As the first step of protein transport along the biosynthetic (secretory/exocytotic) pathway, transport from the endoplasmic reticulum (ER) to the Golgi apparatus has received much attention over the past several decades. The general structural organization underlying this transport process is becoming more defined. The major protein components participating in the budding, pre-docking, and docking/fusion events have been identified and their mechanistic aspects investigated. Conceptually, it is now clear that protein export from the ER is a selective process. Although much remains to be defined or refined, the general picture of this transport step has now emerged.

REFERENCES

Annaert
W. G.
,
Becker
B.
,
Kistner
U.
,
Reth
M.
,
Jahn
R.
(
1997
).
Export of cellubrevin from the endoplasmic reticulum is controlled by BAP31.
J. Cell Biol
139
,
1397
1410
Aridor
M.
,
Bannykh
S. I.
,
Rowe
T.
,
Balch
W. E.
(
1995
).
Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport.
J. Cell Biol
131
,
875
893
Aridor
M.
,
Weissman
J.
,
Bannykh
S.
,
Nuoffer
C.
,
Balch
W. E.
(
1998
).
Cargo selection by the COPII budding machinery during export from the ER.
J. Cell Biol
141
,
61
70
Baker
D.
,
Hicke
L.
,
Rexach
M.
,
Schleyer
M.
,
Schekman
R.
(
1988
).
Reconstitution of SEC gene product-dependent intercompartment protein transport.
Cell
54
,
335
344
Balch
W. E.
,
McCaffery
J. M.
,
Plunter
H.
,
Farquhar
M. G.
(
1994
).
Vesicular stomatitis virus glycoprotein is sorted and concentrated during export from the endoplasmic reticulum.
Cell
77
,
841
852
Bannykh
S. I.
,
Balch
W. E.
(
1997
).
Membrane dynamics at the endoplasmic reticulum-Golgi interface.
J. Cell Biol
138
,
1
4
Bannykh
S. I.
,
Rowe
T.
,
Balch
W. E.
(
1996
).
The organization of endoplasmic reticulum export complexes.
J. Cell Biol
135
,
19
35
Barlowe
C.
,
Orci
L.
,
Yeung
T.
,
Hosobuchi
M.
,
Hamamoto
S.
,
Salama
N.
,
Rexach
M. F.
,
Ravazzola
M.
,
Amherdt
M.
,
Schekman.
R.
(
1994
).
COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum.
Cell
77
,
895
907
Barlowe
C.
(
1997
).
Coupled ER to Golgi transport reconstituted with purified cytosolic proteins.
J. Cell Biol
139
,
1097
1108
Beckers
C. J. M.
,
Keller
D. S.
,
Balch
W. E.
(
1987
).
Semi-intact cells permeable to macromolecules: use in reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex.
Cell
50
,
523
534
Beckers
C. J.
,
Block
M. R.
,
Glick
B. S.
,
Rothman
J. E.
,
Balch
W. E.
(
1989
).
Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein.
Nature
339
,
397
398
Belden
W. J.
,
Barlowe
C.
(
1996
).
Erv25p, a component of COPII-coated vesicles, forms a complex with Emp24p that is required for efficient endoplasmic reticulum to Golgi transport.
J. Biol. Chem
271
,
26939
26946
Bonatti
S.
,
Migliaccio
G.
,
Simons
K.
(
1989
).
Palmitylation of viral membrane glycoprotein takes place after exit from the endoplasmic reticulum.
J. Biol. Chem
264
,
12590
12595
Campbell
J. L.
,
Schekman
R.
(
1997
).
Selective packaging of cargo molecules into endoplasmic reticulum-derived COPII vesicles.
Proc. Nat. Acad. Sci. USA
94
,
837
842
Cao
X.
,
Ballew
N.
,
Barlowe
C.
(
1998
).
Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins.
EMBO J
17
,
2156
2165
Chavrier
P.
,
Parton
R. G.
,
Hauri
H. P.
,
Simons
K.
,
Zerial
M.
(
1990
).
Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments.
Cell
62
,
317
329
Clary
D. O.
,
Griff
I. C.
,
Rothman
J. E.
(
1990
).
SNAPs, a family of NSF attachment proteins involved in intracellular membrane fusion in animals and yeast.
