Vascular endothelial growth factor (VEGF) and transforming growth factor-β (TGFβ) are potent regulators of angiogenesis. How VEGF and TGFβ signaling pathways crosstalk is not well understood. Therefore, we analyzed the effects of the TGFβ type-I-receptor inhibitors (SB-431542 and LY-2157299) and VEGF on endothelial cell (EC) function and angiogenesis. We show that SB-431542 dramatically enhances VEGF-induced formation of EC sheets from fetal mouse metatarsals. Sub-optimal doses of VEGF and SB-431542 synergistically induced EC migration and sprouting of EC spheroids, whereas overexpression of a constitutively active form of TGFβ type-I receptor had opposite effects. Using quantitative PCR, we demonstrated that VEGF and SB-431542 synergistically upregulated the mRNA expression of genes involved in angiogenesis, including the integrins α5 and β3. Specific downregulation of α5-integrin expression or functional blocking of α5 integrin with a specific neutralizing antibody inhibited the cooperative effect of VEGF and SB-431542 on EC sprouting. In vivo, LY-2157299 induced angiogenesis and enhanced VEGF- and basic-fibroblast-growth-factor-induced angiogenesis in a Matrigel-plug assay, whereas adding an α5-integrin-neutralizing antibody to the Matrigel selectively inhibited this enhanced response. Thus, induction of α5-integrin expression is a key determinant by which inhibitors of TGFβ type-I receptor kinase and VEGF synergistically promote angiogenesis.
During embryogenesis, the formation of new blood vessels depends on vasculogenesis and angiogenesis. Angiogenesis refers to the formation of new blood vessels from pre-existing ones (Carmeliet and Jain, 2000), and consists of an activation phase and a resolution phase (Folkman and D'Amore, 1996). The activation phase is associated with vessel destabilization and increased permeability, degradation of the extracellular matrix (ECM), and endothelial cell (EC) proliferation and migration. During the resolution phase, ECs become quiescent and pericytes and vascular smooth muscle cells (VSMCs) are recruited to ensure stabilization and maturation of the newly formed vessels (Carmeliet and Jain, 2000).
Angiogenesis is tightly regulated by pro- and anti-angiogenic signals, and plays an important role in pathophysiological and physiological processes such as wound healing, tissue remodeling, the female reproductive cycle, autoimmune diseases and cancer (Folkman, 2007). Vascular endothelial growth factor (VEGF) is a key regulator of vasculogenesis and angiogenesis. Heterozygous mice lacking a single VEGF allele die at embryonic day (E)8.5 with severe vascular defects (Carmeliet et al., 1996; Ferrara et al., 1996). Overexpression of VEGF also results in embryonic lethality (Miquerol et al., 2000). VEGF signals through two distinct tyrosine-kinase receptors, VEGFR1 (also known as Flt-1) and VEGFR2 (also known as KDR and Flk-1) (Mustonen and Alitalo, 1995), and exerts multiple effects on ECs, including proliferation, survival (Darland et al., 2003), migration and the formation of capillary-like tubules (Gerhardt et al., 2003).
Genetic studies in mice and humans have suggested that perturbation of TGFβ signaling results in vascular abnormalities (ten Dijke and Arthur, 2007). TGFβ exerts its biological effects by binding to and activating type-I and type-II transmembrane serine/threonine-kinase receptors. Binding of TGFβ to the TGFβ type-II receptor (TβRII) leads to recruitment and phosphorylation of type-I receptor [TβRI, or activin receptor like kinase 5 (ALK5)]. Activin and Nodal, which are structurally related to TGFβ, signal via ALK4 and ALK7, respectively. Activated ALK5, ALK4 and ALK7 propagate the signal into the cells by phosphorylating the downstream effector proteins Smad2 and Smad3 (ten Dijke and Arthur, 2007). In ECs, TGFβ can also activate ALK1, an alternate type-I receptor, which mediates phosphorylation of Smad1 and Smad5 (Goumans et al., 2002; Goumans et al., 2003). The TGFβ-ALK5 pathway results in inhibition of EC proliferation and migration, whereas the TGFβ-ALK1 pathway results in the activation of proliferation and the migration of ECs. Balance between the TGFβ-ALK1 and TGFβ-ALK5 signaling pathways plays an important role in angiogenesis (Goumans et al., 2002; Goumans et al., 2003). In line with those results, the effects of TGFβ on angiogenesis are highly context-dependent, e.g. at low concentrations, TGFβ promotes angiogenesis, whereas, at high concentrations, it inhibits it (Yang and Moses, 1990). In addition to its direct effects, TGFβ can exert effects on angiogenesis by regulating the expression of angiogenic factors such as VEGF and components of the ECM such as matrix metalloproteases (MMPs) and integrins (Duivenvoorden et al., 1999; Edwards et al., 1987; Sehgal and Thompson, 1999).
