Desmocollins are cadherin-like glycoproteins involved in cell adhesion and plaque formation in desmosome junctions. Three distinct isoforms, the products of different genes, have been found in bovine tissues. We have reported previously that one of these, DSC3, is expressed only in basal and lower suprabasal layers of stratified epithelia. Using RT-PCR we have now obtained the complete cDNA coding sequence of mature bovine DSC3. It has alternatively spliced ‘a’ and ‘b’ forms found in other desmocollins but is unique in having a 43 instead of a 46 base pair exon. We have characterised a monoclonal antibody, 07–4G, which is specific for the Dsc3 protein, recognising an epitope in the extracellular domain. Immunofluorescent staining with 07–4G confirms that this isoform is found only in stratified epithelia, being strongly expressed in the basal cell layers of these tissues. The intensity of expression fades gradually in the suprabasal layers and disappears completely below the upper limit of desmosome expression. These results suggest that Dsc3 plays an important role in cell epithelial differentiation.

Amagai
M.
,
Karpati
S.
,
Klaus-Kovtun
V.
,
Udey
M. C.
,
Stanley
J. R.
(
1994
).
Extracellular domain of pemphigus vulgaris antigen (desmoglein 3) mediates weak homophilic adhesion.
J. Invest. Dermatol
102
,
402
408
Arnemann
J.
,
Sullivan
K. H.
,
Magee
A. I.
,
King
I. A.
,
Buxton
R. S.
(
1993
).
Stratification related expression of isoforms of the desmosomal cadherins in human epidermis.
J. Cell Sci
104
,
741
750
Blaschuk
O. W.
,
Sullivan
R.
,
David
S.
,
Pouliot
Y.
(
1990
).
Identification of a cadherin cell adhesion recognition sequence.
Dev. Biol
139
,
227
229
Buxton
R. S.
,
Magee
A. I.
(
1992
).
Structure and interactions of desmosomal and other cadherins.
Semin. Cell Biol
3
,
157
167
Buxton
R. S.
,
Cowin
P.
,
Franke
W. W.
,
Garrod
D. R.
,
Green
K. J.
,
King
I. A.
,
Koch
P. J.
,
Magee
A. I.
,
Rees
D. A.
,
Stanly
J. R.
,
Steinberg
M. S.
(
1993
).
Nomenclature of the desmosomal cadherins.
J. Cell Biol
121
,
481
483
Chomczynski
P.
,
Sacchi
N.
(
1987
).
Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
Anal. Biochem
162
,
156
159
Collins
J. E.
,
Legan
P. K.
,
Kenny
T. P.
,
MacGarvie
J.
,
Holton
J. L.
,
Garrod
D. R.
(
1991
).
Cloning and sequence analysis of desmosomal glycoproteins 2 and 3 (desmocollins): cadherin-like desmosomal adhesion molecules with heterogenous cytoplasmic domains.
J. Cell Biol
113
,
381
391
Cowin
P.
,
Mattey
D. L.
,
Garrod
D. R.
(
1984
).
Identification of desmosomal surface components (desmocollins) and inhibition of desmosome formation by specific FAB.
J. Cell Sci
70
,
41
60
Garrod
D. R.
(
1993
).
Desmosomes and hemidesmosomes.
Curr. Opin. Cell Biol
5
,
30
40
Gorbsky
G.
,
Steinberg
M. S.
(
1981
).
Isolation of the intercellular glycoproteins of desmosomes.
J. Cell Biol
90
,
243
248
Holton
J. L.
,
Kenny
T. P.
,
Legan
P. K.
,
Collins
J. E.
,
Keen
J. N.
,
Sharma
R.
,
Garrod
D. R.
(
1990
).
Desmosomal glycoproteins 2 and 3 (desmocollins) show N-terminal similarity to calcium-dependent cell-cell adhesion molecules.
J. Cell Sci
97
,
239
246
Kawamura
K.
,
Watanabe
K.
,
Suzuki
T.
,
Yamakawa
T.
,
Kamiyama
T.
,
Nakagawa
H.
,
Tsurufuji
S.
(
1994
).
cDNA cloning and expression of a novel human desmocollin.
J. Biol. Chem
269
,
26295
26302
King
I. A.
,
Tabiowo
A.
,
Purkis
P.
,
Leigh
I.
,
Magee
A. I.
(
1993
).
Expression of distinct desmocollin isoforms in human epidermis.
J. Invest. Dermatol
100
,
373
379
Koch
P. J.
,
Goldschmidt
M. D.
,
Walsh
M. J.
,
Zimbelmann
R.
,
Schmelz
M.
,
Franke
W. W.
(
1991
).
Amino acid sequence of bovine muzzle epithelial desmocollin derived from cloned cDNA: A novel subtype of desmosomal cadherins.
Differentiation
47
,
29
36
Koch
P. J.
,
Goldschmidt
M. D.
,
Zimblemann
R.
,
Troyanovsky
R.