Cell
61
,
709
721
Cosson
P.
,
Letourneur
F.
(
1994
).
Coatomer interaction with di-lysine endoplasmic reticulum retention motifs.
Science
263
,
1629
1631
Dascher
C.
,
Ossig
R.
,
Gallwitz
D.
,
Schmitt
H. D.
(
1991
).
Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily.
Mol. Cell Biol
11
,
872
885
Dascher
C.
,
Matteson
J.
,
Balch
W. E.
(
1994
).
Syntaxin 5 regulates endoplasmic reticulum to Golgi transport.
J. Biol. Chem
269
,
29363
29366
Dominguez
M.
,
Dejgaard
K.
,
Fullekrug
J.
,
Dahan
S.
,
Fazel
A.
,
Paccaud
J. P.
,
Thomas
D. Y.
,
Bergeron
J. J.
,
Nilsson
T.
(
1998
).
gp25L/emp24/p24 protein family members of the cis -Golgi network bind both COP I and II coatomer.
J. Cell Biol
140
,
751
765
Ferro-Novick
S.
,
Jahn
R.
(
1994
).
Vesicle fusion from yeast to man.
Nature
370
,
191
193
Fiedler
K.
,
Simons
K.
(
1994
).
A putative novel class of animal lectins in the secretory pathway homologous to leguminous lectins.
Cell
77
,
625
626
Fiedler
K.
,
Veit
M.
,
Stamnes
M. A.
,
Rothman
J. E.
(
1996
).
Bimodal interaction of coatomer with the p24 family of putative cargo receptors.
Science
273
,
1396
1399
Fiedler
K.
,
Rothman
J. E.
(
1997
).
Sorting determinants in the transmembrane domain of p24 proteins.
J. Biol. Chem
272
,
24739
24742
Graham
T. R.
,
Emr
S. D.
(
1991
).
Compartmental organization of Golgi-specific protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant.
J. Cell Biol
114
,
207
218
Griff
I. C.
,
Schekman
R.
,
Rothman
J. E.
,
Kaiser
C. A.
(
1992
).
The yeast SEC17 gene product is functionally equivalent to mammalian-SNAP protein.
J. Biol. Chem
267
,
12106
12115
Griffiths
G.
,
Simons
K.
(
1986
).
The trans Golgi network: sorting at the exit site of the Golgi complex.
Science
234
,
438
443
Griffiths
G.
,
Ericsson
M.
,
Krijnse-Locker
J.
,
Nilsson
T.
,
Goud
B.
,
Soling
H.-D.
,
Tang
B. L.
,
Wong
S. H.
,
Hong
W.
(
1994
).
Localisation of the KDEL receptor to the Golgi complex and the intermediate compartment in mammalian cells.
J. Cell Biol
127
,
1557
1574
Hardwick
K. G.
,
Pelham
H. R. B.
(
1992
).
SED5 encodes a 39-kD integral membrane protein required for vesicular transport between the ER and the Golgi complex.
J. Cell Biol
119
,
513
521
Hay
J. C.
,
Harald
H.
,
Scheller
R. H.
(
1996
).
Mammalian vesicle trafficking proteins of the endoplasmic reticulum and Golgi apparatus.
J. Biol. Chem
271
,
5671
5679
Hay
J. C.
,
Chao
D. S.
,
Kuo
C. S.
,
Scheller
R. H.
(
1997
).
Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells.
Cell
89
,
147
158
Hong
W.
,
Tang
B. L.
(
1993
).
Protein trafficking along the exocytotic pathway.
BioEssays
15
,
231
238
Hui
N.
,
Nakamura
N.
,
Sonnichsen
B.
,
Shima
D. T.
,
Nilsson
T.
,
Warren
G.
(
1997
).
An isoform of the Golgi t-SNARE, syntaxin 5, with an endoplasmic reticulum retrieval signal.
Mol. Biol. Cell
8
,
1777
1787
Jamieson
J. D.
,
Palade
G. E.
(
1967
).
Intracellular transport of secretory proteins in the pancreatic exocrine cell. I. Role of the peripheral elements of the Golgi complex.
J. Cell Biol
34
,
577
596
Kuehn
M. J.
,
Schekman
R.