Integrins are heterodimeric transmembrane proteins consisting of α- and β-subunits mediating cell-ECM interactions. Up to date, nine integrin heterodimers have been implicated in blood-vessel formation, namely α1β1, α2β1, α4β1, α5β1, αvβ1, αvβ3, αvβ5, αvβ8 and α6β4 (Avraamides et al., 2008). Despite the fact that integrins lack intrinsic enzymatic activity, upon ligand-induced integrin clustering specific intracellular signals are initiated by the activation of intracellular associated kinases and adaptor proteins in focal-adhesion complexes. Integrins regulate divergent biological events including cell adhesion, migration, proliferation, differentiation, survival and angiogenesis (Avraamides et al., 2008).
Several studies have provided evidence suggesting that the interplay of VEGF and TGFβ signaling pathways plays an important role in angiogenesis. TGFβ can induce the expression of VEGF by various cells in the tumor microenvironment, such as tumor cells, stromal fibroblasts and cells of the immune system (Sanchez-Elsner et al., 2001; Teraoka et al., 2001). SB-431542, an ALK5/4/7 kinase inhibitor (Laping et al., 2002), was shown to exert an inhibitory effect on VEGF secretion in human cancer cell lines (Halder et al., 2005; Hjelmeland et al., 2004; Matsuyama et al., 2003). Moreover, it was shown that SB-431542 stimulated the formation of FLK1-positive embryonic stem cell (ESC)-derived EC sheets induced by VEGF (Watabe et al., 2003).
However, the molecular mechanisms that regulate the cross-talk between the VEGF and TGFβ signaling pathways in angiogenesis have not been determined. To elucidate the interplay between VEGF and ALK5, we analyzed the effects of VEGF and SB-431542 alone or in combination using different angiogenesis assays. We show that VEGF and SB-431542 synergistically induce angiogenesis both in vitro and in vivo. Gene expression profiling and functional validation revealed that the upregulation of α5-integrin expression plays a crucial role by which VEGF and TGFβ-type-I-receptor-kinase inhibitor achieve their synergistic angiogenic response.
VEGF and ALK5-kinase inhibitor synergistically enhance angiogenesis in fetal mouse metatarsal assay
To investigate the interplay between VEGF and TGFβ-ALK5 signaling in angiogenesis, we made use of an ex vivo fetal mouse metatarsal assay. This model provides a quantitative ex vivo assay with the complexity of an in vivo assay to study the formation of capillary-like structures (Deckers et al., 2001; van der Pluijm et al., 1991). Metatarsals of 17-day-old mouse embryos were isolated and cultured in 24-well plates for 72 hours to allow adherence, followed by stimulation with VEGF, TGFβ3, SB-431542 or combinations thereof. Capillary-like structures were visualized by staining the cultures using an EC-specific anti-CD31 antibody (Fig. 1A). Similar to what was previously reported for VEGF and TGFβ2 (Deckers et al., 2001), quantitative image analysis showed that VEGF strongly stimulated the formation of vessel-like structures, which was inhibited by TGFβ3 (Fig. 1). Treatment of bone explants with the ALK4/5/7 inhibitor SB-431542 had no significant effects on basal outgrowth of tube-like structures. However, the combination of VEGF with SB-431542 (VEGF+SB-431542) significantly stimulated EC network formation in a synergistic manner. Interestingly, treatment of metatarsals with LY-2157299, another ALK5-kinase inhibitor that is structurally divergent from SB-431542 (Bueno et al., 2008), slightly inhibited endothelial sheet formation. However, similar to SB-431542, LY-2157299 strongly promoted VEGF-induced angiogenesis (Fig. 2A,C). Our results indicate that activation of VEGF and inhibition of TGFβ (and/or Activin and/or Nodal) signaling by the SB-431542 or LY-2157299 inhibitors synergistically stimulates angiogenesis in vitro.
