Dagenbach, E. M. and Endow, S. A. (2004). A new kinesin tree. J. Cell Sci.117, 3-7.

The neck analysis entries in Table 1 were not aligned correctly in both the print and online versions of this paper. The corrected Table 1 is shown below. We apologise for any inconvenience caused.

Table 1.

Analysis of previously unclassified kinesin proteins for assignment to groups


Group

Proteins compared to

Protein

Motor position*

Motor sequence ID (%)

Predicted coiled-coil position

Notes

Neck analysis
At1
 
AtF3K23.6
 
AtF24M12.190
 
N
 
66.9
 
380-425
 

 

 

 

 
AtK1L20.9
 
N
 
67.1
 
383-434
 

 

 

 

 
AtT19F6.160
 
N
 
66.6
 
329-360
 

 

 

 

 
AtMDH9.19
 

 
52.9
 
None
 

 

 

 

 
AtF19H22.50
 
N
 
76.6
 
336-365
 

 

 

 

 
AtF7K15.60
 
N
 
55.0
 
385-434
 

 

 

 

 
AtF15H18.12
 
N
 
44.9
 
418-464
 
1 aa missing from alignment
 

 

 

 
AtF3K23.6
 
N
 
100
 
364-393
 

 

 
At2
 
AtF3K23.14
 
AtF19H22.150
 
N
 
73.8
 
489-580
 

 

 

 

 
AtT21B14.15e
 
N
 
74.2
 
385-426
 

 

 

 

 
AtF15M7.20
 
N
 
72.0
 
394-432
 

 

 

 

 
AtF8K7.17
 
N
 
76.7
 
394-474
 

 

 

 

 
AtF3K23.14
 
N
 
100
 
422-513
 

 

 
Unnamed At group
 
AtF28P10.150
 
AtF22M8.8
 
N
 
65.7
 
423-452
 

 

 

 

 
AtF5011.15
 
N
 
69.7
 
466-495
 

 

 

 

 
AtF28P10.150
 
N
 
100
 
473-524
 

 

 
Unnamed At group
 
AtPAKRP1
 
AtMAL21.18
 
N
 
44.1
 
421-453
 

 

 

 

 
AtMDB19.17
 
N
 
89.6
 
439-468
 
Phragmoplast-associated
 

 

 

 
AtPAKRP1
 
N
 
100
 
1044-1073
 
Phragmoplast-associated
 

 
Unnamed At group
 
AtT15B3.190
 
AtMGD8.20
 
N
 
64.9
 
494-527
 

 

 

 

 
AtK13E13.17
 
N
 
64.1
 
771-805
 

 

 

 

 
AtT15B3.190
 
N
 
100
 
578-609
 

 

 
Unnamed At group
 
AtT1E22.130
 
AtMRO11.5
 
N
 
34.5
 
None
 
Grouped by bootstrap
 

 

 

 
AtT1E22.130
 

 
100
 
None
 

 

 
BimC
 
AnBimC
 
PfMAL3P6.13
 
N
 
37.0
 
None
 

 

 

 

 
HsKIF8
 

 
(53.5)
 

 
Partial sequence
 

 

 

 
AtKRP125a
 
N
 
50.1
 
(438-467) P=0.41
 

 

 

 

 
AtKRP125b
 
N
 
50.8
 
406-477
 
GenBank identifies as cytokinesis motor
 

 

 

 
AtKRP125c
 
N
 
53.3
 
438-518
 
GenBank identifies as spindle motor
 

 

 

 
AtF16L2.60
 
N
 
50.1
 
433-523
 

 

 
CENP-E
 
HsCENP-E
 
DmCana
 
N
 
40.4
 
487-536
 
Kinetochore motor (Yucel et al 2000)
 

 

 

 
DmCmeta
 
N
 
48.1
 
333-374
 
Kinetochore motor (Yucel et al 2000)
 

 

 

 
AtF14P13.22
 
N
 
49.3
 
364-410
 
GenBank identifies as putative kinesin-like centromere protein
 

 

