Two storage compartments in cultured noradrenergic neurons derived from the superior cervical ganglion from fetal pig have been defined using sucrose density gradient centrifugation and electron microscopy: (1) large dense-cored vesicles (LDV) contain noradrenaline and dopamine-beta-hydroxylase (DbetaH); (2) small electron-lucent vesicles contain acetylcholine and p38 and represent the noradrenergic small synaptic vesicles (SSV); no small dense-cored vesicles (SDV) could be detected. Our results demonstrate that internalized LDV membrane constituents are retrieved into early endosomes, as shown by the colocalization of retrieved DbetaH with the endosomal markers Rab5 and HRP in sucrose density gradients and on confocal microscopical images. Recycling of the SSV membranes via an endosomal intermediate is also confirmed in noradrenergic neurons. Finally, colocalization of retrieved DbetaH and retrieved p38 in stimulated neurons indicates that the two sets of constituents intermix. These data provide the first experimental evidence for a common early endosome in which SSV and LDV membrane constituents are internalized after exocytosis and imply that endosomal sorting is an important process for the generation of different secretory vesicles in the noradrenergic nerve terminal.
Retrieved constituents of large dense-cored vesicles and synaptic vesicles intermix in stimulation-induced early endosomes of noradrenergic neurons
P. Partoens, D. Slembrouck, J. Quatacker, P. Baudhuin, P.J. Courtoy, W.P. De Potter; Retrieved constituents of large dense-cored vesicles and synaptic vesicles intermix in stimulation-induced early endosomes of noradrenergic neurons. J Cell Sci 15 March 1998; 111 (6): 681–689. doi: https://doi.org/10.1242/jcs.111.6.681
Download citation file:
Advertisement
Cited by
JCS Journal Meeting 2023: Imaging Cell Dynamics

Our 2023 Journal Meeting on ‘Imaging Cell Dynamics’ will be held from 14-17 May 2023 in Lisbon, Portugal. We have a limited number of spaces left so sign up now! Registration deadline: 31 March.
Call for papers: Cell and Tissue Polarity
-PolarityCFP.png?versionId=4491)
We are welcoming submissions for our next special issue, which will focus on ‘Cell and tissue polarity’ and will be guest edited by David Bryant. Submission deadline: 15 July.
Webinar: Increasing the visibility and impact of your research
-HUBSwebinar.jpg?versionId=4491)
Would you like to increase the visibility and impact of your research and raise your profile internationally? If so, register for the very practical webinar we are running in association with HUBS on 23 February 2023.
Cell scientist to watch: Gautam Dey

We interviewed Gautam Dey, who became a group leader at EMBL in Heidelberg, Germany, in 2021. His lab investigates the fundamental organisational principles and evolutionary dynamics of the nuclear compartment across eukaryotes.
Mechanisms of eukaryotic transcription termination at a glance

Check out our latest Cell Science at a Glance article and accompanying poster for an overview of the current understanding about the mechanisms of transcription termination by the three eukaryotic RNAPs.