ALK5 inhibitor and VEGF synergize in inducing EC sprouts in 3D spheroid culture
To study the effect of combined VEGF and ALK5 inhibitor on EC function, we performed a three-dimensional (3D)-culture collagen EC spheroid assay, which is a suitable model for the analysis of the early regulation of angiogenesis (Korff and Augustin, 1999). ECs originating from the embedded spheroids invade the gel to form capillary-like structures. Spheroids, generated from human umbilical-vein ECs (HUVECs), were embedded into type-I-collagen gels and stimulated with VEGF, SB-431542 or their combination for 24 hours (Fig. 3). In the absence of stimulation, hardly any EC sprouting was observed. Stimulation with different amounts of VEGF (10-50 ng/ml) dramatically increased the length and the number of sprouts (Fig. 3A). The addition of SB-431542 (1-10 μM) induced the formation of sprouting in a dose-dependent manner, but to a lesser extent compared with VEGF (Fig. 3B). Thus, both VEGF and SB-431542 promote EC sprouting. Interestingly, the addition of a VEGFR2-kinase inhibitor (PTK787) (Wood et al., 2000) blocked both VEGF- and SB-431542-induced sprouting (Fig. 3D). Because PTK787 can inhibit the stimulatory effect of SB-431542 on EC sprouting, we wondered whether SB-431542 enhances VEGFR2 phosphorylation. We observed no effect of SB-431542 on basal and VEGF-induced VEGFR2 phosphorylation levels or on the VEGF-induced activation of downstream pathways such as ERK and p38 MAP kinases (data not shown). Treatment of HUVECs with SB-431542 decreased basal levels of phosphorylated Smad2, indicating the presence of TGFβ-like factors in media supplements or active TGFβ secreted by HUVECs. VEGF had no effect on the levels of Smad2 phosphorylation (data not shown). Taken together, our results indicate that SB-43152 and VEGF do not influence immediate VEGFR2- or ALK5-induced responses, respectively.
Although stimulation of the spheroids with VEGF (50 ng/ml) and SB-431542 (10 μM) further increased sprouting (Fig. 3C), this effect was additive and not synergistic. Therefore, we hypothesized that the concentrations of VEGF and SB-431542 are near-to-plateau levels, and we analyzed the effect of sub-optimal concentrations of both VEGF and the ALK5-kinase inhibitor. Stimulation of EC spheroids with 1 ng/ml VEGF or 0.2 μM SB-431542 alone resulted in a small induction of sprouting compared with control (Fig. 4A,B). Interestingly, the combination of low levels of VEGF and SB-431542 synergistically enhanced EC sprouting (Fig. 4A,B). Similar results were obtained when the TGFβ-type-I-receptor-kinase inhibitor LY-2157299 was used. The combination of sub-optimal concentrations of LY-2157299 and VEGF resulted in enhanced sprouting compared with VEGF or the inhibitor alone (Fig. 4C,D).
Because SB-431542 interferes with signaling not only of the TGFβ type-I receptor ALK5, but also with the activity of the Activin and Nodal type-I receptors ALK4 and ALK7, we investigated whether the effects seen are due to inhibition of TGFβ or antagonism of Activin-Nodal signaling. We therefore used the 1D11 TGFβ-neutralizing antibody (Dasch et al., 1989). EC spheroids were stimulated with a low concentration of VEGF in the presence or absence of the TGFβ-neutralizing antibody or an isotype-matched control antibody (Fig. 4E). The isotype-matched control antibody had no effect on basal or VEGF-induced sprouting. Addition of the TGFβ-neutralizing antibody did not induce EC sprouting. However, the combination of TGFβ-neutralizing antibody with VEGF significantly induced EC sprouting compared with VEGF or TGFβ-blocking antibody alone (Fig. 4E). As expected, ectopic expression of a constitutively active form of ALK5 receptor (caALK5) exerted the opposite response of SB-431542 treatment and inhibited VEGF-induced HUVEC sprouting in the 3D spheroid system (supplementary material Fig. S1A). Moreover, caALK5 overexpression inhibited basal cord formation of mouse embryonic endothelial cells (MEECs) (supplementary material Fig. S1B). Taken together, our results suggest that inhibition of the TGFβ signaling pathway significantly enhances VEGF-induced EC sprouting in vitro.