 

 
AtZCF125
 
N
 
51.0
 
359-414
 

 

 
Chromokinesin/KIF4
 
HsKIF4
 
HsNYREN62
 
N
 
50.7
 
467-509
 
Group supported by neighbor-joining bootstrap value of 95% and high % sequence ID to HsKIF4
 

 

 

 
AtMSL3.5
 
N
 
49.5
 
581-615
 
 
 

 

 
AtMCA23.16
 
N
 
47.2
 
539-573
 
 
 

 

 
AtF11C1.80
 
N
 
50.4
 
411-443
 
 
 
Unnamed Hs group
 
HsKIAA1236
 
HsKIAA1236
 

 
100
 
None
 

 

 

 

 
HsLOC343489
 

 
54.4
 
None
 
May not be full-length sequence
 

 
KHC
 
DmKHC
 
AtMAA21.110
 
N
 
40.6
 
374-418
 
Bootstrap values do not support placement in KHC group
 

 
Kip3
 
ScKip3
 
PfMAL3P7.1
 
N
 
41.9
 
594-629
 
  

 

 
HsKIF18
 
N
 
47.7
 
None
 
Bootstrap values do not support placement in Kip3 group   

 

 
AtF25116.11
 
N
 
44.7
 
502-540
 
 
 

 

 
DmK1p67A
 
N
 
43.6
 
362-398
 
 
 

 

 
DmKIF19A
 
N
 
39.0
 
550-598
 
 
 

 

 
HsFLJ37300
 
N
 
45.4
 
420-451
 
DmKIF19A listed in alignment as DmK1p19A
 

 

 

 
PfMAL1P2.n
 
N
 
46.6
 
1412-1442
 
 
 
KRP85/95
 
CeOsm3
 
HsKIAA1405
 
N
 
58.3
 
368-411
 
Group supported by neighbor-joining bootstrap value of 100% and high % sequence ID to CeOsm3
 

 

 

 
DmKIF3C
 
N
 
50.9
 
(351-380) P=0.44
 
 
 
MCAK/ KIF2
 
HsMCAK
 
HsK1p17q22
 
Central
 
67.7
 
None P<0.4
 

 

 

 

 
DmK1p59D
 
Central
 
61.7
 
None
 

 

 

 

 
AtMGL6.9
 
Central
 
52.1
 
705-741
 

 

 

 

 
AtMSL1.9
 
Central
 
53.3
 
596-627
 

 

 

 

 
PfL2165w
 
(Central)
 
45.3
 
None
 
Motor towards N terminus
 

 
MKLP1
 
DmPAV
 
HsK1pMPP1
 
N
 
33.2
 
568-607
 
Bootstrap values and % sequence ID to DmPav do not support placement in MKLP1 group
 

 

 

 
HsRabK6
 
N
 
27.0
 
680-718
 
 
 

 

 
AtT20H2.17
 
(N)
 
13.5
 
(839-868) P=0.49
 
 
 
Unc104/ KIF1
 
CeUnc104
 
HsKIF1B
 
N
 
70.6
 
504-533
 

 

 

 

 
HsGAKIN
 
N
 
58.5
 
612-644
 

 

 

 

 
HsRBKIN1
 
N
 
57.5
 
391-420
 

 

 

 

 
HsKIF16B
 
N
 
49.7
 
None P<0.4
 
Sequence only ∼65 aa longer than motor domain, may not be full length
 

 
C-Terminal motor   AtKATD   AtT12M4.14   N   67.3   750-788   Coiled-coil after motor domain   + + + G+  

 

 

 

 

 

 
Groups with AtKatD by bootstrap
 
KICNIKSNECITGS
 
   AtF15H18.10   C   62.1   433-523    ++L N + +L+GN  

 

 

 

 

 

 

 
NQKLFNELQELKGN
 
   AtF25P22.28   C   63.8   352-444    ++L N + +L+GN  

 

 

 

 

 

 

 
NRKLFNELQELKGN
 
   AtF24D7.17   C   62.1   285-384    + L+N V +L+GN  

 

