletion of COL7A1 resulted in a greater increase in SLCO1B3 levels than seen in the EV population (Fig. 5B). We attribute this observation to the clonal nature of the two cell populations. To confirm the observation that siRNA depletion of COL7A1 increased SLCO1B3 expression in non-clonal populations we next used siRNA depletion of COL7A1 in SCCRDEB4-C7 cells (Fig. 5C) and the UV-induced nonRDEB cSCC keratinocyte population SCCT8 (Fig. 5D), providing a direct link to type VII collagen and SLCO1B3 tumour keratinocyte expression. The same effect was also observed in normal primary keratinocytes (NHK) following knockdown of type VII collagen, although the overall level of expression was significantly lower than in tumour cells (supplementary material Fig. S1).
Expression of the polarity related protein ELMO2 is regulated by type VII collagen
OATP1B3 is a transmembrane transporter that is expressed in the basolateral component of the plasma membrane. Type VII collagen is a basement membrane protein that directly binds Ln332, which itself binds integrin receptors. It has been previously reported that expression of cell surface proteins can be regulated by interactions with basement membrane components that influence cellular polarity (Li et al., 2003; Daley et al., 2012). We therefore postulated that although OATP1B3 was not observed to be asymmetrically localised in tumour keratinocytes adjacent to extracellular matrix (Fig. 3), the absence of type VII collagen might regulate its expression by altering cellular polarity.
Other than basement-membrane-associated proteins in basal keratinocytes or tight junction components in upper stratified keratinocytes, asymmetrically distributed apical basal polarity markers have yet to be defined in the epidermis (Niessen et al., 2012). Better described is the redistribution of proteins involved in the assembly and disassembly of focal adhesions during the acquisition of front-to-rear polarity in cultured single cells (Hamill et al., 2009; Ho and Dagnino, 2012b). Front-to-rear polarity in cultured primary murine keratinocytes in response to Ln332 is mediated in part by a focal adhesion complex containing the protein engulfment and motility 2 (ELMO2) (Ho et al., 2009; Ho and Dagnino, 2012b). We therefore investigated whether a relationship existed between ELMO2, type VII collagen and OATP1B3.
Overexpression of type VII collagen resulted in upregulation of ELMO2 expression in SCCRDEB3-C7 cells (Fig. 6A). Concurrently, siRNA-mediated depletion of COL7A1 in four independent tumor cell populations resulted in a reduction in ELMO2 expression; SCCRDEB3-C7 and SCCRDEB4-C7 cells as well as cSCC cells that endogenously express type VII collagen (SCCT8 and SCCT1) (Fig. 6B). ELMO2 protein levels corresponded with a reduction in transcript (Fig. 6C,D). Furthermore, ELMO2 expression increased in both SCCRDEB3 and SCCRDEB2 keratinocytes that were cultured on matrix derived from SCCRDEB3-C7 cells (Fig. 6E,F). These data demonstrate that type VII collagen positively regulates ELMO2 expression in cSCC keratinocytes.
We next investigated the link between ELMO2 and SLCO1B3 expression and showed that overexpression of ELMO2 resulted in a reduction in SLCO1B3 mRNA levels in SCCT8 and SCCRDEB2 (Fig. 7A,B), whereas a reduction in ELMO2 mRNA resulted in an increase in SLCO1B3 expression in SCCT8 (Fig. 7C,D) and non-SCC RDEB keratinocytes (supplementary material Fig. S2A–C), with a significant increase in SLCO1B3 observed in normal primary keratinocytes after ELMO2 reduction (supplementary material Fig. S2D–F), thereby demonstrating that similar to COL7A1, ELMO2 can also negatively regulate SLCO1B3.