VEGF and ALK5 inhibitor promote EC migration
To further investigate the mechanisms by which VEGF and SB-431542 exert their synergistic effect on angiogenesis, we analyzed their effects on EC migration. To study the effect of VEGF and SB-431542 on migration, serum-starved monolayers of HUVECs were wounded by scratching and were stimulated for 6 hours. There was no effect on migration when cells were stimulated by VEGF or the ALK5-kinase inhibitor alone. However, the combination of VEGF and SB-431542 significantly induced EC migration (Fig. 5A,B). Consistent with these results, overexpression of caALK5 in MEECs resulted in reduced migration, using the Transwell migration assay, and invasion, using a Transwell invasion assay with Matrigel coating (Goumans et al., 2002) (supplementary material Fig. S2A,B).
Transcriptional profiling of the VEGF+SB-431542-induced EC response
Trying to unravel the mechanistic basis of the VEGF and SB-431542 synergistic effects on angiogenesis (EC sprouting), we analyzed expression of a number of genes essential to EC function by quantitative real-time PCR. We used a highly sensitive commercial PCR-based array system containing up to 84 genes related to EC function. From the genes analyzed, the expression of 20 of them was significantly upregulated when cells were stimulated by the combination of VEGF and SB-431542, but not by VEGF or SB-431542 alone (Table 1). In addition, VEGF+SB-431542 stimulation resulted in the downregulation of 30 genes. Interestingly pro- and anti-apoptotic genes were differentially regulated following VEGF+SB-431542 stimulation. There was an upregulation in expression of the anti-apoptotic gene Bcl2 and a significant downregulation of pro-apoptotic genes, such as annexin A5, Bax and caspase 6 (Table 1), suggesting that VEGF and SB-431542 induce pathways that promote EC survival. VEGF and SB-431542 synergistically induced the expression of several other genes implicated in angiogenesis, such as angiotensin receptor I, ACE, CCL5, IL3 and IL7 (Table 1). Integrins have been shown to play an important role in EC migration and survival, as well as in capillary sprouting during angiogenesis. In line with this notion, VEGF+SB-431542 stimulation synergistically induced α5 integrin and β3 integrin mRNA expression. To verify these results, we performed quantitative PCR analysis using PCR primer sets different from those used in the array (supplementary material Fig. S3A). Similar results were obtained when mRNA was isolated from EC spheroids embedded in collagen after VEGF and/or SB-431542 stimulation (supplementary material Fig. S3B).
|.|| Fold difference compared with control |
|Gene .||VEGF .||SB .||VEGF+SB .||P-value* .|
|.|| Fold difference compared with control |
|Gene .||VEGF .||SB .||VEGF+SB .||P-value* .|
P-value of VEGF+SB-stimulated versus untreated control cells. SB, SB-431542
Crucial role of α5 integrin in VEGF+SB-431542-induced angiogenesis
Stimulation of VEGF and inhibition of the TGFβ type-I receptor kinase significantly induces integrins α5 and β3 mRNA expression. Interestingly, pretreatment of HUVECs with cyclohexamide (inhibitor of protein synthesis) diminished the synergistic effect of VEGF+SB-431542 on α5-integrin expression (supplementary material Fig. S4). Those results indicate that the synergistic effect of VEGF and SB-431542 on integrin expression is not direct, but it requires de novo protein synthesis.
To investigate whether integrins α5 and β3 have a functional role in the synergistic effects of VEGF and SB-431542 on capillary sprouting, we studied the loss-of-function effects using an RNA-interference approach. HUVECs were transfected with siRNA oligonucleotides for α5 integrin and β3 integrin or with control siRNA and subsequently seeded to form spheroids, which were then embedded in collagen. In α5-integrin- and β3-integrin-siRNA-transfected cells, the levels of α5-integrin and β3-integrin RNA expression decreased on average 50% (data not shown). Quantification of sprouting demonstrated a significant decrease in VEGF+SB-431542-induced sprout length in the α5- and β3-integrin-siRNA-transfected spheroids compared with control siRNA (Fig. 6A). It is well recognized that integrin-mediated signaling plays a crucial role in angiogenesis. To exclude the possibility that downregulation of α5 integrin or β3 integrin results in a general block in angiogenesis, we tested the effect of α5- and β3-integrin siRNA on basic fibroblast growth factor (bFGF)- and VEGF (high concentration)-induced sprouting in the spheroid assay. Whereas α5-integrin downregulation diminished the VEGF+SB-431542-induced sprouting, it had no effect on bFGF- or high-dose-VEGF-induced capillary formation (Fig. 6A). Interestingly, downregulation of β3 integrin by siRNA affected VEGF+SB-431542- as well as bFGF- or high-dose-VEGF-induced EC sprouting (Fig. 5A). Moreover, α5-integrin downregulation had no effect on VEGF- (1 ng/ml) or SB-431542- (0.2 μM) induced EC sprouting (Fig. 6B).