 

 

 

 

 

 
NRRLYNEVQELKGN
 
   AtK1013.11   C   61.5   276-331    + L+N V +L+GN  

 

 

 

 

 

 

 
NRRLYNEVQELKGN
 
   AtF14P13.9   Central   55.8   None   Neck present   ++L+N V DL+GN  

 

 

 

 

 

 
Groups with AtKatD by bootstrap
 
NRKLYNMVQDLKGN
 
   HsKIFC2   C   37.8   270-320   C-terminal motor   + +L+GN  

 

 

 

 

 

 

 
PAGCPGRLPELKGN
 
   AtT9I22.5   C   39.9   388-430   C-terminal motor   RKEL+N + + +GN  

 

 

 

 

 

 
Neck present
 
RKELYNHIQETKGN
 
   AtF15A18.10   N   51.9   375-410   Coiled-coil after motor domain   RK L+N V++L+GN  

 

 

 

 

 

 
Neck present
 
RKRLYNEVIELKGN
 
   AtT9N14.6   C   52.0   341-373    RKEL+N +++L+GN  

 

 

 

 

 

 

 
RKELYNKILELKGN
 
   AtF15F15.20   C   42.2   35-78   C-terminal motor   RK++ N ++D +G+  

 

 

 

 

 

 
Neck present
 
RKQVLNKIIDTKGS
 
   AtF12B17.180   C   36.4   57-92   C-terminal motor   +K L N ++ +GN  

 

 

 

 

 

 
Neck present
 
KKRLFNDLLTAKGN
 
   AtMNA5.20   C   33.8   52-85   C-terminal motor   +K L N ++ +GN  

 

 

 

 

 

 
Neck present
 
KKRLFNDLLTTKGN
 
   AtC17L7.110   C   44.5   383-413   C-terminal motor   RK+LHNT+++L+GN  

 

 

 

 

 

 
Neck present
 
RKKLHNTILELKGN
 

Group

Proteins compared to

Protein

Motor position*

Motor sequence ID (%)

Predicted coiled-coil position

Notes

Neck analysis
At1
 
AtF3K23.6
 
AtF24M12.190
 
N
 
66.9
 
380-425
 

 

 

 

 
AtK1L20.9
 
N
 
67.1
 
383-434
 

 

 

 

 
AtT19F6.160
 
N
 
66.6
 
329-360
 

 

 

 

 
AtMDH9.19
 

 
52.9
 
None
 

 

 

 

 
AtF19H22.50
 
N
 
76.6
 
336-365
 

 

 

 

 
AtF7K15.60
 
N
 
55.0
 
385-434
 

 

 

 

 
AtF15H18.12
 
N
 
44.9
 
418-464
 
1 aa missing from alignment
 

 

 

 
AtF3K23.6
 
N
 
100
 
364-393
 

 

 
At2
 
AtF3K23.14
 
AtF19H22.150
 
N
 
73.8
 
489-580
 

 

 

 

 
AtT21B14.15e
 
N
 
74.2
 
385-426
 

 

 

 

 
AtF15M7.20
 
N
 
72.0
 
394-432
 

 

 

 

 
AtF8K7.17
 
N
 
76.7
 
394-474
 

 

 

 

 
AtF3K23.14
 
N
 
100
 
422-513
 

 

 
Unnamed At group
 
AtF28P10.150
 
AtF22M8.8
 
N
 
65.7
 
423-452
 

 

 

 

 
AtF5011.15
 
N
 
69.7
 
466-495
 

 

 

 

 
AtF28P10.150
 
N
 
100
 
473-524
 

 

 
Unnamed At group
 
AtPAKRP1
 
AtMAL21.18
 
N
 
44.1
 
421-453
 

 

 

 

 
AtMDB19.17
 
N
 
89.6
 
439-468
 
Phragmoplast-associated
 

 

 

 
AtPAKRP1
 
N
 
100
 
1044-1073
 
Phragmoplast-associated
 

 
Unnamed At group
 
AtT15B3.190
 
AtMGD8.20
 
N
 
64.9
 
494-527
 

 