,
Ljungdahl
P. O.
(
1996
).
Amino acid permeases require COPII components and the ER resident membrane protein Shr3p for packaging into transport vesicles in vitro.
J. Cell Biol
135
,
585
595
Kuehn
M. J.
,
Herrmann
J. M.
,
Schekman
R.
(
1998
).
COPII-cargo interactions direct protein sorting into ER-derived transport vesicles.
Nature
391
,
187
190
Kuge
O.
,
Dascher
C.
,
Orci
L.
,
Rowe
T.
,
Amherdt
M.
,
Plutner
H.
,
Ravazzola
M.
,
Tanigawa
G.
,
Rothman
J. E.
,
Balch
W. E.
(
1994
).
Sar1 promotes vesicle budding from the endoplasmic reticulum but not Golgi compartments.
J. Cell Biol
125
,
51
65
Lazar
T.
,
Gotte
M.
,
Gallwitz
D.
(
1997
).
Vesicular transport: how many Ypt/Rab-GTPases make a eukaryotic cell?.
Trends Biochem. Sci
22
,
468
472
Lian
J. P.
,
Ferro-Novick
S.
(
1993
).
Bos1p, an integral membrane protein of the endoplasmic reticulum to Golgi transport vesicles, is required for their fusion competence.
Cell
73
,
735
745
Lian
J. P.
,
Stone
S.
,
Jiang
Y.
,
Lyons
P.
,
Ferro-Novick.
S.
(
1994
).
Ypt1p implicated in v-SNARE activation.
Nature
372
,
698
701
Lodish
H. F.
(
1988
).
Transport of secretory and membrane glycoproteins from the rough endoplasmic reticulum to the Golgi. A rate-limiting step in protein maturation and secretion.
J. Biol. Chem
263
,
2107
2110
Lotti
L. V.
,
Torrisi
M. R.
,
Pascale
M. C.
,
Bonatti
S.
(
1992
).
Immunocytochemical analysis of the transfer of vesicular stomatitis virus G glycoprotein from the intermediate compartment to the Golgi complex.
J. Cell Biol
118
,
43
50
Lupashin
V. V.
,
Hamamoto
S.
,
Schekman
R. W.
(
1996
).
Biochemical requirements for the targeting and fusion of ER-derived transport vesicles with purified yeast Golgi membranes.
J. Cell Biol
132
,
277
289
Lupashin
V. V.
,
Waters
M. G.
(
1997
).
t-SNARE activation through transient interaction with a rab-like guanosine triphosphatase.
Science
276
,
1255
1258
Matsuoka
K.
,
Orci
L.
,
Amherdt
M.
,
Bednarek
S. Y.
,
Hamamoto
S.
,
Schekman
R.
,
Yeung
T.
(
1998
).
COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes.
Cell
93
,
263
275
Mayer
A.
,
Wickner
W.
,
Haas
A.
(
1996
).
Sec18p (NSF)-driven releaseof Sec17p (alpha-SNAP) can precede docking and fusion of yeast vacuoles.
Cell
85
,
83
94
Mayer
A.
,
Wickner
W.
(
1997
).
Docking of yeast vacuoles is catalyzed by the Ras-like GTPase Ypt7p after symmetric priming by Sec18p (NSF).
J. Cell Biol
136
,
307
317
McNew
J. A.
,
Sogaard
M.
,
Lampen
N. M.
,
Machida
S.
,
Ye
R. R.
,
Lacomis
L.
,
Tempst
P.
,
Rothman
J. E.
,
Sollner
T. H.
(
1997
).
Ykt6p, a prenylated SNARE essential for endoplasmic reticulum-Golgi transport.
J. Biol. Chem
272
,
17776
17783
Mellman
I.
,
Simons
K.
(
1992
).
The Golgi complex: in vitro veritas?.
Cell
68
,
829
840
Mizuno
M.
,
Singer
S. J.
(
1993
).
A soluble secretory protein is first concentrated in the endoplasmic reticulum before transfer to the Golgi apparatus.
Proc. Nat. Acad. Sci. USA
90
,
5732
5736
Nagahama
M.
,
Orci
L.
,
Ravazzola
M.
,
Amherdt
M.
