Stromal interaction molecule 1 (STIM1) is an endoplasmic reticulum (ER) Ca2+ sensor that triggers the store-operated Ca2+ entry (SOCE). The clinical relevance of STIM1 has been highlighted in breast and cervical cancer, but the molecular mechanism by which STIM1 promotes cancer progression remains unclear. This study explores the regulatory mechanisms by which STIM1-dependent Ca2+ signaling controls cancer cell migration. Three different SOCE inhibitors, SKF96365, 2-APB and YM-58483, significantly inhibited cervical cancer cell migration to a similar extent to that of STIM1 silencing. In contrast, STIM1 overexpression significantly enhanced cervical cancer cell migration. Live cell confocal images and three-dimensional tomograms showed that STIM1 formed aggregates and translocated towards the plasma membranes of migratory cells, and this was accompanied by increasing cytosolic Ca2+ spikes. STIM1 silencing also inhibited the recruitment and association of active focal adhesion kinase (pTyr397-FAK) and talin at focal adhesions, indicating the blockade of force transduction from integrin signaling. Epidermal growth factor-induced phosphorylation of myosin II regulatory light chains was abolished by STIM1 knockdown and SOCE inhibition. Dual immunostaining of activated myosin II (pSer19-MLC) and actin revealed that actomyosin formation depended on STIM1-mediated Ca2+ entry. Most importantly, STIM1 expression levels as well as SOCE activity controlled the generation of cell contractile force, as measured by the microfabricated post-array-detector system. These results highlight the unique role of STIM1-dependent Ca2+ signaling in controlling cell migration by the regulation of actomyosin reorganization in conjunction with enhanced contractile forces.
Cell migration is an essential process during embryonic development, wound healing, angiogenesis and immunological chemotaxis. The cycling of multi-step processes is involved in cell migration, such as front edge extension, new adhesion formation, cytoskeletal contraction, and rear end detachment (Ridley, 2011). Cell migration is also an early requirement for cancer metastasis when cancer cells acquire invasive behavior and disseminate through the nearby blood and lymph vessels (Mierke et al., 2008; Yang et al., 2009; Yilmaz and Christofori, 2009). Migratory cells exert contractile force to supply the rear-end retraction and forward protrusion (Ananthakrishnan and Ehrlicher, 2007). While these contractile forces are important for the regulation of focal adhesion turnover and cytoskeletal organization in cell migration, the mechanisms that control force generation during cell migration are not well understood.
Modulation of cytosolic Ca2+ levels provides versatile and dynamic signaling involved in various cellular processes, such as proliferation, migration and gene regulation (Berridge et al., 2003). Store-operated Ca2+ entry (SOCE) is a major Ca2+ entry pathway in nonexcitable cells (Parekh, 2010; Putney, 2005). SOCE, by definition, is activated by Ca2+ release from the internal store. The activation of SOCE includes several steps. (1) Stimulation of G proteins or protein tyrosine kinases activates phospholipase C (PLC), which hydrolyzes phosphatidylinositol bisphosphate to release the second messenger inositol 1,4,5-trisphosphate. (2) Binding of inositol 1,4,5-trisphosphate to its receptor in the endoplasmic reticulum (ER) membrane causes rapid and transient Ca2+ release from the ER lumen. (3) The decrease of ER luminal Ca2+ activates store-operated Ca2+ (SOC) channels in the plasma membrane, leading to a sustained influx of extracellular Ca2+ across the plasma membrane (reviewed in Parekh, 2010; Várnai et al., 2009). Two genes, STIM1 (stromal-interaction molecule 1) and Orai1, are responsible for SOCE activation (Parekh, 2010). The STIM1 molecule functions as an ER Ca2+ sensor that detects store depletion. Once ER Ca2+ is depleted, STIM1 proteins form many aggregates and translocate close to the plasma membrane. Orai1, an essential pore-forming component of the SOC channel, translocates to the same STIM1-containing structures during store depletion and opens to mediate Ca2+ entry.
