Sterk, L. M. T., Geuijen, C. A. W., van den Berg, J. G., Claessen, N.,Weening, J. J. and Sonnenberg, A.J. Cell Sci.115,1161-1173.

In the printed version of this article,Fig. 10 was incorrect. The correct figure is shown below.

Fig. 10.
Fig. 10. Expression of laminin-binding integrins on transfected K562 cells increases CD151 cell surface expression. Flow cytometric analysis of wild-type K562 cells endogenously expressing α5β1 and transfected K562 also expressing αvβ1, α3β1, α6β1, α6β4,α7X1β1 or α7X2β1. Solid lines in the left panels indicate staining with anti-integrin antibodies: anti-α5 mAb Sam-1 (K562 cells), anti-αv mAb 13C2 (K562-αv), anti-α3 mAb J143(K562-α3), anti-α6 mAb GoH3 (K562-α6), anti-β4 mAb 439-9B (K562-α6β4) and anti-α7 mAb CA25 (K562-α7BX1 and K562-α7BX2). Staining of cells with anti-CD151 antibodies is shown in the right panels. Solid lines represent staining of cells with mAb TS151R and broken lines with mAb P48. A negative control (dotted line) with secondary goat anti-mouse or rat IgG alone is shown in each panel. R represents the ratio of the mean fluorescence intensity of cells stained with mAb P48 and with secondary antibody alone. A three- to sixfold increase of CD151 expression is observed in transfected cells that ectopically express lamininbinding integrins. The TS151R epitope is masked in all cells expressing one of the laminin-binding integrins.
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Expression of laminin-binding integrins on transfected K562 cells increases CD151 cell surface expression. Flow cytometric analysis of wild-type K562 cells endogenously expressing α5β1 and transfected K562 also expressing αvβ1, α3β1, α6β1, α6β4,α7X1β1 or α7X2β1. Solid lines in the left panels indicate staining with anti-integrin antibodies: anti-α5 mAb Sam-1 (K562 cells), anti-αv mAb 13C2 (K562-αv), anti-α3 mAb J143(K562-α3), anti-α6 mAb GoH3 (K562-α6), anti-β4 mAb 439-9B (K562-α6β4) and anti-α7 mAb CA25 (K562-α7BX1 and K562-α7BX2). Staining of cells with anti-CD151 antibodies is shown in the right panels. Solid lines represent staining of cells with mAb TS151R and broken lines with mAb P48. A negative control (dotted line) with secondary goat anti-mouse or rat IgG alone is shown in each panel. R represents the ratio of the mean fluorescence intensity of cells stained with mAb P48 and with secondary antibody alone. A three- to sixfold increase of CD151 expression is observed in transfected cells that ectopically express lamininbinding integrins. The TS151R epitope is masked in all cells expressing one of the laminin-binding integrins.

Fig. 10.
Fig. 10. Expression of laminin-binding integrins on transfected K562 cells increases CD151 cell surface expression. Flow cytometric analysis of wild-type K562 cells endogenously expressing α5β1 and transfected K562 also expressing αvβ1, α3β1, α6β1, α6β4,α7X1β1 or α7X2β1. Solid lines in the left panels indicate staining with anti-integrin antibodies: anti-α5 mAb Sam-1 (K562 cells), anti-αv mAb 13C2 (K562-αv), anti-α3 mAb J143(K562-α3), anti-α6 mAb GoH3 (K562-α6), anti-β4 mAb 439-9B (K562-α6β4) and anti-α7 mAb CA25 (K562-α7BX1 and K562-α7BX2). Staining of cells with anti-CD151 antibodies is shown in the right panels. Solid lines represent staining of cells with mAb TS151R and broken lines with mAb P48. A negative control (dotted line) with secondary goat anti-mouse or rat IgG alone is shown in each panel. R represents the ratio of the mean fluorescence intensity of cells stained with mAb P48 and with secondary antibody alone. A three- to sixfold increase of CD151 expression is observed in transfected cells that ectopically express lamininbinding integrins. The TS151R epitope is masked in all cells expressing one of the laminin-binding integrins.
View largeDownload slide

Expression of laminin-binding integrins on transfected K562 cells increases CD151 cell surface expression. Flow cytometric analysis of wild-type K562 cells endogenously expressing α5β1 and transfected K562 also expressing αvβ1, α3β1, α6β1, α6β4,α7X1β1 or α7X2β1. Solid lines in the left panels indicate staining with anti-integrin antibodies: anti-α5 mAb Sam-1 (K562 cells), anti-αv mAb 13C2 (K562-αv), anti-α3 mAb J143(K562-α3), anti-α6 mAb GoH3 (K562-α6), anti-β4 mAb 439-9B (K562-α6β4) and anti-α7 mAb CA25 (K562-α7BX1 and K562-α7BX2). Staining of cells with anti-CD151 antibodies is shown in the right panels. Solid lines represent staining of cells with mAb TS151R and broken lines with mAb P48. A negative control (dotted line) with secondary goat anti-mouse or rat IgG alone is shown in each panel. R represents the ratio of the mean fluorescence intensity of cells stained with mAb P48 and with secondary antibody alone. A three- to sixfold increase of CD151 expression is observed in transfected cells that ectopically express lamininbinding integrins. The TS151R epitope is masked in all cells expressing one of the laminin-binding integrins.

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2002