 

 

 
AtK13E13.17
 
N
 
64.1
 
771-805
 

 

 

 

 
AtT15B3.190
 
N
 
100
 
578-609
 

 

 
Unnamed At group
 
AtT1E22.130
 
AtMRO11.5
 
N
 
34.5
 
None
 
Grouped by bootstrap
 

 

 

 
AtT1E22.130
 

 
100
 
None
 

 

 
BimC
 
AnBimC
 
PfMAL3P6.13
 
N
 
37.0
 
None
 

 

 

 

 
HsKIF8
 

 
(53.5)
 

 
Partial sequence
 

 

 

 
AtKRP125a
 
N
 
50.1
 
(438-467) P=0.41
 

 

 

 

 
AtKRP125b
 
N
 
50.8
 
406-477
 
GenBank identifies as cytokinesis motor
 

 

 

 
AtKRP125c
 
N
 
53.3
 
438-518
 
GenBank identifies as spindle motor
 

 

 

 
AtF16L2.60
 
N
 
50.1
 
433-523
 

 

 
CENP-E
 
HsCENP-E
 
DmCana
 
N
 
40.4
 
487-536
 
Kinetochore motor (Yucel et al 2000)
 

 

 

 
DmCmeta
 
N
 
48.1
 
333-374
 
Kinetochore motor (Yucel et al 2000)
 

 

 

 
AtF14P13.22
 
N
 
49.3
 
364-410
 
GenBank identifies as putative kinesin-like centromere protein
 

 

 

 
AtZCF125
 
N
 
51.0
 
359-414
 

 

 
Chromokinesin/KIF4
 
HsKIF4
 
HsNYREN62
 
N
 
50.7
 
467-509
 
Group supported by neighbor-joining bootstrap value of 95% and high % sequence ID to HsKIF4
 

 

 

 
AtMSL3.5
 
N
 
49.5
 
581-615
 
 
 

 

 
AtMCA23.16
 
N
 
47.2
 
539-573
 
 
 

 

 
AtF11C1.80
 
N
 
50.4
 
411-443
 
 
 
Unnamed Hs group
 
HsKIAA1236
 
HsKIAA1236
 

 
100
 
None
 

 

 

 

 
HsLOC343489
 

 
54.4
 
None
 
May not be full-length sequence
 

 
KHC
 
DmKHC
 
AtMAA21.110
 
N
 
40.6
 
374-418
 
Bootstrap values do not support placement in KHC group
 

 
Kip3
 
ScKip3
 
PfMAL3P7.1
 
N
 
41.9
 
594-629
 
  

 

 
HsKIF18
 
N
 
47.7
 
None
 
Bootstrap values do not support placement in Kip3 group   

 

 
AtF25116.11
 
N
 
44.7
 
502-540
 
 
 

 

 
DmK1p67A
 
N
 
43.6
 
362-398
 
 
 

 

 
DmKIF19A
 
N
 
39.0
 
550-598
 
 
 

 

 
HsFLJ37300
 
N
 
45.4
 
420-451
 
DmKIF19A listed in alignment as DmK1p19A
 

 

 

 
PfMAL1P2.n
 
N
 
46.6
 
1412-1442
 
 
 
KRP85/95
 
CeOsm3
 
HsKIAA1405
 
N
 
58.3
 
368-411
 
Group supported by neighbor-joining bootstrap value of 100% and high % sequence ID to CeOsm3
 

 

 

 
DmKIF3C
 
N
 
50.9
 
(351-380) P=0.44
 
 
 
MCAK/ KIF2
 
HsMCAK
 
HsK1p17q22
 
Central
 
67.7
 
None P<0.4
 

 

 

 

 
DmK1p59D
 
Central
 
61.7
 
None
 

 

 

 

 
AtMGL6.9
 
Central
 
52.1
 
705-741
 

 

 

 

 
AtMSL1.9
 
Central
 
53.3
 
596-627
 

 