The physiological importance of STIM1 has mostly been studied in immune systems. STIM1 is required for the development and function of regulatory T cells (Oh-Hora et al., 2008; Qu et al., 2011) and STIM1 deficiency causes several autoimmune diseases and myopathy in human subjects and mouse models (Feske, 2009). The emerging importance of STIM1 in tumor biology has been highlighted in breast and cervical cancer (Chen et al., 2011; Yang et al., 2009). Inhibiting STIM1-mediated Ca2+ influx impairs the focal adhesion turnover of breast cancer cells, which can be rescued by the small GTPases Ras and Rac (Yang et al., 2009). In addition, reduction of Orai1 or STIM1 by RNA interference in highly metastatic human breast cancer cells, or treatment with a pharmacological inhibitor of SOCE, decreases tumor metastasis in animal models. Our previous study further demonstrated that STIM1 is a key regulator for the epidermal growth factor (EGF)-induced Ca2+ influx that is necessary for the activation of Ca2+-dependent protease calpain and tyrosine kinase Pyk2 (Chen et al., 2011). More importantly, our findings from analyzing surgical specimens suggested that the pathological significance of tumor STIM1 overexpression is that it increases the locomotion of cancer cells.
The efficiency of cell migration is regulated by the dynamic interactions between the cytoskeleton, non-muscle myosin II and focal adhesions (Kadir et al., 2011). The association between actin filaments and myosin II forms contractile actomyosin fibers that regulate various biological processes, including cytokinesis, adhesion and migration (Sanz-Moreno et al., 2011). Although our and other studies have demonstrated that STIM1-dependent signaling regulates focal adhesion turnover and cell migration (Chen et al., 2011; Yang et al., 2009), an important remaining question is whether STIM1-dependent signaling integrates the dynamic interactions between actomyosin and focal adhesions to mediate efficient cell migration. In the current study we demonstrated that STIM1 expression and SOCE activity are necessary for cell migration through the regulation of actomyosin reorganization in conjunction with enhanced contractile forces.
Cancer cell migration depends on STIM1 expression and SOCE activity
We established several stable pools of SiHa cervical cancer cells with different levels of STIM1 expression to study its role in cell migration (Fig. 1A). Transfection with STIM1-specific shRNAs optimally reduced STIM1 protein levels. In the presence of STIM1-specific shRNAs, the migration of SiHa cells was reduced by 40–50% (Fig. 1B). In contrast, STIM1 overexpression significantly enhanced cervical cancer cell migration. We also studied whether the blockade of SOCE activity alters cervical cancer cell migration. SKF96365 and 2-APB are potent inhibitors of SOCE in cervical cancer cells (Chen et al., 2011). YM-58483 also inhibited SOCE of cervical cancer cells in a dose-dependent manner (supplementary material Fig. S1). SKF96365 significantly inhibited cervical cancer cell migration to a similar extent by STIM1 silencing (Fig. 1B). Moreover, both 2-APB and YM-58483 dose-dependently inhibited cervical cancer cell migration. These results indicate that STIM1 expression levels, as well as SOCE activity, are associated with cervical cancer cell migration.
The polarized distribution of intracellular Ca2+ is known to be involved in the regulation of cell migration (Wei et al., 2009). We previously showed that STIM1-dependent Ca2+ signaling played an important role in EGF-stimulated cancer cell migration (Chen et al., 2011). EGF stimulates the aggregation and translocation of STIM1 towards to the Orai1-containing regions of the plasma membrane to mediate SOCE (Chen et al., 2011). It is thus of interest to monitor the spatial and temporal distribution of cytosolic Ca2+ spikes and STIM1 in migratory cells. We performed time-lapse confocal image recordings of cervical cancer cells upon EGF stimulation (supplementary material Movie 1). As shown in Fig. 2A,B, STIM1 was scattered in the cytosol prior to EGF stimulation. EGF induced STIM1 aggregation into many clusters that localized to regions near or at the plasma membrane, especially at both polar ends of migratory cells. The translocation of STIM1 clusters became obvious 5–10 minutes after EGF stimulation. The three-dimensional (3D) tomograms also indicated that EGF stimulated the formation of STIM1 aggregates that translocated towards the plasma membrane (Fig. 2C; supplementary material Movies 3, 4). Moreover, the STIM1 aggregation was accompanied by increased cytosolic Ca2+ spikes, as visualized by the Ca2+-sensitive fluorescent dye Fluo-4 (Fig. 3A,B; supplementary material Movie 4).
