SETA (also known as CIN85 or Ruk) is an SH3-domain-containing adaptor protein that functions in several signalling pathways – for example, it regulates PI 3-kinase (PI3K) signalling by interacting with the PI3K regulatory subunit p85α and facilitates internalization of receptor tyrosine kinases by binding to the ubiquitin ligase Cbl. Oliver Bögler and co-workers have found that SETA has another string to its bow: it's involved in cell adhesion (see p. 2845). Using confocal microscopy, they observe that SETA co-localizes with actin along microfilaments and at focal adhesions, as well as with microtubules. The protein does not interact with cytoskeletal filaments directly; instead these interactions are mediated by the SETA partner AIP1, which the authors show binds strongly to actin and tubulin. Bögler and co-workers can also identify SETA and AIP1 in complexes containing focal adhesion kinase (FAK) and its relative PYK2. Moreover, they demonstrate that SETA and AIP1 can regulate cell adhesion in electrical cell-substrate sensor (ECIS) attachment assays and that AIP1 attenuates FAK/PYK2 phosphorylation. The authors thus conclude that SETA and AIP1 can regulate cell adhesion, suggesting they do so as part of a focal adhesion kinase regulatory complex.
SETA adapts to adhesion
SETA adapts to adhesion. J Cell Sci 15 July 2003; 116 (14): e1404. doi:
Download citation file:
Advertisement
Cited by
Call for papers - Cilia and Flagella: from Basic Biology to Disease
We are welcoming submissions for our upcoming special issue: Cilia and Flagella: from Basic Biology to Disease. This issue will be coordinated by two Guest Editors: Pleasantine Mill (University of Edinburgh) and Lotte Pedersen (University of Copenhagen). Submission deadline: 1 March 2025.
Biologists @ 100 - join us in Liverpool in March 2025
We are excited to invite you to a unique scientific conference, celebrating the 100-year anniversary of The Company of Biologists, and bringing together our different communities. The conference will incorporate the Spring Meetings of the BSCB and the BSDB, the JEB Symposium Sensory Perception in a Changing World and a DMM programme on antimicrobial resistance. Find out more and register your interest to join us in March 2025 in Liverpool, UK.
Principles and regulation of mechanosensing
Mechanics play a fundamental role in cell physiology and represent physical mechanisms which cells use to influence function from the molecular to tissue scale. In this Review, Stefano Sala and colleagues clearly define mechanosensing and mechanotransduction, illustrate various mechanosensing mechanisms and discuss methods that cells use to regulate these processes.
JCS-FocalPlane Training Grants
Early-career researchers - working in an area covered by JCS - who would like to attend a microscopy training course, please apply. Deadline dates for 2024 applications: 7 September (decision by week commencing 8 October 2024); 22 November (decision by week commencing 16 December).
HIV-1 assembly – when virology meets biophysics
Claire Lacouture and colleagues review mechanisms of assembly of the HIV-1 structural protein Gag from biophysical and biological perspectives. The researchers highlight how HIV-1 Gag hijacks host cell factors associated with cortical actin machinery to make new viral particles.