,
Lacomis
L.
,
Tempst
P.
,
Rothman
J. E.
,
Söllner
T. H.
(
1996
).
A v-SNARE implicated in intra-Golgi transport.
J. Cell Biol
133
,
507
516
Newman
A. P.
,
Shim
J.
,
Ferro-Novick
S.
(
1990
).
BET1, BOS1 and SEC22 are members of a group of interacting yeast genes required for transport from the endoplasmic reticulum to the Golgi complex.
Mol. Cell Biol
10
,
3405
3414
Nichols
W. C.
,
Seligsohn
U.
,
Zivelin
A.
,
Terry
V. H.
,
Hertel
C. E.
,
Wheatley
M. A.
,
Moussalli
M. J.
,
Hauri
H. P.
,
Ciavarella
N.
,
Kaufman
R. J.
,
Ginsburg
D.
(
1998
).
Mutations in the ER-Golgi intermediate compartment protein ERGIC-53 cause combined deficiency of coagulation factors V and VIII.
Cell
93
,
61
70
Nishimura
N.
,
Balch
W. E.
(
1997
).
A di-acidic signal required for selective export from the endoplasmic reticulum.
Science
277
,
556
558
Novick
P.
,
Schekman
R.
(
1979
).
Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae.
Proc. Nat. Acad. Sci. USA
76
,
1858
1862
Novick
P.
,
Field
C.
,
Schekman
R.
(
1980
).
Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway.
Cell
21
,
205
215
Oprins
A.
,
Duden
R.
,
Kreis
T. E.
,
Geuze
H. J.
,
Slot
J. W.
(
1993
).
-COP localizes mainly to the cis -Golgi side in exocrine pancreas.
J. Cell Biol
121
,
49
59
Orci
L.
,
Ravazzola
M.
,
Meda
P.
,
Holcomb
C.
,
Moore
H. P.
,
Hicke
L.
,
Schekman
R.
(
1991
).
Mammalian Sec23p homologue is restricted to the endoplasmic reticulum transitional cytoplasm.
Proc. Nat. Acad. Sci. USA
88
,
8611
8615
Ossig
R.
,
Dascher
C.
,
Trepte
H.-H.
,
Schmitt
H. D.
,
Gallwitz
D.
(
1991
).
The yeast SLY genes products, suppressors of defects in the essential GTP-binding Ypt1 protein, may act in endoplasmic reticulum-to-Golgi transport.
Mol. Cell Biol
11
,
2980
2993
Paccaud
J. P.
,
Reith
W.
,
Carpentier
J. L.
,
Ravazzola
M.
,
Amherdt
M.
,
Schekman
R.
,
Orci
L.
(
1996
).
Cloning and functional characterization of mammalian homologues of the COPII component Sec23.
Mol. Biol. Cell
7
,
1535
1546
Paek
I.
,
Orci
L.
,
Ravazzola
M.
,
Erdjument-Bromage
H.
,
Amherdt
M.
,
Tempst
P.
,
Sollner
T. H.
,
Rothman
J. E.
(
1997
).
ERS-24, a mammalian v-SNARE implicated in vesicle traffic between the ER and the Golgi.
J. Cell Biol
137
,
1017
1028
Palade
G. E.
(
1975
).
Intracellular aspects of the processing of protein synthesis.
Science
189
,
347
354
Peter
F.
,
Wong
S. H.
,
Subramaniam
V. N.
,
Tang
B. L.
,
Hong
W.
(
1998
).
-SNAP but not -SNAP is necessary for ER-Golgi transport after vesicle budding and the Rab1-requiring step but before the EGTA-sensitive step.
J. Cell Sci
111
,
2625
2633
Pfeffer
S. R.
,
Rothman
J. E.
(
1987
).
Biosynthetic protein transport and sorting by the endoplasmic reticulum and Golgi.
Annu. Rev. Biochem
56
,
829
852
Pind
S. N.
,
Nuoffer
C.
,
McCaffery
J. M.
,
Plutner
H.
,
Davidson
H. W.
,
Farquhar
M. G.
,
Balch
W. E.
(
1994
).
Rab1 and Ca2+ are required for the fusion of carrier vesicles mediating endoplasmic reticulum to Golgi transport.