To further substantiate the role of α5 integrin in the synergistic effect of VEGF and SB-431542 on angiogenesis, we made use of an α5-integrin-neutralizing antibody. Addition of α5-integrin function-blocking antibody inhibited the synergistic effect of VEGF+SB-431542 on EC sprouting (Fig. 6C). However, α5-integrin-neutralizing antibody had no effect on high-dose-VEGF-induced capillary formation (Fig. 6D). Our results suggest that α5 integrin is necessary for VEGF+SB-431542-mediated synergistic induction of EC sprouting in the 3D EC spheroid assay.
VEGF and ALK5-kinase inhibitor synergistically enhance in vivo angiogenesis in a Matrigel-plug assay in mice
In order to characterize the effect of inhibition of the ALK5 receptor in VEGF-induced angiogenesis, we analyzed the in vivo angiogenesis response by placing Matrigel plugs without and with supplements (LY-2157299 and/or VEGF and bFGF) under the skin of mice. Because VEGF alone did not induce angiogenesis in the in vivo Matrigel-plug assay (data not shown), we used a combination of VEGF and bFGF. Vascularization was assayed by CD31 staining of sections from the recovered plugs (Fig. 7B,C). Matrigel plugs without any stimulus showed no vascularization. Addition of LY-2157299 or VEGF+bFGF combination significantly induced angiogenesis (Fig. 7A,B). Addition of LY-2157299 with VEGF+bFGF (VEGF+bFGF+LY) further significantly enhanced the blood-vessel formation (Fig. 7A). To corroborate the role of α5 integrin in VEGF+bFGF+LY synergistic effect on in vivo angiogenesis we examined the effect of the α5-integrin-neutralizing antibody in the Matrigel-plug assay. Whereas addition of α5-integrin-neutralizing antibody but not a control antibody inhibited VEGF+bFGF+LY-induced angiogenesis, it had no significant effect on VEGF+bFGF-induced angiogenesis. These results illustrate that inhibition of TGFβ-ALK5 signaling in vivo can potentiate the pro-angiogenic effect of VEGF and bFGF, which is critically dependent on the induction of α5-integrin signaling.
Here we show that inhibition of TGFβ type-I receptor (ALK5) and VEGF synergistically promotes angiogenesis. Treatment with the ALK5 inhibitors SB-43152 or LY-2157299 strongly enhanced VEGF-induced EC capillary formation and EC sprouting. Similar results were obtained using a TGFβ-neutralizing antibody (Dasch et al., 1989), indicating that inhibition of TGFβ, and not of Activin-Nodal signaling, is likely to cooperate with VEGF in inducing angiogenesis. Addition of the VEGFR2-kinase inhibitor PTK787 (Wood et al., 2000) blocked not only the VEGF- but also the SB-431542-induced EC sprouting, suggesting that inhibition of TGFβ signaling potentiates autocrine VEGF signaling in ECs. Importantly, the TGFβ-receptor-kinase inhibitor promoted basal and VEGF+bFGF-induced angiogenesis in vivo in a Matrigel-plug assay. Conversely, ectopic expression of caALK5 potently inhibited all VEGF-induced pro-angiogenic responses of ECs.