Type VII collagen can regulate SLCO1B3 independent of ELMO2
To confirm whether expression of SLCO1B3 in cSCC was dependent on expression of either type VII collagen or ELMO2 alone, we investigated SLCO1B3 expression following knockdown of ELMO2 in RDEB cSCC cells overexpressing type VII collagen (RDEBSCC3-C7) and control cells (RDEBSCC3-EV). SLCO1B3 expression significantly increased in RDEBSCC3-EV cells following ELMO2 knockdown, demonstrating that ELMO2 can regulate SLCO1B3 in the absence of type VII collagen (supplementary material Fig. S3A,B). In RDEBSCC3-C7 cells, no significant difference in SLCO1B3 expression was observed following ELMO2 knockdown (supplementary material Fig. 3A,B), showing that depletion of ELMO2 had no effect on SLCO1B3 expression. In addition, we show that ELMO2 does not regulate type VII collagen, as observed in SCCT8 (supplementary material Fig. 3C–E) or normal primary keratinocytes (supplementary material Fig. S2A–C) following knockdown of ELMO2. We also investigated Ln332, a binding partner of type VII collagen, which is also known to regulate polarity in epithelial cells (Kligys et al., 2007). In RDEB cSCC keratinocytes, siRNA-mediated knockdown of Ln332 resulted in an increase in SLCO1B3 message levels (Fig. 7E,F). This effect was independent of ELMO2 expression (supplementary material Fig. S3F). Collectively, these data show that SLCO1B3 is regulated independently by proteins with known roles in epidermal polarity (ELMO2 and Ln332) and that SLCO1B3 expression is controlled through a single mechanism governed by either of these proteins or type VII collagen alone.
Type VII collagen regulates front-to-rear keratinocyte polarity in 2D culture and regulates structural organisation in 3D spheroid culture
Ln332 and ELMO2 have previously been shown to play a role in epidermal polarity (Li et al., 2003; Ho and Dagnino, 2012b). To investigate whether type VII collagen could also regulate keratinocyte front-to-rear polarity, we seeded RDEBSCC C7 cells at low density and assessed lamellipodia formation at 5 hours as described previously (Hamill et al., 2009) (Fig. 8A). Type VII collagen expression marginally increased the number of attached cells that spread onto uncoated plastic (Fig. 8B) but strikingly increased the number of lamellipodia observed (Fig. 8C). Cells displaying front-to-rear polarity were frequently observed in RDEBSCC C7 cells, but were completely absent in vector control cells after 5 hours (Fig. 8A). The same experiment was repeated in RDEBSCC3 cells following transient overexpression of ELMO2 to confirm the role of ELMO2 in RDEB cSCC polarity. ELMO2-overexpressing cells (ELMO2) showed a significant increase in the number of polarised cells compared with control cells (EV) (supplementary material Fig. S4).
To investigate whether type VII collagen can influence structural organisation, 3D spheroid cultures were established with SCCRDEB3-C7 cells and compared with the SCCRDEB3-EV. SCCRDEB3-C7 spheroids showed increased structural organisation determined by morphology (Fig. 8D) and overall increased levels of proteins previously associated with cellular polarity, namely PAR3, ELMO2, integrin alpha 6 and Ln332 when compared with SCCRDEB3-EV spheroids (Fig. 8D,E). Next, we assessed the effect of stromal-derived type VII collagen on RDEB SCC cells. To do this, we co-cultured parental SCCRDEB3 cells with fibroblasts either expressing recombinant type VII collagen or vector control in a spheroid assay over 7 days. After this time we observed that mesenchyme delivery of type VII collagen had a more profound effect on RDEBSCC keratinocytes in spheroid culture than on RDEBSCC keratinocytes cultured alone in the presence or absence of type VII collagen. Spheroid cultures of RDEBSCC cells with fibroblast-delivered type VII collagen exhibited more distinct separation of cellular populations and a clear increase in distribution to the membrane of β-catenin and E-cadherin (Fig. 8F). Western blot analyses showed no overall changes in E-cadherin and β-catenin protein levels (Fig. 8G), suggesting that the differences observed in solvent-fixed embedded spheroids (Fig. 8F) are a result of membrane localisation.