J. Cell Biol
125
,
239
252
Plutner
H.
,
Cox
A. D.
,
Pind
S.
,
Khosravi-Far
R.
,
Bourne
J. R.
,
Schwaninger
R.
,
Der
C. J.
,
Balch
W. E.
(
1991
).
Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.
J. Cell Biol
115
,
31
43
Plutner
H.
,
Davidson
H. W.
,
Saraste
J.
,
Balch
W. E.
(
1992
).
Morphological analysis of protein transport from the ER to the Golgimembrane in digitonin-permeabilized cells: role of the p58 containing compatment.
J. Cell Biol
119
,
1097
1116
Presley
J. F.
,
Cole
N. B.
,
Schroer
T. A.
,
Hirschberg
K.
,
Zaal
K. J.
,
Lippincott-Schwartz
J.
(
1997
).
ER-to-Golgi transport visualized in living cells.
Nature
389
,
81
85
Pryer
N. K.
,
Wuestehube
L. J.
,
Schekman
R.
(
1992
).
Vesicle-mediated protein sorting.
Annu. Rev. Biochem
61
,
471
516
Rexach
M. F.
,
Schekman
R. W.
(
1991
).
Distinct biochemical requirements for budding, targeting and fusion of ER-derived transport vesicles.
J. Cell Biol
114
,
219
229
Rexach
M. F.
,
Latterich
M.
,
Schekman
R. W.
(
1994
).
Characterization of endoplasmic reticulum-derived transport vesicles.
J. Cell Biol
126
,
1133
1148
Rojo
M.
,
Pepperkok
R.
,
Emery
G.
,
Kellner
R.
,
Stang
E.
,
Parton
R. G.
,
Gruenberg
J.
(
1997
).
Involvement of the transmembrane protein p23 in biosynthetic protein transport.
J. Cell Biol
139
,
1119
1135
Rowe
T.
,
Aridor
M.
,
McCaffery
J. M.
,
Plutner
H.
,
Nuoffer
C.
,
Balch
W. E.
(
1996
).
COPII vesicles derived from mammalian endoplasmic reticulum microsomes recruit COPI.
J. Cell Biol
135
,
895
911
Rowe
T.
,
Dascher
C.
,
Bannykh
S.
,
Plutner
H.
,
Balch
W. E.
(
1998
).
Role of vesicle-associated syntaxin 5 in the assembly of pre-Golgi intermediates.
Science
279
,
696
700
Rothman
J. E.
,
Warren
G.
(
1994
).
Implications of the SNARE hypothesis for intracellular membrane topology and dynamics.
Curr. Biol
4
,
220
233
Rothman
J. E.
,
Wieland
F. T.
(
1996
).
Protein sorting by transport vesicles.
Science
272
,
227
234
Ruohola
H.
,
Kabcenell
A. K.
,
Ferro-Novick
S.
(
1988
).
Reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex in yeast: the acceptor Golgi compartment is defective in the sec23 mutant.
J. Cell Biol
107
,
1465
1476
Sacher
M.
,
Jiang
Y.
,
Barrowman
J.
,
Scarpa
A.
,
Burston
J.
,
Zhang
L.
,
Schieltz
D.
,
Yates
III JR
,
Abeliovich
H.
,
Ferro-Novick
S.
(
1998
).
TRAPP, a highly conserved novel complex on the cis -Golgi that mediates vesicle docking and fusion.
EMBO J
17
,
2494
2503
Sapperstein
S. K.
,
Lupashin
V. V.
,
Schmitt
H. D.
,
Waters
M. G.
(
1996
).
Assembly of the ER to Golgi SNARE complex requires Uso1p.
J. Cell Biol
132
,
755
767
Saraste
J.
,
Kuismanen
E.
(
1984
).
Pre-and post-Golgi vacuoles operates in the transport of Semliki Forest virus membrane glycoproteins to the cell surface.
Cell
38
,
535
549
Saraste
J.
,
Palade
G. E.
,
Farquhar
M. G.
(
1987
).
Antibodies to rat pancreas Golgi subfractions: Identification of a 58 kD cis -Golgi protein.
J. Cell Biol
105
,
2021
2029
Saraste
J.
,
Svensson
K.