Recently it was reported that inhibition of TGFβ antagonized the pro-angiogenic response on human normal dermal microvascular ECs (MVECs) in vitro (Serrati et al., 2008). There are several explanations for the discrepancy with our results. First, the pro-angiogenic effects of TGFβ were inhibited using TGFβ antagonistic peptides derived from the TGFβ type-III receptor sequence. By contrast, we used pharmacological inhibitors of the TGFβ and/or Activin receptors and a pan-TGFβ-neutralizing antibody in combination with VEGF. TGFβ type-III receptor facilitates TGFβ signaling by enhancing the binding of ligand to TβRII (Lopez-Casillas et al., 1993). It was recently shown that the TGFβ type-III receptor can bind multiple members of the BMP subfamily, including BMP2, BMP4 and BMP7, and thereby potentiate BMP signaling. BMP-2, BMP4 and BMP7 have been shown to induce angiogenesis (Boyd et al., 2007; Deckers et al., 2001; Langenfeld and Langenfeld, 2004; Raida et al., 2005; Valdimarsdottir et al., 2002). Consequently, it is possible that the peptide antagonists used by Serrati and co-workers inhibit endogenous BMP signaling (Serrati et al., 2008). Second, ECs of different origin were used. Seratti used MVECs, whereas we used HUVECs. These ECs might respond differently to TGFβ signaling owing to, e.g. expression of different receptors or different levels of TGFβ production. We show that treatment of HUVEC spheroids with the TGFβ-receptor-kinase inhibitor (or TGFβ-neutralizing antibody) resulted in decreased basal Smad2 phosphorylation and increased sprouting, suggesting that endogenous TGFβ signaling or TGFβ present in media supplements or produced by the cells inhibits VEGF signaling in HUVECs.
Transcriptional profiling revealed that the VEGF+SB-431542 stimulation of HUVECs synergistically regulates a number of genes involved in angiogenesis. Our results showed that VEGF+SB-431542 stimulation of ECs resulted in a dramatic decrease in the expression of pro-apoptotic genes and an increase in that of anti-apoptotic genes. TGFβ was shown to induce apoptosis of ECs via autocrine and/or paracrine stimulation of VEGF expression and signaling by VEGFR2 via downstream activation of p38 (Ferrari et al., 2006). We observed no effects of SB-431542 on VEGF-induced ERK and p38 phosphorylation (data not shown). Thus, on the basis of our results, we suggest that stimulation of ECs with VEGF and SB-431542 protects ECs from apoptosis and enhances their survival and proliferation. This might explain the dramatic induction of EC-sheet formation we observed upon VEGF+SB-431542 challenge in the ex vivo metatarsal assay.
α5 integrin, a cell-surface receptor for fibronectin, is one of the genes that is synergistically upregulated by VEGF+SB-431542 co-stimulation. We confirm the importance of this upregulation in VEGF+SB-431542-induced EC sprouting because genetic ablation and function-neutralizing antibodies of α5 integrin inhibit VEGF+SB-431542-induced EC sprouting in vitro and in vivo. Our results underline the important role of α5 integrin demonstrated before in blood-vessel development; α5-integrin-null embryos exhibit abnormal blood-vessel formation and a lower-complexity vascular network (Francis et al., 2002). Furthermore, α5β1-integrin function-blocking antibody was shown to inhibit HUVEC tube formation in vitro (Ramakrishnan et al., 2006), and to inhibit tumor neovascularization and growth in animal models (Kim et al., 2000).
VEGF and TGFβ signaling play a central role in tumor angiogenesis and thus in tumor development and metastasis. Several therapeutic strategies targeting TGFβ signaling have been shown to prevent the growth and metastasis of certain cancers (Yingling et al., 2004). Interestingly, a recent study using low doses of the TβRI-kinase inhibitor (LY364947) in experimental tumors decreased VSMC coverage of the tumor endothelium and promoted the accumulation of anticancer drugs in the tumor tissue (Kano et al., 2007). Our results suggest that inhibition of TGFβ signaling renders the ECs more sensitive to VEGF-induced sprouting in vitro and in vivo. Therefore, anti-TGFβ-based therapeutic strategies must be carefully considered before administration of TGFβ antagonists because there might be adverse effects, including the induction of tumor angiogenesis. A better treatment modality, as suggested by this study, is combining VEGF- and TGFβ-pathway inhibitors to inhibit tumor-cell metastasis and angiogenesis.
In conclusion, we investigated the effect of TGFβ-type-I-receptor inhibitor(s) or TGFβ-neutralizing antibody on VEGF-induced EC sprouting. Our results suggest that combining VEGF and inhibition of TGFβ-ALK5 signaling synergistically promotes angiogenesis in vitro and in vivo by inducing a cascade of expression of genes that play important roles in EC survival and in angiogenesis. Functional studies revealed that the induction and function of α5 integrin is a key determinant in VEGF+SB-431542-induced angiogenesis.