cSCC is the most common cause of NMSC mortality and represents a significant clinical complication in high risk groups, particularly patients with RDEB. UV exposure resulting in DNA damage is a well-established driver of cSCC (Armstrong and Kricker, 2001). By contrast, RDEB-associated cSCC disease aggression is regarded as UV independent, and although tumour initiation is still not fully understood (Fine et al., 2009), recent work from our laboratory demonstrates the role of microenvironment in RDEB tumour progression (Ng et al., 2012). Here, our study began by interrogating a small number of changes identified through expression array analysis comparing UV cSCC keratinocytes with RDEB cSCC keratinocytes in culture. In agreement with multiple previous studies, few differences were confirmed between these two tumour types (Arbiser et al., 2004; Mallipeddi et al., 2004). In fact, our primary target, SLCO1B3 is aberrantly expressed in a panel of cSCC from both UV induced and RDEB induced cSCC cell lines and tissue (Fig. 1), again in agreement with previous studies identifying SLCO1B3 expression in cancer (Maeda et al., 2010; Pressler et al., 2011; Svoboda et al., 2011). However, by pursuing the molecule responsible for RDEB we show that type VII collagen regulates the expression of SLCO1B3 in both RDEB cSCC and UV-induced cSCC (Fig. 3). Further to this, we demonstrate that front-to-rear polarity and organisation of 3D spheroid culture coincides with changes in expression of SLCO1B3 in cSCC keratinocytes when type VII collagen levels are altered (Fig. 8).
Polarity is essential for the maintenance of tissue homeostasis and can either be defined as the distribution of apical and basal membrane domains within the cell or the positioning of a cell within the tissue itself, referred to as planar cell polarity (PCP). Directional transport, asymmetric stem cell division, oriented cell division and sensing signals from the neighbouring microenvironment are a few examples of cellular processes that require the maintenance of cell and tissue polarity (Lechler and Fuchs, 2005; Martin-Belmonte and Mostov, 2008; Poulson and Lechler, 2010; Martin-Belmonte and Perez-Moreno, 2012). Previously, three main protein complexes have been implicated in maintenance of polarity in simple epithelia, Par6–Par3–aPKC (Munro, 2006; Goldstein and Macara, 2007), the Crumbs–Stardust–PATJ complex and the Scribble–Lgl–Dlg complex (Martin-Belmonte and Mostov, 2008; Martín-Belmonte et al., 2008). However, the role of these protein complexes in maintenance of stratified epithelia polarity is not clear (Muroyama and Lechler, 2012; Niessen et al., 2012) and recent work has shown that the engulfment and cell motility protein ELMO2 positively regulates front-to-rear keratinocyte polarity by forming complexes with ILK in the presence of EGF (Ho et al., 2009; Ho and Dagnino, 2012a; Ho and Dagnino, 2012b). The data presented here identify for the first time a link between ELMO2 and type VII collagen, demonstrating that, like ELMO2, type VII collagen can regulate front-to-rear polarity (Fig. 8A). We go on to show that, like type VII collagen, ELMO2 negatively regulates SLCO1B3 (Fig. 7 and supplementary material Fig. S2) and this effect is independent of the regulation of ELMO2 by type VII collagen (supplementary material Fig. S3). We also show that Ln332 regulates SLCO1B3 independent of ELMO2 (Fig. 7E,F). Together, these data suggest that SLCO1B3 expression is not modulated by a common mechanism underlying an interaction between type VII collagen and ELMO2, but instead suggests that SLCO1B3 expression is modulated by cellular polarity.
Type VII collagen and Ln332 are secreted proteins important for epidermal keratinocyte attachment (Ogawa et al., 2007). Type VII collagen binds Ln332, a key component of hemidesmosomes and focal adhesions (Chen et al., 1999). Numerous components of these adhesion complexes have been implicated in regulating epithelial polarity (Cox et al., 2001; Martin-Belmonte and Perez-Moreno, 2012) and indeed, ELMO2 is shown to regulate front-to-rear polarity in the presence of Ln332 (Ho et al., 2009; Ho and Dagnino, 2012a). Similarly, integrin α6β4 regulates keratinocyte attachment through Ln332 and has a well-established role in directional migration (Jones et al., 1991; Dowling et al., 1996) because integrin activation of focal adhesion kinases is required for leading edge formation and directional movement (Lim et al., 2010). Integrin α6β4 also interacts with BPAG1e, promoting keratinocyte polarity through modulation of RAC1 (Hamill et al., 2009). We have shown that in the absence of type VII collagen ITGA6 and Ln332 expression is significantly reduced in 3D spheroid cultures (Fig. 8), suggesting a common mechanism, although further work is necessary to confirm this.