(
1991
).
Distribution of the intermediate elements operating in ER to Golgi transport.
J. Cell Sci
100
,
415
430
Scales
S. J.
,
Pepperkok
R.
,
Kreis
T. E.
(
1997
).
Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COPII and COPI.
Cell
90
,
1137
1148
Schekman
R.
,
Orci
L.
(
1996
).
Coat proteins and vesicle budding.
Science
271
,
1526
1532
Schekman
R.
,
Mellman
I.
(
1997
).
Does COPI go both ways.
Cell
90
,
197
200
Scheller
R. H.
(
1995
).
Membrane trafficking in the presynaptic nerve terminal.
Neuron
14
,
893
897
Schimmoller
F.
,
Singer-Kruger
B.
,
Schroder
S.
,
Kruger
U.
,
Barlowe
C.
,
Riezman
H.
(
1995
).
The absence of Emp24p, a component of ER-derived COPII-coated vesicles, causes a defect in transport of selected proteins to the Golgi.
EMBO J
14
,
1329
1339
Schweizer
A.
,
Fransen
J. A. M.
,
Bachi
T.
,
Ginsel
L.
,
Hauri
H.-P.
(
1988
).
Identification, by a monoclonal antibody, of a 53 kD protein associated with a tubulo-vesicular compartment at the cis -side of the Golgi apparatus.
J. Cell Biol
107
,
1643
1653
Schweizer
A.
,
Fransen
J. A. M.
,
Matter
K.
,
Kreis
T. E.
,
Ginsel.
L.
,
Hauri
H.-P.
(
1990
).
Identification of an intermediate compartment involved in protein transport from endoplasmic reticulum to Golgi apparatus.
Eur. J. Cell Biol
53
,
185
196
Shaywitz
D. A.
,
Orci
L.
,
Ravazzola
M.
,
Swaroop
A.
,
Kaiser
C. A.
(
1995
).
Human SEC13Rp functions in yeast and is located on transport vesicles budding from the endoplasmic reticulum.
J. Cell Biol
128
,
769
777
Shaywitz
D. A.
,
Espenshade
P. J.
,
Gimeno
R. E.
,
Kaiser
C. A.
(
1997
).
COPII subunit interactions in the assembly of the vesicle coat.
J. Biol. Chem
272
,
25413
25416
Simons
K.
,
Virta
H.
(
1987
).
Perforated MDCK cells support intracellular transport.
EMBO J
8
,
2241
2247
Slot
J. W.
,
Geuze
J. J.
(
1976
).
Synthesis and intracellular transport of proteins in the exocrine pancreas of the frog (Rana esculenta). II. An in vitro study of the transport process and the influence of temperature.
Cell Tissue Res
167
,
147
165
Slot
J. W.
,
Geuze
H. J.
(
1983
).
Immunoelectron microscopic exploration of the Golgi complex.
J. Histochem. Cytochem
31
,
1049
1056
Sögaard
M.
,
Tani
K.
,
Ye
R. B.
,
Geromanos
S.
,
Tempst
P.
,
Kirchhausen
T.
,
Rothman
J. E.
,
Söllner
T.
(
1994
).
A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles.
Cell
78
,
937
948
Sohn
K.
,
Orci
L.
,
Ravazzola
M.
,
Amherdt
M.
,
Bremser
M.
,
Lottspeich
F.
,
Fiedler
K.
,
Helms
J. B.
,
Wieland
F. T.
(
1996
).
A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding.
J. Cell Biol
135
,
1239
1248
Söllner
T.
,
Whiteheart
S. W.
,
Brunner
M.
,
Erdjument-Bromage
H.
,
Geromanos
S.
,
Tempst
P.
,
Rothman
J. E.
(
1993
).
SNAP receptors implicated in vesicle targeting and fusion.
Nature
362
,
318
324
Stamnes
M. A.
,
Craighead
M. W.
,
Hoe
M. H.
,
Lampen
N.
,
Geromanos
S.
,
Tempst
P.
,
Rothman
J. E.
(
1995
).
An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding.
Proc. Nat. Acad. Sci. USA
92
,
8011
8015
Stinchcombe
J. C.
,
Nomoto
H.
,
Cutler
D. F.
,
Hopkins.