STIM1-dependent Ca2+ signaling regulates actomyosin formation
Migratory cells generate intracellular forces that act on the surrounding extracellular matrix or neighboring cells (Milner and Campbell, 2002). This contractile activity is crucial for a number of signal transductions that regulate cell migration. The contractile forces of migratory cells are regulated by dynamic focal adhesions and also controlled by actomyosin contractility. The interaction between focal adhesion kinase (FAK) and talin is required for focal adhesion turnover and cell motility (Lawson et al., 2012). FAK is a cytoplasmic tyrosine kinase that phosphorylates targets such as paxillin to regulate adhesion dynamics. Talin is a large cytoskeletal protein that binds integrin cytoplasmic tails and FAK, and plays a key role in integrin-mediated force transduction and cell migration. We studied the effect of STIM1 expression on the force transduction of focal adhesions by double-staining of pTyr397-FAK and talin, which represents the functional layers of integrin signaling and force transduction (Kanchanawong et al., 2010). As shown in Fig. 4, EGF induced the recruitment of active FAK (pTyr397-FAK) to focal adhesions, where pTyr397-FAK was associated with variably sized, diagonally oriented arrays of talin. In contrast, STIM1 knockdown abolished EGF-induced recruitment and association of pTyr397-FAK and talin at focal adhesions, indicating that STIM1 expression is necessary for the force transduction of the focal adhesion network.
Non-muscle cell contractility is controlled by non-muscle myosin II, through the phosphorylation of its regulatory light chains (MLC2) in a Ca2+/calmodulin-dependent manner (Vicente-Manzanares et al., 2009). We studied whether STIM1-dependent Ca2+ signaling and SOCE activity are involved in the regulation of MLC2 activation. As shown in Fig. 5A, EGF significantly increased the phosphorylation levels of MLC2 at Ser19 (pSer19-MLC) that had been almost abolished by SKF96365, a SOCE inhibitor in cervical cancer cells (Chen et al., 2011). STIM1 knockdown by siRNA also significantly attenuated EGF-induced MLC2 phosphorylation at Ser19 (Fig. 5B). These results indicate that STIM1-dependent Ca2+ signaling have a great impact on MLC2 activation.
We further studied the association between EGF stimulation, STIM1 abundance and actomyosin contractility. Actomyosin is a complex of actin filaments and non-muscle myosin II. To study the expression of actomyosin, SiHa cells were double-immunostained with activated myosin II (pSer19-MLC) and actin. As shown in Fig. 6A, the quiescent cells had well-organized cortical actin cytoskeletons at the cell periphery, and the intensity of pSer19-MLC fluorescence was scanty. EGF stimulation induced the reorganization of actin stress fibers at cell protrusions, where pSer19-MLC aligned along the actin filaments, indicating the increased expression of actomyosin (Fig. 6A). STIM1 knockdown by siRNA almost abolished EGF-induced actomyosin formation compared with control siRNA treatment (Fig. 6B). In contrast, STIM1 overexpression significantly enhanced actomyosin expression in response to EGF stimulation, compared to that of mock transfection (Fig. 6C,D). As summarized in Fig. 6E, EGF-induced reorganization of actomyosin in cervical cancer cells depends on STIM1 abundance. The role of Ca2+ entry in regulating actomyosin formation was also studied. Blocking Ca2+ influx by removal of external Ca2+ almost abolished EGF-stimulated actomyosin formation (Fig. 7A,B). In addition, results from three different SOCE inhibitors demonstrated that SOCE activity is involved in the regulation of actomyosin reorganization (Fig. 7C,D). Taken together, these results indicated that STIM1-mediated SOCE is an important pathway controlling actomyosin reorganization.
SOCE and STIM1 are involved in the regulation of contractile force
Cell migration involves dynamic adhesive processes and cytoskeleton remodeling, leading to contraction between cells and the surrounding extracellular matrix. The current study and our previous results (Chen et al., 2011) show that STIM1 was crucially involved in EGF-induced focal adhesion turnover and actomyosin reorganization. To reveal the important role of STIM1 in the regulation of cell contraction, the microfabricated post-array-detector (mPAD) system was used to measure cell contractile force at a single adhesion site (Fu et al., 2010; Tan et al., 2003). The micropillars of mPAD were 3 µm in diameter, 8 µm tall, and were configured in a regular array with 9 µm center-to-center spacing (supplementary material Fig. S2). We stained the mPAD with 1,1′-dioctadecyl-3,3,3′3′-tetramethylindocarbocyanine perchlorate (DiI), a hydrophobic and lipophilic cyanine dye, for visualization with confocal microscopy. The top of the pillar was coated with fibronectin to assist cell adhesion. SiHa cells were cultured on mPADs and the whole system was scanned using confocal microscopy (Fig. 8A; supplementary material Movie 5). Cells generated contractile force pulling on the adherent micropillars (Fig. 8B). The contractile force (F) on the micropillars was calculated by the displacement (ΔX) and spring constant of the micropillars (Fig. 8C). The displacement of micropillars was measured as the deviation of the top of the pillar with reference to the bottom (Fig. 8D, top and bottom of the micropillar are colored grey and red, respectively). This method thus provides a quantitative and temporal definition of the nanonewton (nN)-ranged traction force between the cells and the extracellular matrix at the micropillars (Fu et al., 2010; Tan et al., 2003).
