A 32P-labeling strategy was developed to study the interaction(s) in tissue culture cells between proteins and nucleic acids. Interphase and mitotic nuclear lamins were studied in Drosophila Kc cells. After bromodeoxyuridine incorporation and in vivo photo-crosslinking with 366 nm light, it was found that interphase lamins were associated with nucleic acid. Interactions with DNA as well as RNA were detected. In contrast, interaction of nucleic acids with mitotic lamin was not observed. Photo-crosslinking in the presence of antibiotics distamycin and/or chromomycin suggested that interphase lamins interacted with both A-T-rich DNA and G-C-rich DNA; interactions with G-C-rich DNA predominated. These results have implications for understanding the interphase organization of the higher eukaryotic cell nucleus as well as the transition of cells from interphase to mitosis. A model of nuclear organization, consistent with our results, is proposed.
In vivo association of lamins with nucleic acids in Drosophila melanogaster
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R. Rzepecki, S.S. Bogachev, E. Kokoza, N. Stuurman, P.A. Fisher; In vivo association of lamins with nucleic acids in Drosophila melanogaster. J Cell Sci 1 January 1998; 111 (1): 121–129. doi: https://doi.org/10.1242/jcs.111.1.121
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