Materials and Methods
Recombinant proteins, inhibitors and antibodies
The VEGF165 isoform was purchased from R&D Systems and bFGF from Peprotech. SB-431542 was purchased from Tocris Biotrend, LY-2157299 from Calbiochem and PTK787 from Novartis. α5-integrin-neutralizing antibody was purchased from BD Biosciences, the TGFβ-neutralizing antibody and isotype control obtained from Genzyme, and the Fc domain of IgG1 (MOPC-21) from Bio Express, West Lebanon, NH.
Ex vivo metatarsal angiogenic assay
All animal experiments were approved by the local animal ethics committee. Metatarsals from 17-day-old mouse fetuses were dissected as described earlier (van der Pluijm et al., 1991). Six bones per experimental group were transferred to 24-well tissue-culture plates containing α-MEM (Gibco), 10% FBS and penicillin/streptomycin (PS), and allowed to adhere for 4 days. Then, medium was replaced by fresh medium containing the stimuli. Cultures were fixed 7 days after stimulation and vessel formation was visualized by anti-CD31 staining (Deckers et al., 2001).
HUVECs cells were cultured in Medium 199 with Earle's salt and L-glutamine (Gibco), 10% FCS, heparin (LEO pharma), bovine pituitary extract (Gibco) and PS on plates coated with 1% gelatin, at 37°C, 5% CO2. HUVECs were used up to passage 4. Experiments were confirmed with HUVECs from different donors. MEECs were cultured in DMEM (Gibco) supplemented with 10% FCS and PS on 0.1% gelatin-coated plates, at 37°C, 5% CO2.
3D-culture spheroid assay
HUVECs (400 cells per spheroid) were suspended in Medium M199 containing Earle's salt and L-glutamine, 10% FBS, heparin, bovine pituitary extract, PS and seeded in non-adherent round-bottom 96-well plates. After 24 hours, spheroids were embedded into collagen and stimulated with corresponding stimuli in the presence or absence of inhibitors or neutralizing antibodies for another 24 hours. EC sprouts were measured by Olympus Analysis software.
Migration scratch assay
HUVECs were seeded in six-well plates coated with 1% gelatin and allowed to grow to confluence. After serum starvation, monolayers were wounded with three scratches and medium was replaced by fresh medium containing stimuli. Cell migration was measured in five areas per well directly after wounding and 6 hours later by automated image analysis using ImageJ software.
Male 7- to 8-week-old C57BL/6 mice (Charles River Laboratories, Sulzfeld, Germany) were injected subcutaneously near their abdominal midline with 0.4 ml of Matrigel basement membrane, high concentration (BD Biosciences, San Jose, CA) combined with either PBS, VEGF (300 ng/ml) and bFGF (700 ng/ml), and/or LY-2157299 (0.1 μM), in the presence or absence of α5-integrin-neutralizing antibody (20 μg/ml) or Fc control protein (20 μg/ml). Groups of four plugs were injected for each treatment and the experiment was repeated twice. Seven days later Matrigel plugs were removed, fixed in formalin and embedded in paraffin. Sections were subjected to histological analysis with eosin. Sections were deparaffinized. Quenching of endogenous peroxidase activity was done using 0.3% H2O2 in methanol for 20 minutes at room temperature (RT), followed by antigen retrieval using citrate buffer and blocking with 1% BSA in PBS for 1 hour at RT. The primary antibody against CD31 (1:1000, Santa Cruz Biotechnologies, Santa Cruz, CA) was incubated in 1% BSA in PBS overnight at RT. Biotin-conjugated secondary antibodies were applied followed by amplification using the strep-AB-complex/HRP (DAKO, Hamburg, Germany). Finally, diaminobenzidine substrate (Sigma) was added to visualize peroxidase activity. The area covered by CD31-positive staining was quantified with image analysis.
Cell transfection and RNA interference
Cells were seeded in six-well plates and the following day were transiently transfected with control siRNA or siRNA against α5 integrin and β3 integrin, purchased from Dharmacon, according to the manufacturer's instructions. One day after transfection, cells were trypsinized and seeded for spheroid formation or used for RNA isolation.