C. R.
(
1995
).
Anterograde and retrograde traffic between the rough endoplasmic reticulum and the Golgi complex.
J. Cell Biol
131
,
1387
1401
Subramaniam
V. N.
,
Krijnse-Locker
J.
,
Tang
B. L.
,
Ericsson
M.
,
Yosoff
A. R. bin M.
,
Griffiths
G.
,
Hong
W.
(
1995
).
Monoclonal antibody HFD9 identifies a novel 28 kDa integral membrane protein on the cis -Golgi.
J. Cell Sci
108
,
2405
2414
Subramaniam
V. N.
,
Peter
F.
,
Philip
R.
,
Wong
S. H.
,
Hong
W.
(
1996
).
GS28, a 28-kilodalton Golgi SNARE that participates in ER-Golgi transport.
Science
272
,
1161
1163
Sudhof
T. C.
(
1995
).
The synaptic vesicle cycle: a cacade of protein-protein interactions.
Nature
375
,
645
653
Tang
B. L.
,
Wong
S. H.
,
Qi
X. L.
,
Low
S. H.
,
Hong
W.
(
1993
).
Molecular cloning, characterization, subcellular localization and dynamicsof p23, the mammalian KDEL receptor.
J. Cell Biol
120
,
325
338
Tang
B. L.
,
Low
S. H.
,
Hauri
H.-P.
,
Hong
W.
(
1995
).
Segregation of ERGIC53 and the mammalian KDEL receptor upon exit from the 15degC compartment.
Eur. J. Cell Biol
68
,
398
410
Tang
B. L.
,
Peter
F.
,
Krijnse-Locker
J.
,
Low
S. H.
,
Griffiths
G.
,
Hong
W.
(
1997
).
The mammalian homolog of yeast Sec13p is enriched in the intermediate compartment and is essential for protein transport from the endoplasmic reticulum to the Golgi apparatus.
Mol. Cell. Biol
17
,
256
266
Tisdale
E. J.
,
Balch
W. E.
(
1996
).
Rab2 is essential for the maturation of pre-Golgi intermediates.
J. Biol. Chem
271
,
29372
29379
Tisdale
E. J.
,
Plutner
H.
,
Matteson
J.
,
Balch
W. E.
(
1997
).
p53/58 binds COPI and is required for selective transport through the early secretory pathway.
J. Cell Biol
137
,
581
593
Ungermann
C.
,
Nichols
B. J.
,
Pelham
H. R.
,
Wickner
W.
(
1998
).
A vacuolar v-t-SNARE complex, the predominant form in vivo and on isolated vacuoles, is disassembled and activated for docking and fusion.
J. Cell Biol
140
,
61
69
VanRheenen
S. M.
,
Cao
X.
,
Lupashin
V. V.
,
Barlowe
C.
,
Waters
M. G.
(
1998
).
Sec35p, a novel peripheral membrane protein, is required for ER to Golgi vesicle docking.
J. Cell Biol
141
,
1107
1119
Waters
M. G.
,
Clary
D. O.
,
Rothman
J. E.
(
1992
).
A novel 115-kD peripheral membrane protein is required for intercisternal transport in the Golgi stack.
J. Cell Biol
118
,
1015
1026
Weber
T.
,
Zemelman
B. V.
,
McNew
J. A.
,
Westermann
B.
,
Gmachl
M.
,
Parlati
F.
,
Söllner
T. H.
,
Rothman
J. E.
(
1998
).
SNAREpins: minimal machinery for membrane fusion.
Cell
92
,
759
772
Wieland
F. T.
,
Gleason
M. L.
,
Serafini
T. A.
,
Rothman
J. E.
(
1987
).
The rate of bulk flow from the endoplasmic reticulum to the cell surface.
Cell
50
,
289
300
Zhang
T.
,
Wong
S. H.
,
Tang
B. L.
,
Xu
Y.
,
Peter
F.
,
Hong
W.
(
1997
).
The mammalian protein (rbet1) homologous to yeast Bet1p is primarily associated with the pre-Golgi intermediate compartment and is involved in vesicular transport from the endoplasmic reticulum to the Golgi apparatus.
J. Cell Biol
139
,
1157
1168
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