SiHa cells were cultured on the mPAD system under various experimental conditions to evaluate the effect of STIM1 expression and SOCE activity on cell contractility. Fig. 8D showed the real-time measurements of traction force at single micropillar generated by STIM1-overexpressing SiHa cells in response to EGF stimulation. The cell contractile force is defined as the sum of the traction force applied at each micropillar (Fig. 8E). Compared with mock-transfected cells, STIM1 overexpression significantly enhanced the amplitude of the contractile force (Fig. 8E). More importantly, both the SOCE inhibitor SKF96365 and the intracellular Ca2+ chelator BAPTA-AM abolished EGF-induced contractile force generation (Fig. 8E). These results indicate that STIM1-dependent Ca2+ signaling and SOCE activation induce actomyosin reorganization in conjunction with enhanced contractile forces.
This study highlights the important role of STIM1-dependent Ca2+ signaling in controlling cell migration. STIM1 expression levels as well as SOCE activation are functionally linked with cell migration through the regulation of actomyosin reorganization in conjunction with enhanced contractile forces. This conclusion is supported by the following evidence. (1) STIM1 overexpression enhanced cervical cancer cell migration, whereas RNA interference targeting STIM1 suppressed it. Blocking SOCE by the use of inhibitors decreased cervical cancer cell migration to a similar extent as that seen with STIM1 knockdown, suggesting that STIM1-dependent SOCE plays an important role in cervical cancer cell migration. (2) STIM1 knockdown inhibited the recruitment and association of pTyr397-FAK and talin at focal adhesions, indicating the blockade of force transduction from integrin signaling. (3) MLC2 phosphorylation was closely associated with STIM1 expression as well as SOCE activity. Consistent with this, actomyosin formation in migratory cells depended on STIM1-mediated Ca2+ entry. (4) The direct measurement of cell contractility confirmed that the generation of cell contractile force required STIM1-dependent SOCE.
In this study, we demonstrated that STIM1-mediated SOCE plays an important role in regulating cervical cancer cell migration. Interestingly, both STIM1 knockdown with two different shRNA duplexes and SOCE blockade with three different potent inhibitors significantly reduced cervical cancer cell migration by 50% (Fig. 1B), indicating that other Ca2+ entry pathways contributed to cervical cancer cell migration. Several plasmalemmal Ca2+ channels have been suggested as important in regulating cancer cell migration (reviewed in Prevarskaya et al., 2011; Monteith et al., 2012). For example, the Ca2+-permeable transient receptor potential (TRP) channel family has been implicated in migration of various cancer cell types. A pro-migratory role of TRPM7 was demonstrated in breast cancer cells, in which TRPM7 silencing interfered with focal adhesion dynamics and significantly decreased cellular migratory potential (Middelbeek et al., 2012). In prostate cancer cells, it has been shown that the expression of TRPV2 was correlated with cell migration through the induction of the invasive enzymes matrix metalloproteinase (MMP)-2, MMP-9 and cathepsin B (Monet et al., 2010). It thus remains possible that SOCE activity is an important pathway, but not the only one, controlling cervical cancer cell migration.
Our results showed the unique role of STIM1 in controlling the mechanotransduction of migratory cells. Migratory cells can generate intracellular forces that act on the surrounding extracellular matrix or neighboring cells, and this contractile activity is important for several signal transductions essential for cell migration. Non-muscle myosin II is the major motor protein driving cellular contractility. The bipolar filaments of myosin II pull together antiparallel oriented actin filaments to generate cortical tension. The phosphorylation of regulatory light chains by Ca2+-dependent MLC kinase (MLCK) is the major regulator for the myosin-II-based contractile responses. Myosin-II-based actomyosin contraction is transmitted to focal adhesions to establish contractile force that relocates the cell body and contributes to cell movement. The importance of STIM1-dependent Ca2+ signaling in focal adhesion turnover during cancer cell migration has been demonstrated in previous studies (Chen et al., 2011; Yang et al., 2009). By the direct measurement of cell traction forces, our results showed for the first time that STIM1-dependent Ca2+ signaling regulated the traction force generation at focal adhesions. The influence of STIM1 abundance on contractile force generation was consistent with that on actomyosin reorganization, as visualized by immunostaining of activated myosin II molecules and actin stress fibers.