 

 

 
PfL2165w
 
(Central)
 
45.3
 
None
 
Motor towards N terminus
 

 
MKLP1
 
DmPAV
 
HsK1pMPP1
 
N
 
33.2
 
568-607
 
Bootstrap values and % sequence ID to DmPav do not support placement in MKLP1 group
 

 

 

 
HsRabK6
 
N
 
27.0
 
680-718
 
 
 

 

 
AtT20H2.17
 
(N)
 
13.5
 
(839-868) P=0.49
 
 
 
Unc104/ KIF1
 
CeUnc104
 
HsKIF1B
 
N
 
70.6
 
504-533
 

 

 

 

 
HsGAKIN
 
N
 
58.5
 
612-644
 

 

 

 

 
HsRBKIN1
 
N
 
57.5
 
391-420
 

 

 

 

 
HsKIF16B
 
N
 
49.7
 
None P<0.4
 
Sequence only ∼65 aa longer than motor domain, may not be full length
 

 
C-Terminal motor   AtKATD   AtT12M4.14   N   67.3   750-788   Coiled-coil after motor domain   + + + G+  

 

 

 

 

 

 
Groups with AtKatD by bootstrap
 
KICNIKSNECITGS
 
   AtF15H18.10   C   62.1   433-523    ++L N + +L+GN  

 

 

 

 

 

 

 
NQKLFNELQELKGN
 
   AtF25P22.28   C   63.8   352-444    ++L N + +L+GN  

 

 

 

 

 

 

 
NRKLFNELQELKGN
 
   AtF24D7.17   C   62.1   285-384    + L+N V +L+GN  

 

 

 

 

 

 

 
NRRLYNEVQELKGN
 
   AtK1013.11   C   61.5   276-331    + L+N V +L+GN  

 

 

 

 

 

 

 
NRRLYNEVQELKGN
 
   AtF14P13.9   Central   55.8   None   Neck present   ++L+N V DL+GN  

 

 

 

 

 

 
Groups with AtKatD by bootstrap
 
NRKLYNMVQDLKGN
 
   HsKIFC2   C   37.8   270-320   C-terminal motor   + +L+GN  

 

 

 

 

 

 

 
PAGCPGRLPELKGN
 
   AtT9I22.5   C   39.9   388-430   C-terminal motor   RKEL+N + + +GN  

 

 

 

 

 

 
Neck present
 
RKELYNHIQETKGN
 
   AtF15A18.10   N   51.9   375-410   Coiled-coil after motor domain   RK L+N V++L+GN  

 

 

 

 

 

 
Neck present
 
RKRLYNEVIELKGN
 
   AtT9N14.6   C   52.0   341-373    RKEL+N +++L+GN  

 

 

 

 

 

 

 
RKELYNKILELKGN
 
   AtF15F15.20   C   42.2   35-78   C-terminal motor   RK++ N ++D +G+  

 

 

 

 

 

 
Neck present
 
RKQVLNKIIDTKGS
 
   AtF12B17.180   C   36.4   57-92   C-terminal motor   +K L N ++ +GN  

 

 

 

 

 

 
Neck present
 
KKRLFNDLLTAKGN
 
   AtMNA5.20   C   33.8   52-85   C-terminal motor   +K L N ++ +GN  

 

 

 

 

 

 
Neck present
 
KKRLFNDLLTTKGN
 
   AtC17L7.110   C   44.5   383-413   C-terminal motor   RK+LHNT+++L+GN  

 

 

 

 

 

 
Neck present
 
RKKLHNTILELKGN
 
*

Position of motor domain in protein and/or relative to predicted coiled-coil.

Coiled-coil predicted by PAIRCOIL (P≥50%). Coiled-coil position is that closest to the end of the motor domain for N-terminal motors and the beginning of the motor domain for C-terminal motors.

Sequence similarity to DmNcd neck, the region adjacent to the beginning of the conserved motor domain (RKELHNTVMDLRGN; top); sequence at bottom from corresponding region of protein under analysis.