E-cadherin and β-catenin have described roles in numerous polarity pathways: APC (den Elzen et al., 2009), SCRIB (Navarro et al., 2005), PAR3 (Le Borgne et al., 2002) and Hippo (Kim et al., 2011); all of which are characterised by a downregulation in cancer progression (Muroyama and Lechler, 2012; Niessen et al., 2012). Our data showing that type VII collagen regulates the expression of membrane-bound E-cadherin in 3D spheroid culture is in agreement with a previous study of siRNA depletion of COL7A1 cSCC showing reduced E-cadherin in 3D organotypic culture (Martins et al., 2009). Further work will be necessary to determine the precise role that individual complexes play in type-VII-collagen-mediated polarity and organisation. Toward this end, we show here a reduction of PAR3 in RDEB cSCC spheroid culture and it is interesting to note that altered PAR3 distribution has been observed as a result of reduced E-cadherin expression (Le Borgne et al., 2002) and that deletion of PAR3 in murine epidermis does not alter normal skin development or function but does favour keratoacanthoma formation. Consequently, wild-type PAR3 mice show increased SCC formation on a background of RAS mutations, suggesting a dual role of PAR3 in skin carcinogenesis (Iden et al., 2012).
Reorganisation of polarity can have a profound effect on proteins located at the cell surface. OATP1B3 is a transmembrane transporter that localises in the basolateral domain of the plasma membrane in normal liver and the data presented here strongly support a change in polarity as an explanation for SLCO1B3 expression on the background of altered tumour cell behaviour. In addition to the identification that type VII collagen regulates OATP1B3 expression, we confirm loss of plasma-membrane-bound OATP1B3 expression in 2D cultured cells, a phenomenon also documented in primary hepatocytes (Ulvestad et al., 2011). Two recent studies have identified an N-terminal-truncated isoform of OATP1B3, OATP1B3 V1 (lacking the first 28 amino acids), as being the predominant OATP1B3 expressed in colon, lung and pancreatic cancer (Nagai et al., 2012; Thakkar et al., 2013). Thakkar and colleagues demonstrate that OATP1B3 V1 localises predominantly to the cytoplasm in culture (Thakkar et al., 2013). Our data show that membrane-localised OATP1B3 is absent in cultured SCCT8 cells, but readily detected when these cells are grown in vivo (Fig. 2C). Therefore, a more detailed analysis of OATP1B3 isoforms expressed in cSCC under these varied conditions is warranted. Because it has been suggested that OATP substrates and inhibitors can be used either to enable uptake of xenobiotics for the specific targeting of cancer cells or by inhibiting OATP-mediated transport of hormones to prevent resistance to existing therapies (Maeda et al., 2010; Svoboda et al., 2011; Obaidat et al., 2012), it will be of interest to understand the relationship between transport and isoform expression.
In summary, we show that tumour expression of SLCO1B3 in cSCC can be regulated by type VII collagen and ELMO2 and that expression of type VII collagen in RDEB SCC keratinocytes increases front-to-rear polarity and structural organisation in spheroid culture coinciding with dramatic reorganisation of the localisation of β-catenin and E-cadherin proteins.
MATERIALS AND METHODS
All human samples were collected after informed, written consent and in accordance with Helsinki guidelines. All animals were used in accordance with UK Home Office regulations.
Keratinocyte culture and xenograft tumour material
Sybr®Green qPCR validation
cDNA was prepared from RNA isolated using RNA-Bee (AMS Biotechnology, Abingdon-on-Thames, UK) and the QuantiTect reverse transcription kit (Qiagen, Manchester, UK). The 2× Sybr®Green master mix (Qiagen, Manchester, UK) along with the SLCO1B3 forward (5′-GTCCAGTCATTGGCTTTGCA-3′) and reverse (5′-CAACCCAACGAGAGTCCTTAGG-3′) primers, ELMO2 forward (5′-GGGACATGGTTTCAATCACC-3′) and reverse (5′-TGGAGGTGTGAGATGAGCTG-3′) and previously published COL7A1 primers (Wang et al., 2006) were used to quantify mRNA levels. GAPDH primers (Forward 5′-TTCCGGGAAACTGTGGCGTGA-3′ and reverse 5′-ACGGAAGGCCATGCCAG-3′) and previously published ACTB primers (Ehrenfeld et al., 2011) were used to normalise expression data. Raw data were analysed with the Rotor-Gene Q qPCR software (Qiagen, Manchester, UK) using the ΔΔCT method.