RNA isolation and EC PCR array
Total DNA-free cellular RNA was extracted with RNeasy kit (Macherery-Nagel) according to the manufacturer's instruction. RNA from HUVECs grown in monolayers that were either non-treated or treated with VEGF, SB-431542 or VEGF+SB-431542 for 24 hours was isolated from three independent biological experiments and separately analyzed on the array. A commercial EC PCR-array (purchased from Superarray) was used to investigate gene expression profiling of selected angiogenesis-related genes involved in EC function (http://www.sabiosciences.com/rt_pcr_product/HTML/PAHS-015A.html). The experiment was performed in triplicate. The manufacturer's instructions were strictly followed (http://www.sabiosciences.com/). Gene expression levels were determined by using data analyzer template provided by Superarray (http://www.sabiosciences.com/pcrarraydataanalysis.php), using GAPDH, β-actin and ribosomal protein L13a as reference. The non-stimulated condition was set to 1.
HUVECs or MEECs growing in 70-80% confluent monolayers were infected with an adenovirus expressing either caALK5 or lacZ with an MOI of 500. At 48 hours after infection, cells were trypsinized and re-seeded to be used in different assays.
EC cord formation
Matrigel Basement Membrane Matrix Growth Factor Reduced (Becton Dickinson) was added at 50 μl to each well of a 96-well plate and allowed to polymerize for 1 hour at 37°C. Cells were removed from culture by trypsinization and resuspended at 30,000 cells/ml. 100 μl cell suspension were plated in each well in triplicates and plates incubated for 48 hours. Pictures were acquired with a phase-contrast microscope in four different fields. The length of branches was quantified using automated image analysis using the Olympus Analysis software.
Transwell migration and invasion assays
Transwell migration was performed in 24-well plates with filter inserts of a pore size of 0.8 μm (Costar). For the Transwell invasion assay, filters were coated with Matrigel. 30,000 cells were seeded in the upper chamber. Experiments were done in triplicates. Cells were fixed after 20 hours with 4% paraformaldehyde and stained with 0.1% crystal violet. Pictures of the filters were acquired with phase-contrast microscopy at 10 magnification. Three fixed positions were imaged of each membrane and the number of cells was counted.
RNA isolation and quantitative PCR analysis
RNA from HUVECs growing in monolayers or EC spheroids embedded in collagen [non-treated, or VEGF- (1 ng/ml), SB-431542- (0.2 μM) or VEGF+SB-431542-treated for 24 hours) was isolated from three independent biological experiments with RNeasy kit (Macherery-Nagel) and subjected to cDNA synthesis with RevertAid H Minus first strand cDNA synthesis kit (Fermentas) according to the manufacturer's instruction. Expression of α5 integrin (ITGα5) and house-keeping gene acidic ribosomal phosphoprotein (ARP) were analyzed using the following primers: ITGα5 forward: 5-ATACTCTGTGGCTGTTGGTGAATTC-3; ITGα5 reverse: 5-ATTAAGGATGGTGACATAGCCGTAA-3; ARP forward: 5-CACCATTGAAATCCTGAGTGATGT-3; ARP reverse: 5-TGACCAGCCGAAAGGAGAAG-3. Taqman PCR reactions were performed using the ABI prism HT7900 sequence-detection system (Applied Biosystem). All samples were plated in duplicates. Gene expression levels were determined with the comparative ΔCt method using ARP as reference and the non-stimulated condition was set to 1.
HUVECs growing in monolayers were either untreated or pretreated with 5 μg/ml cyclohexamide for 1 hour, and subsequently unstimulated or treated with 1 ng/ml VEGF, 0.2 μM SB-431542 or a combination of both VEGF and SB-431542 for 24 hours. RNA was isolated and subjected to cDNA synthesis followed by quantitative real-time PCR analysis.
All results are expressed as the mean ± s.d. Statistical differences were examined by two-tailed Student's t-test and P≤0.05 was considered to be statistically significant (in the figures, *P≤0.05 and **P≤0.01).
This study was supported by grants from the Centre of Biomedical Genetics, Dutch Cancer Society (RUL 2005-3371), FP6 EC Integrated Project Angiotargeting 504743, EC STREP Tumor-Host-Genomics and the Ludwig Institute for Cancer Research. We thank Scot Lonning of Genzyme for TGFβ-neutralizing antibody, Maj Petersen, Eric Danen, Lucas Hawinkels and Gabri van der Pluijm for discussion, and Henny Bloys and Midory Thorikay for excellent technical assistance.