,
Franke
W. W.
(
1992
).
Complexity and expression patterns of the desmosomal cadherins.
Proc. Nat. Acad. Sci. USA
89
,
353
357
Koch
P. J.
,
Franke
W. W.
(
1994
).
Desmosomal cadherins: another growing multigene family of adhesion molecules.
Curr. Opin. Cell Biol
6
,
682
687
Kopan
R.
,
Fuchs
E.
(
1989
).
The use of retinoic acid to probe the relation between hyperproliferation-associated keratins and cell proliferation in normal and malignant cells.
J. Cell Biol
109
,
295
307
Laemmli
U. K.
(
1970
).
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature
227
,
680
685
Legan
P. K.
,
Collins
J. E.
,
Garrod
D. R.
(
1992
).
The molecular biology of desmosomes and hemidesmosomes: ‘What's in a name?’.
BioEssays
,
14
,
385
393
Legan
P. K.
,
Yue
K. K. M.
,
Chidgey
M. A. J.
,
Holton
J. L.
,
Wilkinson
R. W.
,
Garrod
D. R.
(
1994
).
The bovine desmocollin family: a new gene and expression patterns reflecting epithelial cell proliferation and differentiation.
J. Cell Biol
126
,
507
518
Lorimer
J. E.
,
Hall
L. S.
,
Clarke
J. P.
,
Collins
J. E.
,
Buxton
R. S.
,
Fleming
T. P.
,
Garrod
D. R.
(
1994
).
Cloning, sequence analysis and expression pattern of mouse desmocollins (DSC2): a cadherin-like adhesion molecule.
Mol. Membr. Biol
11
,
229
236
Magee
A. I.
,
Buxton
R. S.
(
1992
).
Transmembrane molecular assemblies regulated by the greater cadherin family.
Curr. Opin. Cell Biol
3
,
854
861
Mattey
D. L.
,
Burdge
G.
,
Garrod
D. R.
(
1990
).
Development of desmosomal adhesion between MDCK cells following calcium switching.
J. Cell Sci
97
,
689
704
Mechanic
S.
,
Raynor
K.
,
Hill
J. E.
,
Cowin
P.
(
1991
).
Desmocollins form a distinct subset of the cadherin family of cell adhesion molecules.
Proc. Nat. Acad. Sci. USA
88
,
4476
4480
Nose
A.
,
Tsuji
K.
,
Takeichi
M.
(
1990
).
Localization of specificity determining sites in cadherin cell adhesion molecules.
Cell
61
,
147
155
Parker
A. E.
,
Wheeler
G. N.
,
Arnemann
J.
,
Pidsley
S. C.
,
Rutman
A. J.
,
Thomas
C. L.
,
Ataliotis
P.
,
Rees
D.
,
Magee
A. I.
,
Buxton
R. S.
(
1991
).
Desmosomal glycoproteins II and III: Cadherin-like junctional molecules generated by alternative splicing.
J. Biol. Chem
266
,
10438
10445
Parrish
E. P.
,
Garrod
D. R.
,
Mattey
D. L.
,
Hand
L.
,
Steart
P. V.
,
Weller
R. O.
(
1987
).
Mouse antisera specific for desmosomal adhesion molecules of suprabasal skin cells, meninges and meningioma.
Proc. Nat. Acad. Sci. USA
83
,
265
273
Parrish
E. P.
,
Marston
J. E.
,
Mattey
D. L.
,
Measures
H. M.
,
Venning
R.
,
Garrod
D. R.
(
1990
).
Size heterogeneity, phosphorylation and transmembrane organisation of desmosomal glycoproteins 2 and 3 (desmocollins) in MDCK cells.
J. Cell Sci
96
,
239
248
Sanger
F.
,
Nicklen
S.
,
Coulson
A. R.
(
1977
).
DNA sequencing with chain terminating inhibitors.
Proc. Nat. Acad. Sci. USA
74
,
5463
5467
Theis
D. G.
,
Koch
P. J.
,
Franke
W. W.
(
1993
).
Differential synthesis of type 1 and type 2 desmocollin mRNAs in human stratified epithelia.
Int. J. Dev. Biol
37
,
101
110
Tindall
K. R.
,
Kunjek
T. A.
(
1988
).
Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase.
Biochemistry
27
,
6008
6013
Troyanovsky
S. M.
,
Eshkind
L. G.
,
Troyanovsky
R. G.
,
Leube
R. E.
,
Franke
W. W.
(
1993
).
Contributions of cytoplasmic domains of desmosomal cadherins to desmosome assembly and intermediate filament anchorage.
Cell
72
,
561
574
Wheeler
G. N.
,
Buxton
R. S.
,
Parker
A. E.
,
Arnemann
J.
,
Rees
D. A.
,
King
I. A.
,
Magee
A. I.
(
1991
).
Desmosomal glycoproteins I, II and III: novel members of the cadherin superfamily.
Biochem Soc. Trans
19
,
1060
1064
This content is only available via PDF.