The open reading frame of SLCO1B3 and COL7A1 were cloned from an IMAGE cDNA clone (Open Biosystems, Thermo Scientific, Huntsville, AL) or normal keratinocyte cDNA, respectively, and inserted into the pBabe-puro vector using standard molecular biology techniques. RDEB cSCC keratinocytes were retrovirally transduced using the Phoenix retroviral packaging system (Kinsella and Nolan, 1996). All transduced cells were selected using 2 µg/ml puromycin. SCCRDEB3 cells were then clonally selected to generate a homogenous population expressing a high level of type VII collagen. SCCRDEB4 were also transduced as described but without clonal selection.
Immunoblotting and Immunofluorescence
Protein lysates from 2 days post confluent cells were extracted using the radioimmunoprecipitation assay (RIPA) buffer and quantified using the bicinchoninic acid (BCA) assay (ThermoFisher Scientific, Waltham, MA). 4–15% Mini-PROTEAN TGX gels (Bio-Rad, Hertfordshire, UK) were used for electrophoresis.
Formalin-fixed paraffin-embedded (FFPE), fresh frozen tissue sections and cSCC primary keratinocytes were used. Briefly, FFPE sections were rehydrated using 100%, 95% and 70% ethanol and then treated with 1% citric acid buffer (pH 6) at 100°C. FFPE, fresh frozen sections or keratinocytes cultured on coverslips were stained with the primary antibody for 1 hour followed by a 40 minute treatment with the secondary antibody and counterstained with DAPI (Invitrogen, Paisley, UK). Slides were imaged using the Zeiss Axioskop 2 fluorescent microscope (Carl Zeiss Microscopy) and analysed using the Axiovision software or the Leica TCS SP5 confocal microscope and the Leica Application Suite Advanced fluorescence Lite software (Leica Microsystems, Wetzlar, Germany).
The following antibodies were used for immunofluorescence and western blot analysis. Primary antibodies used: OATP1B3, H-52 (0.4 µg/ml; Santa Cruz Biotechnology, Dallas, TX); type VII collagen, 234192 (Calbiochem, Darmstadt, Germany); ELMO2, either the E-16 clone (0.8 µg/ml, Santa Cruz) or HPA018811 (0.4 µg/ml; Sigma-Aldrich); integrin alpha 6, 3750 (Cell Signalling, Danvers, MA); E-cadherin: (2.5 µg/ml; 610101, BD Transduction Laboratories™, San Jose, CA); Laminin 332, γ chain MAB19562 (1 µg/ml; Calbiochem); β-catenin, SC-7199 (4 µg/ml; Santa Cruz); PAR3, 07-330 (0.4 µg/ml, Calbiochem); actin, SC-58625 (0.2 µg/ml; Santa Cruz); plakogloblin, 61005 (2.5 µg/ml; Progen Biotechnik, Heidelburg, Germany). Secondary antibodies were: Alexa Fluor 594® rabbit (2.5 µg/ml; Invitrogen) or Alexa Fluor 488® mouse (2.5 µg/ml, Invitrogen)
Luciferase gene reporter assay
A luciferase gene reporter construct was created by inserting the SLCO1B3 promoter (1 kb upstream of the 5′UTR region of the gene: 11375105–11376214 of accession number: NW_001838052.1) (Jung et al., 2002) into the pGL3 basic vector using standard molecular biology techniques. SCCRDEB3 cells transduced with COL7A1 ORF or empty vector control were transfected with the SLCO1B3 promoter construct using Lipofectamine 2000 (Invitrogen) according to the manufacturer's recommendations. Cells were lysed and harvested after 18 hours and luciferase activity was recorded using the Dual-Luciferase® reporter assay system (Promega, Madison, WI) and a Modulus II plate reader (Promega). Data were normalised for transfection efficiency using Renilla and empty vector control cells were used as a calibrator.