Taken together with our previous study (Chen et al., 2011), we propose the following mechanisms by which STIM1-dependent Ca2+ signaling regulates cell force transmission during migration. One involves integrin signaling, which regulates the recruitment of Pyk2, FAK and talin to focal adhesions, and the other controls the formation of actomyosin by regulating phosphorylation of the myosin II regulatory light chain. In conclusion, the interaction between STIM1 and the plasma membrane SOC channel Orai1 activates the Ca2+ influx that induces actomyosin reorganization in conjunction with enhanced contractile forces (summarized in supplementary material Fig. S3). This study suggests that blocking STIM1-mediated Ca2+ signaling is a potential approach to inhibit cancer cell migration.
Materials and Methods
Cell culture and transfection
Cultures of SiHa human cervical cancer cells were prepared as previously described (Chen et al., 2011; Chiu et al., 2008). Ca2+-free Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Invitrogen. Stable pools of cervical cancer cells overexpressing human STIM1 were used in this study. The full-length cDNA of EGFP-STIM1 and mOrange-STIM1 were kindly provided by Liangyi Chen of the Chinese Academy of Science, China (Xu et al., 2006). EGFP- or mOrange-STIM1 cDNA plasmids were transfected into SiHa cells using Lipofectamine 2000 (Invitrogen) and cells with STIM1 overexpression were selected by G418 (Sigma-Aldrich). A FACSAria cell sorter (BD Biosciences) was used to isolate the stable pools of cells overexpressing human STIM1.
The details of RNAi targeting and preparation were described previously (Chen et al., 2011). For small interfering RNA (siRNA)-mediated knockdown of STIM1, cells were transfected with 100 nM of either the targeting or control siRNA (Santa Cruz Biotechnology) using Lipofectamine 2000 (Invitrogen) for 48 hours. The functional assays were subsequently carried out after validation of STIM1 knockdown without affecting the cell viability. Several independent duplexes of siRNAs (synthesized by Invitrogen) and the siRNA pools of three duplexes (Santa Cruz Biotechnology) were used to target each gene. The sequences of siRNA targeting human STIM1 are as follows: (i) sense, 5′-UUGAGAGGAAACUUCUUCCGGCCUGtt-3′; antisense, 5′-CAGGCCGGAAGAAGUUUCCUCUCAAtt-3′; (ii) sense, 5′-UAACGGUUCUGGAUAUAGGCAAACCtt-3′; antisense, 5′-GGUUUGCCUAUAUCCAGAACCGUUAtt-3′; (iii) the STIM1 siRNA pool of three duplexes: sense, 5′-CUGGUGGUGUCUAUCGUUAtt-3′; antisense, 5′-UAACGAUAGACACCACCAGtt-3′; sense, 5′-CCACUUCAGUGCAUGUCUUtt-3′; antisense, 5′-AAGACAUGCACUGAAGUGGtt-3′; sense, 5′-CAGGAGGAUUUGUCUCUAAtt-3′; antisense, 5′-UUAGAGACAAAUCCUCCUGtt-3′. To establish stable pools of SiHa cells with STIM1 knockdown, two different pools of short hairpin RNAs (shRNAs) targeting human STIM1 (from Santa Cruz Biotechnology and OriGene) or control shRNA (Santa Cruz Biotechnology) were introduced into SiHa cells by electroporation (MicroPorator-mini MP-100, Digital Bio Technology), and selected using 350 ng/ml puromycin (Sigma-Aldrich). The sequences of shRNA pools targeting human STIM1 are as follows: (i) 5′-CTGGTGGTGTCTATCGTTATTCAAGAGATAACGATAGACACCACCAGTTT-3′; 5′-CAGGAGGATTTGTCTCTAATTCAAGAGATTAGAGACAAATCCTCTGTTTTT-3′ (Santa Cruz Biotechnology). (ii) 5′-GATGATGCCAATGGTGATGTGGGATGTGGA-3′; 5′-ACAGTGAAACATAGCACCTTCCATGGTGA-3′; 5′-CTGCTGGTTTGCCTATATCCAGAACCGTT-3′; 5′-CTGAGCAGAGTCTGCATGACCTTCAGGAA-3′ (OriGene).