siRNA knockdown of COL7A1, LAMC2 and ELMO2
Three siRNA constructs against COL7A1 (SASI_Hs01_00155170/71/73, Sigma-Aldrich), LAMC2 (SASI_Hs01_00136952/53/54, Sigma-Aldrich), ELMO2 (SASI_Hs01_00078453/54/55, Sigma-Aldrich) and a custom designed non-targeting control (sense: GCUGCUAGACGUGUUAUGA[dT][dT], antisense: UCAUAACACGUCUAGCAGC[dT][dT], Sigma-Aldrich) were transfected using Lipofectamine 2000 (Invitrogen). RNA was extracted 24, 48 and 72 hours post transfection and analysed for COL7A1, SLCO1B3, LAMC2 and ELMO2 expression using Sybr®Green qPCR.
An ELMO2 cDNA construct (MHS1010-57517, Thermo Scientific) and the empty vector control was transfected into cSCC keratinocytes using Lipofectamine 2000 (Invitrogen). RNA and protein was harvested 17 hours and 24 hours post transfection and assessed for ELMO2 overexpression using Sybr®Green qPCR.
SCCRDEB3-C7 and SCCRDEB3-EV cells were cultured in a six-well plate until 2 days post confluent. Cells were washed with cold PBS and lysed with 1 ml 0.5% sodium deoxycholate for 10 minutes on ice. Lysed cells were removed and wells were washed with dH2O and air dried. SCCRDEB2 and SCCRDEB3 parental cells were seeded on either collagen VII containing matrix or empty vector control matrix for 3 days before RNA and protein samples were harvested.
Cell plating lamellipodia scoring
500,000 SCCRDEB3-C7 and SCCRDEB-EV cells were seeded in a 10 cm dish. Cells were imaged 5 hours post seeding. Twenty frames were scored blind for each cell type and data was analysed from three independent experiments. Clear cellular protrusions were counted for lamellipodia scoring and the difference in cell diameter was observed in order to accurately score for cell spreading. A Leica TCS SP5 confocal microscope was used to image cells after staining with β-actin and analysed using the Leica Application Suite Advanced fluorescence Lite software (Leica Microsystems, Wetzlar, Germany).
SCCRDEB3 type VII collagen overexpressing (SCCRDEB3-C7) and the empty vector (SCCRDEB3-EV) cells were cultured for 72 hours in a hanging-drop non-adherent culture. Briefly, 6000 cells were seeded on the lid of a 10 cm culture dish and allowed to form spheroids. Each spheroid was washed with PBS and harvested using a syringe and placed in 4% paraformaldehyde overnight. Spheroids were washed with PBS next day and placed at 4°C and embedded in paraffin. Alternatively, spheroids were washed with PBS and directly frozen in OCT. Spheroids were serially sectioned for immunofluorescence.
Fibroblasts were transduced with COL7A1 or empty vector controls. 3000 fibroblasts were seeded in 20 µL medium on a sterile 10 cm cell culture dish lid and incubated for four days at 37°C. On day four, 10 µl of culture medium was removed from each droplet. 6000 keratinocytes were seeded in 10 µl of medium onto each droplet of previously formed fibroblasts spheroids and cultured for 3 days. Spheroids were washed with PBS and embedded in OCT for serial sectioning and staining. In parallel, and to visualise the separate populations, cells were labelled with PKH67 green fluorescent cell linker kit (fibroblasts) or PKH26 red fluorescent cell linker kit (keratinocytes) according to the manufacturer's protocol (Sigma-Aldrich). Labelled spheroids were visualised using a Carl Zeiss Axiovert25 CFL microscope.
Student's two tailed t-tests were used to determine statistical significance of luciferase gene reporter assays and COL7A1 siRNA knockdown data.
We would like to thank Karl Gledhill for technical assistance.
A.P.S. initiated and supervised the work. A.P.S., J.H.S.D., C.L.C., C.P., S.A.W. and Y.Z.L. conducted experiments. All authors participated in data interpretation and manuscript preparation.
This work was funded by the European Research Council (ERC) [grant number 250170]; DEBRA UK and DEBRA International – funded by DEBRA Austria. I.M.L. is funded by a strategic grant from the Wellcome Trust. Deposited in PMC for release after 6 months.
The authors declare no competing interests.