Primary antibodies and reagents
The antibody against STIM1 (clone 44) was from BD Biosciences. The antibody against β-actin (clone AC-15) was from Sigma-Aldrich. The antibody against phospho-Tyr397-FAK (catalog number 44-624G) was from Invitrogen. Antibodies against myosin-light cahin-2 (MLC-2; catalog number 3672) and phospho-Ser19-MLC (pMLC; catalog number 3671) were from Cell Signaling. The antibody against talin (clone TD77) was from Millipore. EGF and YM-58483 were from Sigma-Aldrich. SKF96365 and 2-APB were from Cayman Chemical. 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetra(acetoxymethyl) ester (BAPTA-AM) was from Merck-Millipore. Fluo-4/AM was from Invitrogen.
For immunoblotting, cells were harvested with ice-cold modified radioimmune precipitation assay (RIPA) buffer containing a protease inhibitor cocktail (Roche Diagnostics), 100 mM KCl, 80 mM NaF, 10 mM EGTA, 50 mM h-glycerophosphate, 10 mM p-nitrophenyl phosphate, 1 mM vanadate, 0.5% sodium deoxycholate, and 1% NP40. Protein concentrations were determined with the use of a Bio-Rad protein assay. Equal amounts of protein lysates were separated by SDS-PAGE, and then transferred to nitrocellulose blotting membranes (Pall). Immunoblots were blocked, incubated with the primary antibody, washed, and incubated with the corresponding horseradish-peroxidase-conjugated secondary antibody (Jackson ImmunoResearch), and visualized by western blotting luminol reagent (Santa Cruz Biotechnology). Bands in the immunoblots were quantified using Vision WorksLS software (UVP).
For cell migration assay, cells were allowed to migrate across a Transwell (with 8 µm pore membranes; Corning Incorporated) towards the medium containing 10 µg/ml fibronectin for 12 hours at 37°C. Cells that migrated through the membrane were then fixed with methanol, stained with GIEMSA stain, and counted immediately.
Immunofluorescence, confocal microscopy and image analyses
For immunofluorescent staining, cells were seeded on glass coverslips. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 for 15 min, and blocked with commercial blocking serum (Invitrogen). The cells were incubated with primary antibody at 4°C overnight, washed, and then incubated with Alexa-Fluor-conjugated secondary antibodies (Invitrogen) for 1 hour at room temperature. To detect nuclei and actin filaments, cells were stained with Hoechst 33258 and phalloidin conjugated with tetramethylrhodamine B isothiocyanate (TRITC; Sigma-Aldrich) for 1 hour at room temperature. The cells were then washed and mounted, and the fluorophores were excited by a laser at 405, 488, or 543 nm and examined with a scanning confocal microscope (FV-1000, Olympus). For the live-cell imaging, cells overexpressing EGFP–STIM1 were directly activated by a laser at 488 nm for the detection of EGFP. The fluorescent Ca2+ indicator Fluo4/AM (Invitrogen) was used to estimate cytosolic Ca2+ levels. The colocalization of different molecules in confocal images was assessed using pixel-by-pixel analysis and FV-1000 software. The 3D images of mPADs and the 3D tomography of STIM1 were reconstructed from serial confocal Z-section scanning images using Avizo 3D imaging and analysis software (Version 6.0, Mercury computer systems).
Preparation of the mPAD system and measurement of contractile forces
The fabrication of the microfabricated post-array-detector (mPAD) system and contractile force measurement were performed as previously described (Fu et al., 2010; Tan et al., 2003), following the procedure illustrated in the supplementary material Fig. S3. The contractile force on each adhesion site was calculated using Hooke’s Law and the displacement and spring constant of the micropillars (Fig. 8C). The displacement was measured as the deviation of the top (deflected position) from the bottom (un-deflected position) of the micropillar from serial confocal Z-section scanning images.
All values are reported as means ± s.e.m. (standard error of the mean). Student’s paired or unpaired t-tests were used for statistical analyses. Differences between values were considered significant when P<0.05.
We thank Dr Liangyi Chen at the Chinese Academy of Science for STIM1 plasmids.
Y.T.C., Y.F.C., W.T.C., Y.K.W., H.C.C., and M.R.S. designed research; Y.T.C., Y.F.C., W.T.C., and Y.K.W. performed research; Y.T.C., Y.F.C., W.T.C., Y.K.W., and M.R.S. analyzed data; and Y.T.C., Y.F.C., and M.R.S. wrote the article.
This work was partly supported by National Science Council [NSC 101-2321-B-006-019 to M.R.S.], National Health Research Institutes [NHRI-EX102-10243BI to M.R.S.], Department of Health, Executive Yuan and National Cheng Kung University Hospital, Taiwan.